BTF (BCLAF1) (NM_014739) Human Tagged ORF Clone
CAT#: RC224372
BCLAF1 (Myc-DDK-tagged)-Human BCL2-associated transcription factor 1 (BCLAF1), transcript variant 1
ORF Plasmid: tGFP
Lentiviral Particles: DDK DDK w/ Puro mGFP mGFP w/ Puro
"NM_014739" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | BTF |
Synonyms | bK211L9.1; BTF |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC224372 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCGCTCCAATTCTAGATCACATTCTTCAAGGTCAAAGTCTAGATCACAGTCTAGTTCTCGATCAA GATCAAGATCTCATTCTAGAAAGAAGCGATACAGTTCTAGGTCTCGTTCCAGAACATATTCAAGGTCTCG TAGTAGAGATCGTATGTATTCTAGAGATTATCGTCGCGATTACAGAAATAATAGAGGAATGAGACGACCT TATGGGTACAGAGGAAGGGGTAGAGGGTATTATCAAGGAGGAGGAGGTAGATATCATCGAGGTGGTTATA GACCTGTCTGGAATAGAAGGCACTCTAGGAGTCCTAGACGAGGTCGTTCACGTTCCAGGAGTCCAAAAAG AAGATCCGTTTCTTCTCAAAGATCCAGAAGCAGATCTCGCCGGTCATATAGATCTTCTAGGTCTCCAAGA TCATCCTCTTCTCGTTCTTCATCCCCATATAGCAAATCTCCTGTTTCTAAAAGACGAGGGTCTCAGGAAA AACAAACCAAAAAAGCTGAAGGGGAACCCCAAGAAGAGAGTCCGTTGAAAAGTAAATCACAGGAGGAACC GAAAGATACATTTGAACATGACCCATCTGAGTCTATCGATGAATTTAATAAGTCATCAGCCACATCCGGT GATATTTGGCCTGGCCTTTCAGCTTATGATAATAGTCCTAGATCACCCCATAGTCCTTCACCTATTGCTA CACCACCTAGTCAGAGTTCATCTTGCTCTGATGCTCCCATGCTCAGTACAGTTCACTCTGCAAAAAATAC TCCTTCTCAGCATTCACATTCCATTCAGCATAGTCCTGAAAGGTCTGGGTCTGGTTCTGTTGGAAATGGA TCTAGTCGATACAGTCCTTCTCAGAATAGTCCAATTCATCACATCCCTTCACGAAGAAGTCCTGCAAAGA CAATCGCACCACAGAATGCTCCAAGAGATGAGTCTAGGGGCCGTTCCTCGTTTTATCCTGATGGTGGAGA TCAGGAAACTGCAAAGACTGGGAAGTTCTTAAAAAGGTTCACAGATGAAGAGTCTAGAGTATTCCTGCTT GATAGGGGTAATACCAGGGATAAAGAGGCTTCAAAAGAGAAAGGATCAGAGAAAGGGAGGGCAGAGGGAG AATGGGAAGATCAGGAAGCTCTAGATTACTTCAGTGATAAAGAGTCTGGAAAACAAAAGTTTAATGATTC AGAAGGGGATGACACAGAGGAGACAGAGGATTATAGACAGTTCAGGAAGTCAGTCCTCGCAGATCAGGGT AAAAGTTTTGCTACTGCATCTCACCGGAATACTGAGGAGGAAGGACTCAAGTACAAGTCCAAAGTTTCAC TGAAAGGCAATAGAGAAAGTGATGGATTTAGAGAAGAAAAAAATTATAAACTTAAAGAGACTGGATATGT AGTGGAAAGGCCTAGCACTACAAAAGATAAGCACAAAGAAGAAGACAAAAATTCTGAAAGAATAACAGTA AAGAAAGAAACTCAGTCACCTGAGCAGGTAAAGTCTGAAAAGCTCAAAGACCTCTTTGATTACAGTCCCC CTCTACACAAGAATCTGGATGCACGAGAAAAGTCTACCTTCAGAGAGGAAAGCCCACTTAGGATCAAAAT GATAGCGAGTGATTCTCACCGTCCTGAAGTCAAACTCAAAATGGCACCTGTTCCTCTTGATGATTCTAAC AGACCTGCTTCCTTGACTAAAGACAGGCTGCTTGCTAGTACACTTGTCCATTCTGTCAAGAAGGAGCAAG AATTCCGATCCATCTTTGACCACATTAAGTTGCCACAGGCCAGCAAAAGCACTTCAGAGTCATTTATTCA ACACATTGTGTCCTTGGTTCATCATGTTAAAGAGCAATACTTCAAGTCAGCTGCAATGACCCTAAACGAG CGGTTCACTTCGTATCAGAAAGCCACTGAAGAACATAGTACTCGGCAAAAGAGCCCTGAAATACACAGGA GAATTGACATCTCACCAAGTACCCTGAGGAAGCATACCCGTTTAGCAGGGGAAGAGAGAGTTTTTAAAGA AGAAAATCAAAAGGGAGATAAAAAATTAAGGTGTGACTCTGCTGACCTTCGGCATGACATTGATCGCCGT AGAAAAGAAAGAAGTAAAGAACGGGGAGATTCCAAGGGCTCCAGGGAATCCAGTGGATCAAGAAAGCAGG AAAAAACTCCAAAAGATTACAAGGAATACAAATCTTACAAAGATGACAGTAAACATAAAAGAGAGCAAGA TCATTCTCGATCTTCATCCTCTTCAGCATCACCTTCTTCTCCCAGTTCTCGAGAAGAAAAGGAGAGTAAG