Human PIGA activation kit by CRISPRa

CAT#: GA103546

PIGA CRISPRa kit - CRISPR gene activation of human phosphatidylinositol glycan anchor biosynthesis class A

Product Images

Specifications

Product Data

• Format: 3 gRNAs (5ug each), 1 scramble ctrl (10ug) and 1 enhancer vector (10ug)
• Symbol: PIGA
• Locus ID: 5277
• Kit Components:

  GA103546G1, PIGA gRNA vector 1 in pCas-Guide-GFP-CRISPRa

  GA103546G2, PIGA gRNA vector 2 in pCas-Guide-GFP-CRISPRa

  GA103546G3, PIGA gRNA vector 3 in pCas-Guide-GFP-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100077

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPRa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_002641, NM_020472, NM_020473, NR_033835, NR_033836
• UniProt ID: P37287
• Synonyms: GPI3; MCAHS2; PIG-A; PNH1
• Summary: This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12. [provided by RefSeq, Jun 2010]