Mouse Arhgef4 activation kit by CRISPRa

CAT#: GA213900

Arhgef4 CRISPRa kit - CRISPR gene activation of mouse Rho guanine nucleotide exchange factor (GEF) 4

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Arhgef4
• Locus ID: 226970
• Kit Components:

  GA213900G1, Arhgef4 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA213900G2, Arhgef4 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA213900G3, Arhgef4 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_183019, NM_001368773
• UniProt ID: Q7TNR9
• Synonyms: 9330140K16Rik; Asef; ENSMUSG00000070955
• Summary: Acts as guanine nucleotide exchange factor (GEF) for RHOA, RAC1 and CDC42 GTPases. Binding of APC may activate RAC1 GEF activity. The APC-ARHGEF4 complex seems to be involved in cell migration as well as in E-cadherin-mediated cell-cell adhesion (By similarity). Required for MMP9 up-regulation via the JNK signaling pathway in colorectal tumor cells. Involved in tumor angiogenesis and may play a role in intestinal adenoma formation and tumor progression.[UniProtKB/Swiss-Prot Function]