p90 Autoantigen (KIAA1524) (NM_020890) Human Over-expression Lysate
Specifications
Product Data | |
Species | Human |
Applications | WB |
Expression Host | HEK293T |
Expression cDNA Clone or AA Sequence | TrueORF Clone RC219918 |
Tag | C-Myc/DDK |
Detection Antibodies | Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody (TA50011-100) |
Accession Number | NM_020890, NP_065941 |
Other Names | KIAA1524; p90 |
Predicted MW | 102 kDa |
Components | 1 vial of 100 µg gene specific transient over-expression cell lysate in RIPA buffer 1 vial of 100 µg whole HEK293T cell lysate in RIPA buffer 1 vial of 250ul 2xSDS Sample Buffer (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT) |
Storage | The lysate is shipped with dry ice. Upon receiving, store the sample at -80°C. Also after dilution, the protein sample should be aliquoted and stored at -80°C for long term storage. Avoid repeated freeze-thaw cycles. Lysate samples can be diluted with 2xSDS Sample Buffer provided. Lysate samples are stable for 12 months from the date of receipt when stored at -80°C. |
Preparation | HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent (TT200002) and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). |
Reference Data | |
RefSeq | NP_065941 |
Locus ID | 57650 |
Cytogenetics | 3q13.13 |
Protein Families | WB |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Recombinant Protein Resources |
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
LC412250 | KIAA1524 HEK293T cell transient overexpression lysate (as WB positive control) |
USD 206.00 |
|
PH319918 | KIAA1524 MS Standard C13 and N15-labeled recombinant protein (NP_065941) |
USD 3,255.00 |
|
TP319918 | Purified recombinant protein of Homo sapiens KIAA1524 (KIAA1524), 20 µg |
USD 867.00 |
{0} Product Review(s)
Be the first one to submit a review