NEDD4 (NM_001284338) Human Untagged Clone

CAT#: SC338000

NEDD4 (untagged) - Human neural precursor cell expressed, developmentally down-regulated 4, E3 ubiquitin protein ligase (NEDD4), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NEDD4
• Synonyms: NEDD4-1; RPF1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001284338, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCACAAAGCTTACGATTGCACTTTGCAGCCAGAAGAAGCAATACTTACCCTTTGTCAGAAACCTCCG
  GAGATGACTTGGATAGCCATGTTCACATGTGCTTCAAAAGACCAACACGGATTTCAACGTCTAACGTTGT
  TCAAATGAAGCTGACTCCCAGACAGACTGCACTAGCTCCGTTAATAAAGGAAAACGTTCAGTCTCAAGAA
  AGATCATCTGTTCCCTCATCTGAAAATGTTAATAAAAAGAGCAGCTGTCTACAGATTTCACTACAGCCAA
  CAAGGTACAGTGGATATCTTCAGTCTAGCAATGTCTTAGCTGATAGTGATGATGCTTCGTTTACTTGTAT
  CTTGAAGGATGGTATTTACAGTAGTGCTGTGGTCGATAATGAATTGAATGCTGTGAATGATGGTCACCTT
  GTAAGCAGTCCAGCCATTTGTAGTGGTAGCCTTAGTAACTTTTCAACCAGTGATAATGGGTCTTACAGCA
  GCAACGGTAGTGATTTTGGGTCATGTGCAAGTATCACAAGTGGAGGTTCATATACTAACAGTGTCATCAG
  TGACAGTAGTAGTTATACTTTTCCACCAAGTGATGATACTTTTTTGGGTGGAAACTTACCTTCTGACAGC
  ACCTCCAATAGAAGTGTGCCAAACAGGAATACTACTCCTTGTGAAATTTTTTCAAGAAGTACAAGTACAG
  ATCCTTTTGTCCAGGATGACTTGGAACATGGATTAGAGATTATGAAATTGCCAGTGAGCAGGAACACAAA
  AATTCCACTAAAACGTTACTCCTCCTTAGTCATTTTTCCTAGGAGTCCTTCAACTACCCGACCGACTTCT
  CCAACAAGTCTGTGTACTCTTCTGAGCAAAGGATCCTATCAAACTTCACACCAGTTTATTATTTCTCCTA
  GTGAAATTGCACATAATGAGGATGGCACTAGTGCTAAAGGATTTCTTTCAACAGCTGTCAATGGACTTCG
  GTTATCTAAAACAATTTGTACCCCCGGAGAAGTAAGAGACATACGGCCGCTTCACAGGAAGGGCTCGTTA
  CAGAAGAAAATTGTTCTTTCGAATAATACTCCCAGACAGACTGTCTGTGAAAAGTCATCTGAAGGATATT
  CTTGTGTTTCAGTGCATTTCACCCAACGAAAAGCAGCTACATTAGACTGTGAAACAACAAATGGTGATTG
  TAAACCAGAAATGTCAGAAATTAAGCTTAATTCTGATTCAGAGTATATTAAGCTCATGCATAGGACATCT
  GCATGTTTGCCATCCTCCCAAAATGTAGATTGTCAAATAAATATCAATGGAGAATTGGAAAGACCACATT
  CACAGATGAACAAAAACCATGGTATTTTACGAAGAAGTATTTCATTGGGAGGAGCTTATCCAAATATTTC
  CTGTCTATCCAGCCTTAAGCACAATTGTTCTAAAGGGGGACCATCTCAGTTACTCATAAAGTTTGCATCT
  GGAAATGAAGGTAAAGTGGATAATTTATCAAGAGACAGCAACAGAGATTGCACAAATGAACTGTCTAATT
  CTTGCAAGACAAGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATTACCGACAGAAAATCC
  AAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTCATCCAAGAAGTCACAAATCAAGAGTTAAA
  GGTTATCTGAGACTAAAAATGACTTATTTACCTAAAACCAGTGGCTCAGAAGATGATAATGCAGAACAGG
  CTGAGGAATTAGAGCCTGGCTGGGTTGTTTTGGACCAACCAGATGCTGCTTGCCATTTGCAGCAACAACA
  AGAACCTTCTCCTCTACCTCCAGGGTGGGAAGAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAAC
  CATGAATCTAGAAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTGAGAATGGCA
  ACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGGCAGATATCCGAGGAAACAGAAAGTGTTGA
  CAACCGAGAGTCTTCCGAGAACTGGGAAATTATAAGAGAAGATGAAGCCACCATGTATAGCAACCAGGCC
