Human RAD17 activation kit by CRISPRa

CAT#: GA103997

RAD17 CRISPRa kit - CRISPR gene activation of human RAD17 checkpoint clamp loader component

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: RAD17
• Locus ID: 5884
• Kit Components:

  GA103997G1, RAD17 gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103997G2, RAD17 gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103997G3, RAD17 gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001278622, NM_002873, NM_133338, NM_133339, NM_133340, NM_133341, NM_133342, NM_133343, NM_133344
• UniProt ID: O75943
• Synonyms: CCYC; HRAD17; R24L; RAD17SP; RAD24
• Summary: The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013]