Mouse Nck1 activation kit by CRISPRa

CAT#: GA202840

Nck1 CRISPRa kit - CRISPR gene activation of mouse non-catalytic region of tyrosine kinase adaptor protein 1

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: Nck1
• Locus ID: 17973
• Kit Components:

  GA202840G1, Nck1 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA202840G2, Nck1 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA202840G3, Nck1 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001324530, NM_010878
• UniProt ID: Q99M51
• Synonyms: 6330586M15Rik; D230010O13Rik; Nck; Nck-1
• Summary: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR, but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by dsRNA (By similarity).[UniProtKB/Swiss-Prot Function]