HA1 (ARHGAP45) (NM_001282335) Human Untagged Clone

CAT#: SC337781

HMHA1 (untagged) - Human histocompatibility (minor) HA-1 (HMHA1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARHGAP45
• Synonyms: HA-1; HLA-HA1; HMHA1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001282335, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGTATCTGCGGGACGGCGCACCCGGTGCTGGACGAGGGCCCCGTGCGCTGCCGGGCGGGCCCCCGAG
  ACCTCCTTGAGGCCCGCCGCCCGCGGGCCCACGAGTGCCTGGGTGAGGCTCTGCGTGTCATGCATCAGAT
  CATCTCCAAGTACCCGCTGCTGAACACCGTGGAGACGCTCACCGCAGCCGGCACCCTCATTGCCAAGGTC
  AAAGCCTTCCATTATGAGAGCAACAATGATCTGGAGAAACAGGAGTTCGAGAAGGCCCTGGAGACGATTG
  CTGTGGCCTTCAGTAGCACAGTGTCCGAGTTCCTCATGGGTGAAGTGGACAGCAGCACCCTCCTAGCAGT
  GCCTCCTGGGGACTCGAGCCAGTCCATGGAAAGCCTGTATGGACCGGGCAGTGAGGGCACGCCTCCCAGC
  CTGGAAGACTGTGACGCCGGCTGCCTGCCCGCCGAGGAGGTGGACGTGCTGCTACAGCGCTGTGAGGGGG
  GCGTGGATGCCGCACTGCTGTATGCCAAGAACATGGCCAAGTACATGAAGGACCTCATCAGCTACCTGGA
  GAAGCGGACGACGCTGGAGATGGAGTTTGCCAAGGGCCTGCAGAAGATCGCTCACAACTGCAGACAGAGC
  GTCATGCAGGAGCCCCACATGCCGCTCCTGTCCATCTACTCGCTGGCCCTGGAGCAGGACCTGGAGTTCG
  GCCACAGCATGGTGCAGGCGGTGGGCACCTTGCAGACCCAGACCTTCATGCAGCCCCTGACCCTGCGGCG
  GCTTGAACACGAGAAGCGCAGGAAGGAGATCAAGGAGGCCTGGCACCGTGCCCAGAGGAAGCTGCAAGAG
  GCGGAGTCCAACCTGCGCAAGGCCAAGCAGGGTTACGTGCAGCGCTGCGAGGACCACGACAAGGCTCGCT
  TCCTCGTGGCCAAGGCGGAGGAGGAGCAGGCTGGCAGCGCGCCGGGAGCAGGCAGCACGGCCACCAAGAC
  CCTGGACAAGCGGCGGCGGCTGGAGGAGGAGGCCAAGAACAAGGCGGAGGAAGCTATGGCCACCTACCGC
  ACCTGCGTGGCCGACGCGAAGACGCAGAAGCAGGAGCTGGAGGATACCAAGGTGACGGCGCTGCGGCAGA
  TCCAGGAGGTCATCCGGCAGAGCGACCAAACCATCAAGTCGGCCACGATCTCCTACTACCAGATGATGCA
  TATGCAGACGGCGCCGCTGCCCGTGCACTTCCAGATGCTGTGTGAGAGCAGCAAGCTGTATGACCCAGGC
  CAGCAGTACGCCTCCCACGTGCGCCAGCTGCAGCGGGACCAGGAGCCCGATGTGCACTACGACTTTGAGC
  CCCACGTCTCCGCCAACGCCTGGTCCCCCGTCATGCGTGCCCGGAAGAGCAGCTTCAACGTGAGTGATGT
  GGCGCGGCCGGAGGCTGCCGGGAGCCCCCCAGAAGAAGGCGGGTGCACTGAGGGCACACCTGCCAAGGAC
  CACAGGGCCGGGCGAGGACACCAGGTTCACAAGTCATGGCCGCTCTCGATCTCAGACTCGGACAGTGGGC
  TGGACCCCGGCCCTGGCGCAGGGGACTTTAAGAAGTTCGAGCGGACGTCATCCAGTGGTACCATGTCGTC
