Mouse Amer1 activation kit by CRISPRa

CAT#: GA209211

Amer1 CRISPRa kit - CRISPR gene activation of mouse APC membrane recruitment 1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Amer1
• Locus ID: 72345
• Kit Components:

  GA209211G1, Amer1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA209211G2, Amer1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA209211G3, Amer1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_175179
• UniProt ID: Q7TS75
• Synonyms: 2810002O09Rik; AW492303; Fam123b; Wtx
• Summary: Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta-catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development (By similarity).[UniProtKB/Swiss-Prot Function]