Mouse Spdya activation kit by CRISPRa

CAT#: GA208656

Spdya CRISPRa kit - CRISPR gene activation of mouse speedy/RINGO cell cycle regulator family, member A

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Spdya
• Locus ID: 70891
• Kit Components:

  GA208656G1, Spdya gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA208656G2, Spdya gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA208656G3, Spdya gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001142631, NM_029254
• UniProt ID: Q5IBH7
• Synonyms: 4921517J08Rik; 4930548B21Rik; GS4; MLZ-465; Spdy1
• Summary: Regulates the G1/S phase transition of the cell cycle by binding and activating CDK1 and CDK2 (PubMed:15611625). Contributes to CDK2 activation without promoting CDK2 phosphorylation, by inducing a conformation change of the CDK2 T-loop that obstructs the substrate-binding cleft prior to kinase activation. Interferes with CDKN1B-mediated inhibition of CDK2. Mediates cell survival during the DNA damage process through activation of CDK2 (By similarity).[UniProtKB/Swiss-Prot Function]