Mouse Lig4 activation kit by CRISPRa

CAT#: GA216310

Lig4 CRISPRa kit - CRISPR gene activation of mouse ligase IV, DNA, ATP-dependent

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Lig4
• Locus ID: 319583
• Kit Components:

  GA216310G1, Lig4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA216310G2, Lig4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA216310G3, Lig4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_176953
• UniProt ID: Q8BTF7
• Synonyms: 5830471N16Rik; tiny
• Summary: Efficiently joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends.[UniProtKB/Swiss-Prot Function]