Dystrophin (DMD) (NM_004021) Human Over-expression Lysate
CAT#: LY418281
Transient overexpression lysate of dystrophin (DMD), transcript variant Dp140b
Size: 20 ug
View other "Dystrophin" proteins (16)
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Specifications
Product Data | |
Species | Human |
Applications | WB |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC213926 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Accession Number | NM_004021, NP_004012 |
Other Names | BMD; CMD3B; DXS142; DXS164; DXS206; DXS230; DXS239; DXS268; DXS269; DXS270; DXS272; MRX85 |
Predicted MW | 143.6 kDa |
Components | 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT) |
Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
Reference Data | |
RefSeq | NP_004012 |
Locus ID | 1756 |
Cytogenetics | Xp21.2-p21.1 |
Protein Pathways | Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM), Viral myocarditis |
Documents
Product Manuals |
FAQs |
SDS |
Resources
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC418275 | DMD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 206.00 |
|
LC418276 | DMD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 206.00 |
|
LC418277 | DMD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 206.00 |
|
LC418278 | DMD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 206.00 |
|
LC418279 | DMD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 134.00 |
|
LC418281 | DMD HEK293T cell transient overexpression lysate (as WB positive control) |
USD 206.00 |
|
LY418275 | Transient overexpression lysate of dystrophin (DMD), transcript variant Dp71 |
USD 665.00 |
|
LY418276 | Transient overexpression lysate of dystrophin (DMD), transcript variant Dp71b |
USD 665.00 |
|
LY418277 | Transient overexpression lysate of dystrophin (DMD), transcript variant Dp71a |
USD 665.00 |
|
LY418278 | Transient overexpression lysate of dystrophin (DMD), transcript variant Dp71ab |
USD 665.00 |
|
LY418279 | Transient overexpression lysate of dystrophin (DMD), transcript variant Dp40 |
USD 436.00 |
|
PH313926 | DMD MS Standard C13 and N15-labeled recombinant protein (NP_004012) |
USD 3,255.00 |
|
PH315529 | DMD MS Standard C13 and N15-labeled recombinant protein (NP_004009) |
USD 3,255.00 |
|
TP313926 | Recombinant protein of human dystrophin (DMD), transcript variant Dp140b, 20 µg |
USD 867.00 |
|
TP315529 | Recombinant protein of human dystrophin (DMD), transcript variant Dp71ab, 20 µg |
USD 867.00 |
|
TP762385 | Purified recombinant protein of Human dystrophin (DMD), transcript variant Dp427m, Lys3200-Thr3684, with N-terminal His tag, expressed in E.coli, 50ug |
USD 249.00 |
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