GAPex 5 (GAPVD1) (NM_001282679) Human Untagged Clone

CAT#: SC338054

GAPVD1 (untagged) - Human GTPase activating protein and VPS9 domains 1 (GAPVD1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GAPVD1
• Synonyms: GAPex-5; GAPEX5; RAP6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001282679, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGAAACTAGATATTCATACTCTGGCTCATCACCTCAAGCAGGAACGCTTATATGTAAACTCTGAGA
  AACAGCTCATTCAGAGGCTCAATGCAGATGTACTTAAGACAGCTGAAAAGTTGTATCGTACAGCATGGAT
  TGCGAAGCAACAGAGAATCAATTTGGATCGGCTTATCATAACCAGTGCTGAAGCTTCCCCTGCTGAATGT
  TGCCAACATGCCAAAATTTTGGAAGATACACAATTTGTTGATGGGTATAAGCAATTGGGATTTCAGGAGA
  CTGCTTATGGAGAATTCTTGAGTCGATTGAGGGAAAATCCTCGTCTTATTGCCTCCTCTTTGGTTGCTGG
  AGAGAAACTTAATCAGGAGAACACACAAAGTGTTATTTACACAGTTTTTACCTCCCTGTATGGCAATTGC
  ATCATGCAAGAAGATGAAAGCTACCTCCTTCAGGTTTTGCGATACTTGATTGAATTTGAACTTAAAGAAA
  GTGACAACCCTAGGCGACTTTTGAGGAGAGGAACTTGTGCCTTCAGCATCTTATTTAAACTTTTTTCTGA
  AGGACTGTTTTCTGCCAAACTTTTCCTCACAGCCACTTTACATGAGCCAATTATGCAACTGCTTGTTGAA
  GATGAAGATCACCTGGAAACAGATCCAAACAAGCTAATTGAGAGGTTCTCTCCATCTCAGCAGGAAAAAC
  TCTTTGGAGAGAAGGGCTCAGATAGATTCAGGCAAAAAGTTCAAGAAATGGTGGAGTCCAATGAAGCAAA
  GCTAGTGGCTTTGGTGAACAAATTTATTGGTTATCTCAAACAGAACACATATTGTTTTCCACATAGTTTA
  AGGTGGATCGTGTCTCAGATGTACAAAACCCTCTCCTGTGTAGATAGGCTGGAAGTTGGGGAGGTCAGGG
  CAATGTGTACTGATCTCCTGTTGGCCTGCTTCATTTGTCCTGCAGTTGTCAATCCAGAACAATATGGAAT
  AATTTCCGATGCTCCTATTAATGAAGTAGCACGATTTAATCTGATGCAGGTAGGCCGCCTTTTGCAGCAG
  TTAGCAATGACTGGCTCTGAAGAGGGAGATCCCCGAACAAAGAGCAGCCTTGGAAAGTTTGACAAAAGCT
  GTGTTGCCGCTTTCCTTGATGTTGTGATTGGGGGCCGTGCAGTGGAGACCCCTCCATTGTCTTCCGTCAA
  TCTTCTGGAAGGATTGAGCAGAACTGTGGTTTATATAACCTACAGTCAGCTTATTACTCTGGTGAATTTT
  ATGAAGAGTGTGATGTCTGGAGATCAACTGAGAGAAGATAGAATGGCTCTTGACAATTTATTGGCAAACC
  TACCCCCGGCCAAGCCAGGAAAAAGTAGCAGTTTAGAAATGACTCCCTACAATACACCTCAGCTATCTCC
  AGCAACCACTCCAGCAAATAAAAAGAATCGATTACCTATAGCAACTCGGAGCAGAAGCCGCACCAATATG
  CTAATGGACCTACATATGGACCATGAAGGATCATCTCAAGAAACCATCCAGGAGGTGCAACCAGAAGAGG
  TGTTGGTCATTTCCTTAGGTACAGGTCCCCAGCTTACTCCAGGGATGATGTCAGAAAATGAGGTCCTAAA
  CATGCAGCTTTCGGATGGAGGACAAGGAGATGTCCCTGTTGATGAAAACAAACTCCATGGTAAACCTGAT
  AAAACCTTGCGCTTTTCCCTCTGCAGTGATAATCTGGAAGGAATATCTGAAGGTCCTTCAAATCGCTCCA
  ATTCAGTGTCCTCCCTAGACCTAGAAGGAGAGTCTGTGTCAGAACTTGGAGCAGGACCTTCTGGCAGTAA
  TGGAGTTGAAGCTCTACAGCTGTTAGAACATGAGCAAGCTACAACACAGGATAACCTTGATGATAAGCTA
  AGGAAGTTTGAAATTCGTGACATGATGGGATTAACAGATGATAGGGACATATCAGAAACAGTGAGTGAGA
  CCTGGAGTACAGACGTCTTGGGAAGTGACTTTGACCCTAATATTGATGAAGATCGCTTGCAAGAAATTGC
  AGGTGCTGCAGCAGAGAACATGTTAGGCAGTTTGCTGTGCCTCCCAGGTTCAGGGTCAGTGCTTCTTGAC
  CCCTGCACTGGTTCTACCATATCAGAGACAACAAGTGAAGCTTGGAGTGTAGAGGTATTGCCAAGTGACT
  CAGAGGCCCCAGACCTAAAGCAGGAGGAGCGTCTGCAAGAACTGGAGAGCTGTTCTGGACTGGGTAGCAC
