GDA (NM_004293) Human Mass Spec Standard

CAT#: PH308771

GDA MS Standard C13 and N15-labeled recombinant protein (NP_004284)

Product Images

Coomassie blue staining of purified GDA protein (Cat# TP308771). The protein was produced from HEK293T cells transfected with GDA cDNA clone (Cat# RC208771) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC208771
• Predicted MW: 50.8 kDa
• Protein Sequence:
  Sequence Link

  >RC208771 representing NM_004293
  Red=Cloning site Green=Tags(s)
  
  MCAAQMPPLAHIFRGTFVHSTWTCPMEVLRDHLLGVSDSGKIVFLEEASQQEKLAKEWCFKPCEIRELSH
  HEFFMPGLVDTHIHASQYSFAGSSIDLPLLEWLTKYTFPAEHRFQNIDFAEEVYTRVVRRTLKNGTTTAC
  YFATIHTDSSLLLADITDKFGQRAFVGKVCMDLNDTFPEYKETTEESIKETERFVSEMLQKNYSRVKPIV
  TPRFSLSCSETLMGELGNIAKTRDLHIQSHISENRDEVEAVKNLYPSYKNYTSVYDKNNLLTNKTVMAHG
  CYLSAEELNVFHERGASIAHCPNSNLSLSSGFLNVLEVLKHEVKIGLGTDVAGGYSYSMLDAIRRAVMVS
  NILLINKVNEKSLTLKEVFRLATLGGSQALGLDGEIGNFEVGKEFDAILINPKASDSPIDLFYGDFFGDI
  SEAVIQKFLYLGDDRNIEEVYVGGKQVVPFSSSV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_004284
• RefSeq Size: 5430
• RefSeq ORF: 1362
• Synonyms: CYPIN; GAH; GUANASE; NEDASIN
• Locus ID: 9615
• UniProt ID: Q9Y2T3, A0A024R231
• Cytogenetics: 9q21.13
• Summary: This gene encodes an enzyme responsible for the hydrolytic deamination of guanine. Studies in rat ortholog suggest this gene plays a role in microtubule assembly. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]
• Protein Pathways: Metabolic pathways, Purine metabolism