DCTN3 (NM_007234) Human Mass Spec Standard

CAT#: PH310658

DCTN3 MS Standard C13 and N15-labeled recombinant protein (NP_009165)

Product Images

Coomassie blue staining of purified DCTN3 protein (Cat# TP310658). The protein was produced from HEK293T cells transfected with DCTN3 cDNA clone (Cat# RC210658) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC210658
• Predicted MW: 21.1 kDa
• Protein Sequence:
  Sequence Link

  >RC210658 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAGLTDLQRLQARVEELERWVYGPGGARGSRKVADGLVKVQVALGNISSKRERVKILYKKIEDLIKYLDP
  EYIDRIAIPDASKLQFILAEEQFILSQVALLEQVNALVPMLDSAHIKAVPEHAARLQRLAQIHIQQQDQC
  VEITEESKALLEEYNKTTMLLSKQFVQWDELLCQLEAATQVKPAEE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_009165
• RefSeq Size: 857
• RefSeq ORF: 558
• Synonyms: DCTN-22; DCTN22
• Locus ID: 11258
• UniProt ID: O75935
• Cytogenetics: 9p13.3
• Summary: This gene encodes the smallest subunit of dynactin, a macromolecular complex consisting of 10 subunits ranging in size from 22 to 150 kD. Dynactin binds to both microtubules and cytoplasmic dynein. It is involved in a diverse array of cellular functions, including ER-to-Golgi transport, the centripetal movement of lysosomes and endosomes, spindle formation, cytokinesis, chromosome movement, nuclear positioning, and axonogenesis. This subunit, like most other dynactin subunits, exists only as a part of the dynactin complex. It is primarily an alpha-helical protein with very little coiled coil, and binds directly to the largest subunit (p150) of dynactin. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]