LRRC50 (DNAAF1) (NM_178452) Human Mass Spec Standard

CAT#: PH305452

LRRC50 MS Standard C13 and N15-labeled recombinant protein (NP_848547)

Product Images

Coomassie blue staining of purified DNAAF1 protein (Cat# TP305452). The protein was produced from HEK293T cells transfected with DNAAF1 cDNA clone (Cat# RC205452) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC205452
• Predicted MW: 80 kDa
• Protein Sequence:
  Sequence Link

  >RC205452 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MHPEPSEPATGGAAELDCAQEPGVEESAGDHGSAGRGGCKEEINDPKEICVGSSDTSYHSQQKQSGDNGS
  GGHFAHPREDREDRGPRMTKSSLQKLCKQHKLYITPALNDTLYLHFKGFDRIENLEEYTGLRCLWLQSNG
  IQKIENLEAQTELRCLFLQMNLLRKIENLEPLQKLDALNLSNNYIKTIENLSCLPVLNTLQMAHNHLETV
  EDIQHLQECLRLCVLDLSHNKLSDPEILSILESMPDLRVLNLMGNPVIRQIPNYRRTVTVRLKHLTYLDD
  RPVFPKDRACAEAWARGGYAAEKEERQQWESRERKKITDSIEALAMIKQRAEERKRQRESQERGEMTSSD
  DGENVPASAEGKEEPPGDRETRQKMELFVKESFEAKDELCPERPSGEEPPVEAKREDGGPEPEGTLPAET
  LLLSSPVEVKGEDGDGEPEGTLPAEAPPPPPPVEVKGEDGDQEPEGTLPAETLLLSPPVKVKGEDGDREP
  EGTLPAEAPPPLPLGAAREEPTPQAVATEGVFVTELDGTRTEDLETIRLETKETCCIDDLPDLEDDDETG
  KSLEDQNMCFPKIEVISSLSDDSDPELDYTSLPVLENLPTDTLSNIFAVSKDTSKAARVPFTDIFKKEAK
  RDSEIRKQDTKSPRPLIQELSDEDPSGQPLMPPTCQRDAAPLTSTGDRDSDFLAASSPVPTESAATPPET
  CVGVAQPSQALPTWDLTAFPAPKAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_848547
• RefSeq Size: 2451
• RefSeq ORF: 2175
• Synonyms: CILD13; DAU1; LRRC50; ODA7; swt
• Locus ID: 123872
• UniProt ID: Q8NEP3, A0A140VJN4
• Cytogenetics: 16q24.1
• Summary: The protein encoded by this gene is cilium-specific and is required for the stability of the ciliary architecture. It is involved in the regulation of microtubule-based cilia and actin-based brush border microvilli. Mutations in this gene are associated with primary ciliary dyskinesia-13. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]