Mouse Sgms1 activation kit by CRISPRa

CAT#: GA212868

Sgms1 CRISPRa kit - CRISPR gene activation of mouse sphingomyelin synthase 1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Sgms1
• Locus ID: 208449
• Kit Components:

  GA212868G1, Sgms1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA212868G2, Sgms1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA212868G3, Sgms1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001168525, NM_001168526, NM_144792, NM_001362423
• UniProt ID: Q8VCQ6
• Synonyms: 9530058O11Rik; AI841905; C80702; Mob; Sms1; Sor1; Tmem23
• Summary: Sphingomyelin synthases synthesize the sphingolipid, sphingomyelin, through transfer of the phosphatidyl head group, phosphatidylcholine, on to the primary hydroxyl of ceramide. The reaction is bidirectional depending on the respective levels of the sphingolipid and ceramide. Golgi apparatus SMS1 directly and specifically recognizes the choline head group on the substrate, requiring two fatty chains on the choline-P donor molecule in order to be recognized efficiently as a substrate. Major form in macrophages. Required for cell growth in certain cell types (By similarity). Suppresses BAX-mediated apoptosis and also prevents cell death in response to stimuli such as hydrogen peroxide, osmotic stress, elevated temperature and exogenously supplied sphingolipids. May protect against cell death by reversing the stress-inducible increase in levels of proapoptotic ceramide.[UniProtKB/Swiss-Prot Function]