hexon (AC_000005) Virus Tagged ORF Clone

CAT#: VC100233

Myc-DDK-tagged ORF clone of viral ORF for hexon [Human adenovirus A], codon optimized for human cell expression, AP_000121

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hexon
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC100233 represents NCBI reference of AP_000121 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTACACCGAGCATGATGCCCCAGTGGAGCTACATGCATATTGCGGGACAGGATGCAAGCGAATACC
  TCAGCCCCGGCCTCGTCCAATTCGCGCGAGCAACCGATACCTATTTCACACTGGGGAACAAGTTCCGAAA
  TCCTACAGTGGCGCCGACACACGATGTAACAACTGATCGGAGTCAGCGATTGACACTTCGATTCGTCCCC
  GTCGATCGGGAAGATACTACTTATTCCTATAAGGCGAGATTCACGCTGGCCGTGGGAGATAACAGAGTTC
  TTGATATGGCATCCTCCTATTTCGACATTAGAGGCGTTCTCGACAGAGGCCCCTCCTTCAAGCCCTATAG
  TGGAACCGCCTACAATAGCCTGGCCCCTAAGGGCGCCCCTAACGCCTCTCAGTGGTCCGACAATGCAAAA
  CTGAACACATTCGCCCAGGCCCCTTACTTGTCCGACACGATCACTGCAGCCGATGGAATCAAGGTGGGAA
  CAGACACCGCACAAGCAGGTGCCGCCGTTTACGCGAACAAGACTTACCAGCCAGAGCCACAAGTCGGGCC
  TTCCGAGTGGAACACCTCCATCGAGAACGTTAAGGCCGGGGGTCGCGCCCTGAAGCAGACAACTGCTATG
  CAGCCCTGCTATGGTTCCTACGCTAGGCCCACAAACGAGCACGGTGGGCAGTCTAAAGACGACAATATCG
  AGCTGAAATTCTTTGATAGTGCGAACAACGCTGCAAACACTGCCCAGGTCGTATTCTATACCGAGGATGT
  AAATTTGGAAATGCCAGACACACATCTCGTGTTTAAGCCTACGGTTACGAATGGCACCATAGCTAGTGAG
  TCTCTGCTGGGACAACAGGCTGCCCCAAATAGGGCCAACTACATCGCGTTTCGCGACAATTTCATTGGCC
  TGATGTACTACAATTCTACAGGCAATATGGGCGTGCTGGCTGGCCAGGCCAGCCAGCTGAACGCCGTTGT
  GGATCTTCAGGACCGCAATACTGAACTGTCCTATCAACTGATGCTTGACGCACTGGGCGATCGAACCAGG
  TACTTCTCTCTGTGGAACTCCGCTGTGGATTCATATGACCCCGATGTGCGCGTAATTGAGAATCATGGAG
  TTGAGGATGAACTGCCCAACTACTGCTTCCCCCTGTCCGCCGTCGGTGAAATAAAAAATTACAAGGGGAT
  TAAACCGGACAACGGAGGGGGGGGCGGCTGGACCGCAGATAATACAGTTTCCGAAGCGAATCACATCGGT
  ATAGGTAATATCGCTGCAATGGAGATAAATCTGCAGGCGAACCTGTGGCGGTCTTTCCTCTATAGCAACG
  TGGGCCTTTACCTTCCCGACGATTTGAAATATACTCCTGGGAATATAAAACTGCCCGATAACAAAAATAC
  CTACGAATACATGAATGGAAGAGTTACTGCTCCGGGCCTGGTGGACACTTATGTCAACATCGGCGCAAGG
  TGGTCCCCCGACGTGATGGACAACGTCAATCCCTTTAATCACCACAGGAATGCAGGACTCAGATATCGCT
  CTATGTTGCTTGGCAACGGGCGCTTCGTCCCGTTCCATATACAAGTTCCCCAGAAGTTCTTTGCTATACG
  AAACCTGCTGCTGTTGCCCGGCTCATACACCTACGAGTGGAATTTTCGAAAAGATGTCAATATGATTCTC
  CAGTCCACACTGGGCAACGACCTCAGGGTGGACGGTGCGTCAGTGCGCTTTGATAATATTGCCTTGTACG
  CCAACTTTTTTCCAATGGCCCATAATACTGCAAGTACACTGGAGGCCATGCTTAGGAATGACACAAACGA
  CCAAAGCTTCAACGACTACCTCTGCGCCGCCAATATGCTCTATCCGATTCCAGCCAACGCAACCAGCGTG
  CCCATAAGCATCCCCAGCAGGAACTGGGCTGCCTTTAGAGGTTGGTCCTTCACCCGCCTTAAAACTAAGG
  AAACTCCTTCTCTTGGAAGTGGCTTCGATCCTTACTTCGTTTACTCAGGAACCATTCCATATCTCGATGG
  GACATTCTACCTGAATCACACCTTCAAGAAAGTCTCCATCATGTTTGATTCTAGCGTGTCTTGGCCAGGG
  AACGACCGCCTTCTCACCCCGAACGAGTTCGAAATCAAAAGATCCGTTGACGGAGAGGGCTACAACGTCG
  CCCAGTGCAATATGACTAAGGATTGGTTCTTGATTCAGATGTTGAGCCACTATAATATTGGGTACCAAGG
  GTTTTATATACCTGAGAGCTACAAAGACAGAATGTACAGCTTTTTCAGAAACTTCCAGCCTATGTCCAGG
  CAAGTGGTTGACACTACTGAATATAAAAACTACAAGAAAGTCACTGTGGAGTTCCAACACAATAATAGTG
  GATTCGTGGGCTATCTCGGCCCTACCATGAGAGAAGGTCAGGCATATCCTGCTAATTATCCTTATCCTCT
  GATTGGACAGACTGCGGTGGAAAGCATTACACAGAAGAAATTTCTGTGCGATCGGGTTATGTGGAGGATT
  CCCTTTTCCTCCAATTTTATGTCTATGGGTGCTTTGACAGATCTGGGGCAGAACATGTTGTATGCTAATA
  GTGCCCACGCCCTCGACATGACGTTCGAGGTGGATCCTATGGACGAGCCCACACTCTTGTATGTTCTTTT
  CGAAGTTTTCGACGTAGTGAGGATCCACCAGCCACACCGCGGTGTAATTGAGGCAGTTTATCTGAGAACG
  CCCTTCAGCGCTGGCAACGCGACGACG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC100233 representing AP_000121
  Red=Cloning sites Green=Tags
  