  CACGGAGGAGCTGGTGGACCCAGACGGTGGAGCCGGGGCTTCAGCCTTTGAGCAGGCTGACCTCAACGGC
  ATGACCCCCGAGCTGCCGGTGGCCGTGCCCAGTGGACCGTTCCGCCACGAGGGGCTGTCCAAGGCGGCCC
  GTACTCACCGGCTCCGGAAGCTCCGCACGCCCGCCAAGTGCCGCGAGTGCAACAGCTACGTCTACTTCCA
  GGGTGCTGAGTGTGAAGAGTGCTGCCTGGCCTGCCACAAGAAATGTCTGGAGACGCTGGCCATACAGTGC
  GGGCACAAGAAGCTGCAAGGCCGCCTGCAGCTGTTCGGCCAGGACTTCAGCCACGCGGCCCGCAGCGCCC
  CCGACGGCGTGCCCTTCATCGTCAAGAAGTGCGTCTGCGAGATCGAGCGGCGGGCGCTGCGCACCAAGGG
  CATCTACCGGGTCAATGGGGTAAAGACACGCGTGGAGAAGCTGTGCCAGGCCTTCGAGAACGGCAAGGAG
  CTGGTCGAGCTGTCGCAGGCCTCGCCCCACGACATCAGCAACGTCCTCAAGCTCTACCTGCGTCAGCTTC
  CCGAGCCGCTCATCTCCTTCCGCCTCTACCACGAGCTCGTAGGGCTGGCCAAGGACAGCCTGAAGGCAGA
  GGCCGAGGCCAAGGCGGCGTCCCGGGGCCGGCAGGACGGCTCGGAGAGCGAGGCAGTGGCGGTGGCCCTG
  GCAGGTCGGCTGCGGGAGCTCCTGCGGGACCTGCCGCCTGAGAACCGGGCCTCGCTGCAGTACCTGCTGC
  GTCACCTACGCAGGATCGTGGAGGTGGAGCAGGACAACAAGATGACCCCCGGGAACCTGGGCATCGTGTT
  CGGGCCCACGCTGCTTCGGCCACGGCCCACCGAGGCCACCGTGTCCCTCTCCTCCCTGGTGGATTATCCC
  CATCAGGCCCGCGTCATCGAGACTCTCATCGTCCACTACGGCCTGGTCTTCGAGGAGGAGCCGGAGGAGA
  CCCCCGGGGGCCAGGACGAGTCATCCAACCAGCGAGCTGAGGTAGTCGTCCAGGTGCCGTACCTGGAGGC
  GGGCGAGGCGGTGGTCTACCCGCTGCAGGAGGCGGCGGCGGACGGGTGCAGAGAATCCCGAGTTGTGTCC
  AACGATTCGGACTCGGACCTAGAGGAGGCCTCCGAGCTGCTGTCCTCATCGGAGGCCAGTGCCCTGGGCC
  ACCTCAGCTTCCTGGAGCAGCAGCAGAGCGAGGCCAGCCTAGAGGTGGCTTCTGGCAGCCACAGCGGCAG
  TGAGGAGCAGCTGGAGGCCACAGCCCGGGAGGACGGGGACGGGGACGAGGACGGCCCGGCCCAGCAGCTC
  TCAGGATTCAACACCAACCAGTCCAACAACGTGCTGCAGGCCCCACTGCCCCCCATGAGGCTCCGTGGCG
  GGCGGATGACACTGGGCTCCTGCAGGGAAAGGCAGCCGGAATTCGTGTGA
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001282335
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282335.1, NP_001269264.1
• RefSeq Size: 4965 bp
• RefSeq ORF: 3060 bp
• Locus ID: 23526
• UniProt ID: Q92619
• Cytogenetics: 19p13.3
• Gene Summary: Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) uses an alternate internal promoter and thus differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at an alternate AUG, and the resulting isoform (4) has a shorter and distinct N-terminus compared to isoform 1.