AAGGAAAGAGAAGAAGAATTTAAAACTCACCATGAAATGAAAGAATACTCAGGCTTTGCAGGAGTTAGCC GACCACGAGGAACCTTTTTTCGAATTAGAGGCAGAGGAAGAGCCAGAGGAGTTTTTGCTGGGACAAATAC TGGTCCAAACAACTCAAATACTACTTTTCAAAAGAGACCGAAGGAAGAGGAATGGGATCCAGAATATACC CCAAAGAGCAAGAAGTACTTCTTGCATGACGACAGAGATGATGGTGTGGATTATTGGGCCAAAAGAGGAA GAGGTCGTGGTACTTTTCAACGTGGCAGAGGGCGCTTTAACTTCAAAAAATCAGGTAGCAGTCCTAAATG GACTCATGACAAATACCAAGGGGATGGGATTGTTGAAGATGAAGAAGAGACCATGGAAAATAATGAAGAA AAGAAGGACAGACGCAAGGAAGAAAAGGAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224372 protein sequence
Red=Cloning site Green=Tags(s) MGRSNSRSHSSRSKSRSQSSSRSRSRSHSRKKRYSSRSRSRTYSRSRSRDRMYSRDYRRDYRNNRGMRRP YGYRGRGRGYYQGGGGRYHRGGYRPVWNRRHSRSPRRGRSRSRSPKRRSVSSQRSRSRSRRSYRSSRSPR SSSSRSSSPYSKSPVSKRRGSQEKQTKKAEGEPQEESPLKSKSQEEPKDTFEHDPSESIDEFNKSSATSG DIWPGLSAYDNSPRSPHSPSPIATPPSQSSSCSDAPMLSTVHSAKNTPSQHSHSIQHSPERSGSGSVGNG SSRYSPSQNSPIHHIPSRRSPAKTIAPQNAPRDESRGRSSFYPDGGDQETAKTGKFLKRFTDEESRVFLL DRGNTRDKEASKEKGSEKGRAEGEWEDQEALDYFSDKESGKQKFNDSEGDDTEETEDYRQFRKSVLADQG KSFATASHRNTEEEGLKYKSKVSLKGNRESDGFREEKNYKLKETGYVVERPSTTKDKHKEEDKNSERITV KKETQSPEQVKSEKLKDLFDYSPPLHKNLDAREKSTFREESPLRIKMIASDSHRPEVKLKMAPVPLDDSN RPASLTKDRLLASTLVHSVKKEQEFRSIFDHIKLPQASKSTSESFIQHIVSLVHHVKEQYFKSAAMTLNE RFTSYQKATEEHSTRQKSPEIHRRIDISPSTLRKHTRLAGEERVFKEENQKGDKKLRCDSADLRHDIDRR RKERSKERGDSKGSRESSGSRKQEKTPKDYKEYKSYKDDSKHKREQDHSRSSSSSASPSSPSSREEKESK KEREEEFKTHHEMKEYSGFAGVSRPRGTFFRIRGRGRARGVFAGTNTGPNNSNTTFQKRPKEEEWDPEYT PKSKKYFLHDDRDDGVDYWAKRGRGRGTFQRGRGRFNFKKSGSSPKWTHDKYQGDGIVEDEEETMENNEE KKDRRKEEKE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_014739 |
ORF Size | 2760 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_014739.3 |
RefSeq Size | 7277 bp |
RefSeq ORF | 2763 bp |
Locus ID | 9774 |
UniProt ID | Q9NYF8 |
Cytogenetics | 6q23.3 |
Protein Families | Transcription Factors |
MW | 106.1 kDa |
Gene Summary | This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC224372L1 | Lenti ORF clone of Human BCL2-associated transcription factor 1 (BCLAF1), transcript variant 1, Myc-DDK-tagged |
USD 1,142.00 |
|
RC224372L2 | Lenti ORF clone of Human BCL2-associated transcription factor 1 (BCLAF1), transcript variant 1, mGFP tagged |
USD 1,142.00 |
|
RC224372L3 | Lenti ORF clone of Human BCL2-associated transcription factor 1 (BCLAF1), transcript variant 1, Myc-DDK-tagged |
USD 1,142.00 |
|
RC224372L4 | Lenti ORF clone of Human BCL2-associated transcription factor 1 (BCLAF1), transcript variant 1, mGFP tagged |
USD 1,142.00 |
|
RG224372 | BCLAF1 (tGFP-tagged) - Human BCL2-associated transcription factor 1 (BCLAF1), transcript variant 1 |
USD 1,042.00 |
|
SC114841 | BCLAF1 (untagged)-Human BCL2-associated transcription factor 1 (BCLAF1), transcript variant 1 |
USD 843.00 |
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