SPC24 (NM_182513) Human Mass Spec Standard

CAT#: PH311241

SPC24 MS Standard C13 and N15-labeled recombinant protein (NP_872319)

Product Images

Coomassie blue staining of purified SPC24 protein (Cat# TP311241). The protein was produced from HEK293T cells transfected with SPC24 cDNA clone (Cat# RC211241) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC211241
• Predicted MW: 22.4 kDa
• Protein Sequence:
  Sequence Link

  >RC211241 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAFRDIEEVSQGLLSLLGANRAEAQQRRLLGRHEQVVERLLETQDGAEKQLREILTMEKEVAQSLLNAK
  EQVHQGGVELQQLEAGLQEAGEEDTRLKASLLQLTRELEELKEIEADLERQEKEVDEDTTVTIPSAVYVA
  QLYHQVSKIEWDYECEPGMVKGIHHGPSVAQPIHLDSTQLSRKFISDYLWSLVDTEW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_872319
• RefSeq Size: 654
• RefSeq ORF: 591
• Synonyms: SPBC24
• Locus ID: 147841
• UniProt ID: Q8NBT2
• Cytogenetics: 19p13.2
• Summary: Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity (PubMed:14738735). Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore (PubMed:14738735). The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules (PubMed:23085020).[UniProtKB/Swiss-Prot Function]
• Protein Families: Druggable Genome