  MATPSMMPQWSYMHIAGQDASEYLSPGLVQFARATDTYFTLGNKFRNPTVAPTHDVTTDRSQRLTLRFVP
  VDREDTTYSYKARFTLAVGDNRVLDMASSYFDIRGVLDRGPSFKPYSGTAYNSLAPKGAPNASQWSDNAK
  LNTFAQAPYLSDTITAADGIKVGTDTAQAGAAVYANKTYQPEPQVGPSEWNTSIENVKAGGRALKQTTAM
  QPCYGSYARPTNEHGGQSKDDNIELKFFDSANNAANTAQVVFYTEDVNLEMPDTHLVFKPTVTNGTIASE
  SLLGQQAAPNRANYIAFRDNFIGLMYYNSTGNMGVLAGQASQLNAVVDLQDRNTELSYQLMLDALGDRTR
  YFSLWNSAVDSYDPDVRVIENHGVEDELPNYCFPLSAVGEIKNYKGIKPDNGGGGGWTADNTVSEANHIG
  IGNIAAMEINLQANLWRSFLYSNVGLYLPDDLKYTPGNIKLPDNKNTYEYMNGRVTAPGLVDTYVNIGAR
  WSPDVMDNVNPFNHHRNAGLRYRSMLLGNGRFVPFHIQVPQKFFAIRNLLLLPGSYTYEWNFRKDVNMIL
  QSTLGNDLRVDGASVRFDNIALYANFFPMAHNTASTLEAMLRNDTNDQSFNDYLCAANMLYPIPANATSV
  PISIPSRNWAAFRGWSFTRLKTKETPSLGSGFDPYFVYSGTIPYLDGTFYLNHTFKKVSIMFDSSVSWPG
  NDRLLTPNEFEIKRSVDGEGYNVAQCNMTKDWFLIQMLSHYNIGYQGFYIPESYKDRMYSFFRNFQPMSR
  QVVDTTEYKNYKKVTVEFQHNNSGFVGYLGPTMREGQAYPANYPYPLIGQTAVESITQKKFLCDRVMWRI
  PFSSNFMSMGALTDLGQNMLYANSAHALDMTFEVDPMDEPTLLYVLFEVFDVVRIHQPHRGVIEAVYLRT
  PFSAGNATT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: AC_000005
• ORF Size: 2757 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: AC_000005.1, AP_000121
• RefSeq ORF: 2757 bp
• MW: 103.0 kDa