PHF17 (JADE1) (NM_001287437) Human Untagged Clone

CAT#: SC337538

JADE1 (untagged) - Human jade family PHD finger 1 (JADE1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: JADE1
• Synonyms: PHF17
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001287437, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAACGAGGTCGCCTTCCCAGCAGCAGTGAGGATTCTGACGACAATGGCAGCCTGTCAACTACTTGGT
  CCCAGAATTCCCGATCCCAGCATAGGAGAAGCTCCTGCTCCAGACATGAAGATCGAAAGCCTTCAGAGGT
  GTTTAGGACAGACCTGATCACTGCCATGAAGTTGCATGACTCCTACCAGCTGAATCCGGATGAGTACTAT
  GTGTTGGCAGATCCCTGGAGACAGGAATGGGAGAAAGGGGTCCAGGTGCCTGTGAGCCCGGGGACCATCC
  CTCAGCCTGTGGCCAGGCCCAAGAAGTACATCGTGTCATCAGGCTCTGAGCCTCCCGAGTTGGGCTATGT
  GGACATCCGGACGCTGGCTGACAGCGTGTGTCGCTATGACCTCAATGACATGGATGCTGCATGGCTGGAA
  CTGACCAATGAAGAATTTAAGGAGATGGGAATGCCTGAACTAGATGAATACACCATGGAGAGGGTCCTAG
  AGGAATTTGAGCAGCGATGCTACGACAATATGAATCATGCCATAGAGACTGAGGAAGGCCTGGGGATCGA
  ATATGATGAAGATGTTGTCTGTGATGTCTGCCAGTCTCCTGATGGTGAGGACGGCAATGAGATGGTGTTC
  TGTGACAAATGCAACATCTGTGTGCACCAGGCCTGTTATGGAATCCTCAAGGTACCAGAGGGCAGCTGGC
  TGTGCCGGACATGTGCCCTGGGGGTTCAGCCAAAATGTCTGCTGTGTCCGAAGAAGGGTGGAGCTATGAA
  GCCCACCCGTAGCGGAACCAAGTGGGTCCACGTTAGCTGTGCTCTGTGGATCCCTGAGGTGAGCATTGGC
  AGCCCAGAGAAGATGGAGCCCATCACCAAGGTGTCACACATTCCCAGCAGCCGGTGGGCGCTAGTGTGCA
  GCCTCTGCAATGAGAAGTTTGGGGCCTCTATACAGTGCTCTGTGAAGAACTGCCGCACAGCCTTCCATGT
  GACCTGTGCTTTTGACCGGGGCCTGGAGATGAAGACCATCTTAGCAGAGAATGATGAAGTCAAGTTCAAG
  TCCTATTGCCCAAAGCACAGCTCACATAGGAAACCCGAGGAGAGTCTTGGCAAGGGGGCTGCACAGGAGA
  ATGGGGCCCCTGAGTGTTCCCCCCGGAATCCGCTGGAGCCCTTTGCCAGCCTTGAGCAGAACCGGGAGGA
  GGCCCACCGGGTGAGTGTCCGTAAGCAGAAGCTGCAGCAGTTGGAGGATGAGTTCTACACCTTCGTCAAC
  CTGCTGGATGTTGCCAGGGCTCTGCGGCTGCCTGAGGAAGTAGTGGATTTCCTGTACCAGTACTGGAAGT
  TGAAGAGGAAGGTCAACTTCAACAAGCCCCTGATCACCCCAAAGAAAGATGAAGAGGACAATCTAGCCAA
  GCGGGAGCAGGATGTCTTATTTAGGAGGCTGCAGCTGTTCACGCACCTGCGGCAGGACCTGGAGAGGGTT
  CGGAACCTCACTTACATGGTGACCCGCAGGGAAAAGATTAAACGGTCTGTGTGCAAAGTCCAGGAACAGA
  TATTCAATCTTTACACTAAGCTTTTGGAGCAAGAAAGAGTTTCAGGTGTGCCTTCTTCCTGCTCCTCCTC
  CTCACTGGAAAACATGCTTTTGTTTAACAGTCCTTCTGTTGGCCCTGATGCTCCCAAGATAGAGGACTTG
  AAGTGGCATTCTGCATTCTTCAGAAAACAAATGGGTACTTCCTTGGTTCATTCGCTGAAAAAGCCCCATA
  AGCGAGATCCTTTGCAGAATAGCCCTGGAAGTGAAGGCAAAACCCTGCTGAAGCAGCCAGACCTGTGTGG
  TAGAAGGGAGGGGATGGTGGTCCCAGAGAGCTTTTTGGGTTTAGAAAAGACCTTTGCAGAAGCACGTCTC
  ATATCAGCACAACAGAAAAATGGTGTGGTGATGCCAGACCATGGGAAAAGAAGAGACAATCGTTTTCATT
  GTGATCTCATTAAAGGAGACTTAAAGGACAAATCTTTTAAACAGAGTCACAAGCCTCTCAGGTCCACAGA
  TGTGTCCCAGAGGCATCTGGACAACACAAGAGCTGCCACCTCCCCTGGAGTGGGGCAGTCAGCACCTGGC
  ACAAGGAAGGAGATAGTGCCCAAGTGCAATGGCTCCCTAATCAAAGTAAACTATAATCAGACTGCAGTCA
  AAGTGCCTACAACACCTGCCAGCCCAGTGAAAAACTGGGGAGGATTCCGGATTCCAAAGAAGGGGGAACG
  GCAGCAGCAGGGAGAGGCCCACGATGGGGCCTGCCACCAGCACTCAGACTACCCATATTTGGGCTTAGGC
  CGAGTTCCAGCCAAGGAAAGGGCAAAAAGCAAATTAAAATCCGACAATGAGAATGACGGGTATGTCCCCG
  ATGTGGAAATGAGTGACTCAGAGAGTGAGGCATCAGAAAAGAAATGTATACACACCAGCAGCACTATCAG
  CAGAAGGACAGACATTATCAGGAGAAGCATCTTGGCTTCTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287437
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287437.1, NP_001274366.1
• RefSeq Size: 5736 bp
• RefSeq ORF: 2493 bp
• Locus ID: 79960
• UniProt ID: Q6IE81
• Cytogenetics: 4q28.2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Transcriptional coactivator, it may also promote acetylation of nucleosomal histone H4 by KAT5. Promotes apoptosis. May act as a renal tumor suppressor. Negatively regulates canonical Wnt signaling; at least in part, cooperates with NPHP4 in this function.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.