Mouse Mob1b activation kit by CRISPRa

CAT#: GA207823

Mob1b CRISPRa kit - CRISPR gene activation of mouse MOB kinase activator 1B

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Mob1b
• Locus ID: 68473
• Kit Components:

  GA207823G1, Mob1b gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA207823G2, Mob1b gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA207823G3, Mob1b gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_026735
• UniProt ID: Q8BPB0
• Synonyms: 1110003E08Rik; AU015450; B230364F10; Mobkl1a
• Summary: Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38L (By similarity).[UniProtKB/Swiss-Prot Function]