  TTCCCATCACCTCCACCGTCAAGTAACTTGGATGTTCCAACTCATCTTGCAGAAGAATTGAATGCCAGAC
  TCACCATTTTTGGAAATTCAGCCGTGAGCCAGCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAG
  CTTACAAGCCTATACTTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCTGGATTACCA
  CCAGGTTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATATTATGTAGATCACAATTCCAGAACGACTA
  CTTGGACAAAGCCCACTGTACAGGCCACAGTGGAGACCAGTCAGCTGACCTCAAGCCAGAGTTCTGCAGG
  CCCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGACCCAGCCATCTGAAATTGAGCAAGGA
  TTCCTTCCTAAAGGCTGGGAAGTCCGGCATGCACCAAATGGGAGGCCTTTCTTTATTGACCACAACACTA
  AAACCACCACCTGGGAAGATCCAAGATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATCACTTGATAC
  TTCCAATGATCTAGGGCCTTTACCTCCAGGATGGGAAGAGAGAACTCACACAGATGGAAGAATCTTCTAC
  ATAAATCACAATATAAAAAGAACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGGACCAG
  CAGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAAAGTTGAAGAAGCAGAATGA
  CATTCCAAACAAATTTGAAATGAAACTTCGCCGAGCAACTGTTCTTGAAGACTCTTACCGGAGAATTATG
  GGTGTCAAGAGAGCAGACTTCCTGAAGGCTCGACTGTGGATTGAGTTTGATGGTGAAAAGGGATTGGATT
  ATGGAGGAGTTGCCAGAGAATGGTTCTTCCTGATCTCAAAGGAAATGTTTAACCCTTATTATGGGTTGTT
  TGAATATTCTGCTACGGACAATTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCAC
  CTCTCTTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCATGGCAAACTGTTGGATGGTT
  TTTTCATCCGCCCATTTTACAAGATGATGCTTCACAAACCAATAACCCTTCATGATATGGAATCTGTGGA
  TAGTGAATATTACAATTCCCTAAGATGGATTCTTGAAAATGACCCAACAGAATTGGACCTCAGGTTTATC
  ATAGATGAAGAACTTTTTGGACAGACACATCAACATGAGCTGAAAAATGGTGGATCAGAAATAGTTGTCA
  CCAATAAGAACAAAAAGGAATATATTTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCA
  AATGGCTGCTTTTAAAGAGGGATTCTTTGAACTAATACCACAGGATCTCATCAAAATTTTTGATGAAAAT
  GAACTAGAGCTTCTTATGTGTGGACTGGGAGATGTTGATGTGAATGACTGGAGGGAACATACAAAGTATA
  AAAATGGCTACAGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTTAATGATGGATTCAGA
  AAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTCGGGTGCCTATGAATGGATTTGCTGAACTA
  TACGGTTCAAATGGACCACAGTCATTTACAGTTGAACAGTGGGGTACTCCTGAAAAGCTGCCAAGAGCTC
  ATACCTGTTTTAATCGCCTGGACTTGCCACCTTATGAATCATTTGAAGAATTATGGGATAAACTTCAGAT
  GGCAATTGAAAACACCCAGGGCTTTGATGGAGTTGATTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001284338
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001284338.1, NP_001271267.1
• RefSeq Size: 7240 bp
• RefSeq ORF: 3960 bp
• Locus ID: 4734
• UniProt ID: P46934
• Cytogenetics: 15q21.3
• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, Ubiquitin mediated proteolysis
• Gene Summary: This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]
  Transcript Variant: This variant (3) contains three alternate in-frame exons, compared to variant 2. The encoded isoform (3) is longer than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.