  ATCTGATGATACGGATGTCAGGGAGGTCAGTTCCCGCCCCAGCACACCAGGCCTCAGTGTTGTGTCCGGC
  ATAAGTGCAACCTCTGAGGATATTCCCAATAAGATTGAAGACCTGAGATCTGAGTGCAGCTCTGATTTTG
  GGGGTAAAGATTCTGTCACTAGTCCAGACATGGATGAAATAACTCACGGTGCCCACCAGCTGACCTCTCC
  TCCTTCTCAGTCAGAGTCTCTGCTGGCCATGTTTGATCCACTGTCTTCACATGAAGGGGCTTCTGCTGTG
  GTAAGGCCAAAGGTTCACTATGCTAGGCCATCGCATCCACCACCAGATCCCCCAATCCTGGAAGGAGCTG
  TGGGAGGAAATGAGGCCAGGTTGCCAAACTTTGGTTCCCATGTTTTAACTCCAGCTGAAATGGAGGCATT
  CAAGCAAAGGCATTCTTACCCTGAGAGACTAGTTCGAAGCAGGAGCTCTGATATAGTATCTTCTGTCCGG
  AGACCCATGAGTGACCCCAGCTGGAACCGGCGTCCAGGAAATGAAGAGCGAGAACTCCCTCCAGCTGCAG
  CCATTGGTGCTACTTCTTTGGTGGCTGCACCTCATTCATCATCTTCATCCCCGAGTAAGGACTCCTCAAG
  AGGAGAGACTGAAGAACGCAAAGATAGCGATGATGAGAAATCAGACAGGAACAGACCTTGGTGGAGAAAA
  CGTTTTGTTTCAGCCATGCCTAAAGCTCCTATACCATTTAGAAAGAAAGAAAAACAAGAAAAAGACAAAG
  ATGATCTGGGGCCTGACAGATTCTCAACACTCACAGATGATCCCAGCCCTAGACTCAGTGCACAAGCTCA
  GGTGGCTGAGGATATTCTGGACAAATACAGGAATGCCATTAAACGGACCAGCCCCAGTGATGGAGCAATG
  GCAAACTATGAAAGTACAGGTGATAATCATGACAGAGATTTGAGTAGTAAACTTTTATATCATAGTGATA
  AAGAGGTTATGGGTGATGGTGAAAGTGCACATGATTCTCCCCGTGACGAAGCACTGCAGAACATCTCGGC
  TGATGATCTCCCAGACTCTGCAAGCCAAGCAGCCCACCCGCAGGATTCAGCTTTCTCTTACAGAGATGCA
  AAAAAGAAACTGAGGCTTGCTCTTTGCTCTGCGGACTCTGTTGCCTTCCCAGTGCTGACCCATTCAACAA
  GGAATGGTTTACCAGACCACACAGACCCAGAAGACAATGAAATTGTATGCTTCTTAAAAGTTCAAATAGC
  TGAAGCAATTAATTTACAAGATAAGAATCTAATGGCTCAACTTCAAGAAACAATGCGCTGTGTGTGCCGT
  TTTGATAATAGGACTTGTAGGAAACTGCTGGCTTCGATTGCTGAGGACTACAGAAAAAGAGCCCCATATA
  TTGCTTATCTCACTCGTTGTCGACAAGGACTACAGACCACACAGGCTCACCTGGAAAGGCTATTGCAAAG
  AGTTTTGCGGGACAAAGAAGTGGCCAATCGATACTTTACCACTGTCTGTGTGAGATTACTGCTTGAGAGC
  AAAGAAAAGAAGATCAGGGAATTCATTCAAGACTTTCAGAAACTCACCGCAGCTGACGATAAAACTGCTC
  AGGTAGAAGATTTTCTGCAGTTTCTTTATGGTGCAATGGCCCAGGATGTCATATGGCAAAACGCGAGTGA
  AGAACAGCTTCAAGATGCACAGCTGGCCATTGAGCGAAGCGTGATGAACCGGATTTTCAAGCTCGCCTTC
  TACCCTAATCAAGATGGGGACATACTTCGCGACCAGGTTCTTCATGAACATATCCAGAGATTGTCTAAAG
  TAGTGACTGCAAATCACAGAGCTCTTCAGATACCAGAGGTTTATCTTCGAGAAGCACCATGGCCATCTGC
  ACAATCAGAAATCAGGACAATAAGTGCTTATAAAACCCCCCGGGACAAAGTGCAGTGCATCCTGAGAATG
  TGCTCTACGATTATGAACCTCCTGAGCCTGGCCAATGAGGACTCTGTCCCTGGAGCGGATGACTTTGTTC
  CTGTGTTGGTGTTTGTGTTGATAAAGGCAAATCCACCCTGTTTGCTGTCTACTGTGCAGTATATCAGTAG
  CTTTTATGCTAGCTGTCTGTCTGGAGAGGAGTCCTATTGGTGGATGCAGTTCACAGCAGCAGTAGAATTC
  ATTAAAACCATCGATGACCGAAAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282679
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282679.1, NP_001269608.1
• RefSeq Size: 7083 bp
• RefSeq ORF: 4437 bp
• Locus ID: 26130
• UniProt ID: Q14C86
• Cytogenetics: 9q33.3
• Gene Summary: Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras.[UniProtKB/Swiss-Prot Function]