HBA-T2 (HBB) (NM_000518) Human Mass Spec Standard

CAT#: PH303258

HBB MS Standard C13 and N15-labeled recombinant protein (NP_000509)

Product Images

Coomassie blue staining of purified HBB protein (Cat# TP303258). The protein was produced from HEK293T cells transfected with HBB cDNA clone (Cat# RC203258) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Tag: C-Myc/DDK
• Species: Human
• Expression Host: HEK293
• Expression cDNA Clone or AA Sequence: RC203258
• Predicted MW: 16 kDa
• Protein Sequence:
  Sequence Link

  >RC203258 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLG
  AFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVAN
  ALAHKYH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Concentration: >0.05 µg/µL as determined by microplate BCA method
• Labeling Method: Labeled with [U- 13C6, 15N4]-L-Arginine and [U- 13C6, 15N2]-L-Lysine
• Buffer: 25 mM Tris-HCl, 100 mM glycine, pH 7.3
• Storage: Store at -80°C. Avoid repeated freeze-thaw cycles.
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.

Reference Data

• RefSeq: NP_000509
• RefSeq Size: 626
• RefSeq ORF: 441
• Synonyms: beta-globin; CD113t-C; ECYT6
• Locus ID: 3043
• UniProt ID: P68871, D9YZU5
• Cytogenetics: 11p15.4
• Summary: The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult hemoglobin, Hb A. The normal adult hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta-zero-thalassemia. Reduced amounts of detectable beta globin causes beta-plus-thalassemia. The order of the genes in the beta-globin cluster is 5'-epsilon -- gamma-G -- gamma-A -- delta -- beta--3'. [provided by RefSeq, Jul 2008]