Tuberin (TSC2) Human Gene Knockout Kit (CRISPR)

CAT#: KN211461LP

TSC2 - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 Luciferase-Puro donor, 1 scramble control
• Donor DNA: Luciferase-Puro
• Symbol: Tuberin
• Locus ID: 7249
• Components:

  KN211461G1, Tuberin gRNA vector 1 in pCas-Guide CRISPR vector

  KN211461G2, Tuberin gRNA vector 2 in pCas-Guide CRISPR vector

  KN211461LPD, donor DNA containing left and right homologous arms and Luciferase-Puro functional cassette.

  Homologous arm and Luciferase-Puro sequences:

  pUC vector backbone in gray; Left arm sequence in blue; Luciferase-Puro in green; Right arm in violet

  ACGGGGCAGC GGCCTAGAGA GGCGGACCCC GCAGTGTCCG GGTCCCGGGC CCTCACCCGC GCCCACTGCA ACCCGACTCC GGAGCTCCGA GCATCCCTTA GTTTTAAGTC ATGGCGGGTG CGAACGGGTC TCTGCTGCAG GCGGCTCCGT GACAGCTCCT GCTTCACATG GGTAGAGGAG AGACGGCAAA CGTCGGGGCT CCCAGGACTT CGCGGCGGCA GTCCTAACCC GTGCACTGAG TCGGGCGGGG CGGGCGGCAG CGTCTGAGTA GAGAGCTCTC TAGACCAGGC CTGGTGTCTC CCGGGCTTTC CTAGGTGCCT GTTTGCGAGC TGGTCAGCTG GGCTGTAGTT GAGTTCTCCC AGGGAGTGTG GGAGGAAAGG TTATGCCCAC CAGAGACCCA GGGTCCTGAC GGCTGGAGGT CCGCAGTGGG GAAGGTGGGC AGAGGTGTTG CTCAGATGTC CCCATTCCTG TTTCGTTTGC ACAGAGGGGT TTTCTGGTGC GTCCTGGTCC ACCTGCAGAG GGTAAACAGA CGGAGTTTAT CATCACCGCG GAAATACTGA GAGTGAGTGA GCTACCTGTG TCTTTGCTAG GCTAGAGGGA AATGCAGAGA AGGCTGGGTT TGGTCTTGCA CCAGGTTCTG TGGGAGACGG GTTGCTGGCA ACTGTCTACA CAGGAATGCT GTCTCCAGTA CTTGGAGGCC GACATGTCCT TCCTCAGGAG ACTTCGAAAG TCAGGATCTT GGTGACCTGG TTCAGGCACA CTTGAGTTAC TATTCAGATG TTGATGAAAG AAAGCCCATC TTATAGATGA GGCCTGAGTA TCATCATCTT CACAGGTAAG GATATGTCCA GGTGTTACAT GATAAGATGT GAGTTCCTGG GAGAAGTAGG TTCTTTATTT TATTTTTTTT TTGAGACTGA GTCTTGCTCT GTTGCCTAGG CTGGAGTGCA GTGGCACAAT CTCTGCTCGC TGCTACCTCT GCCTCCCAGT TTCAAGTGGT TCTCCTGCCT CAGCCTCCTG AGTAGCTGGG ACTACAGACA CGTGCCACCA CACCCATTTA ATTTTTGTAT TTTTAGTAGA GATGGAGTTT CACTATCTTG GCCAGGCTGG TCT

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_000548, NM_001077183, NM_001114382, NM_001318827, NM_001318829, NM_001318831, NM_001318832, NM_021055, NM_021056, NM_001363528, NM_001370405, NM_001370404
• UniProt ID: P49815
• Synonyms: LAM; PPP1R160; TSC4
• Summary: Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]