Human HSPC152 (TRMT112) activation kit by CRISPRa

CAT#: GA109840

TRMT112 CRISPRa kit - CRISPR gene activation of human tRNA methyltransferase subunit 11-2

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: TRMT112
• Locus ID: 51504
• Kit Components:

  GA109840G1, HSPC152 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA109840G2, HSPC152 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA109840G3, HSPC152 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001286082, NM_001286084, NM_016404
• UniProt ID: Q9UI30
• Synonyms: HSPC152; HSPC170; hTrm112; TRM112; TRMT11-2
• Summary: Acts as an activator of both rRNA/tRNA and protein methyltransferases (PubMed:25851604). Together with methyltransferase BUD23, methylates the N(7) position of a guanine in 18S rRNA (PubMed:25851604). The heterodimer with HEMK2/N6AMT1 catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor (PubMed:18539146). The heterodimer with ALKBH8 catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA species (PubMed:20308323). Involved in the pre-rRNA processing steps leading to small-subunit rRNA production (PubMed:25851604).[UniProtKB/Swiss-Prot Function]