BTF (BCLAF1) Human Gene Knockout Kit (CRISPR)

CAT#: KN215839RB

BCLAF1 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: BTF
• Locus ID: 9774
• Components:

  KN215839G1, BTF gRNA vector 1 in pCas-Guide CRISPR vector

  KN215839G2, BTF gRNA vector 2 in pCas-Guide CRISPR vector

  KN215839RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001077440, NM_001077441, NM_001301038, NM_014739, NM_001363659
• UniProt ID: Q9NYF8
• Synonyms: bK211L9.1; BTF
• Summary: This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]