Mouse Psmd10 activation kit by CRISPRa

CAT#: GA205564

Psmd10 CRISPRa kit - CRISPR gene activation of mouse proteasome (prosome, macropain) 26S subunit, non-ATPase, 10

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Psmd10
• Locus ID: 53380
• Kit Components:

  GA205564G1, Psmd10 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA205564G2, Psmd10 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA205564G3, Psmd10 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001164177, NM_016883
• UniProt ID: Q9Z2X2
• Synonyms: AW554874
• Summary: Acts as a chaperone during the assembly of the 26S proteasome, specifically of the PA700/19S regulatory complex (RC). In the initial step of the base subcomplex assembly is part of an intermediate PSMD10:PSMC4:PSMC5:PAAF1 module which probably assembles with a PSMD5:PSMC2:PSMC1:PSMD2 module (By similarity). Independently of the proteasome, regulates EGF-induced AKT activation through inhibition of the RHOA/ROCK/PTEN pathway, leading to prolonged AKT activation. Plays an important role in RAS-induced tumorigenesis.[UniProtKB/Swiss-Prot Function]