INPP5F (NM_001243194) Human Untagged Clone

CAT#: SC336600

INPP5F (untagged) - Human inositol polyphosphate-5-phosphatase F (INPP5F), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: INPP5F
• Synonyms: hSAC2; MSTP007; MSTPO47; SAC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336600 representing NM_001243194.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGTCATGACGTAATTTTTATGGCTTGGCTCAAGCAACAATTTTCAGAGTGCACCCTCATTGATGCT
  ACTCACAGAGACGTGGATGTGCTGTTACTGCTTTCTAACTCTGCCTACTACGTGGCCTATTATGATGAT
  GAAGTTGATAAAGTAAACCAGTATCAACGACTAAGTCTAGAAAACCTGGAAAAAATTGAAATAGGCCCT
  GAACCCACTCTTTTTGGTAAGCCAAAGTTCTCCTGCATGCGACTGCACTACAGATACAAAGAAGCGAGT
  GGCTATTTCCACACATTGCGAGCTGTAATGCGTAATCCTGAAGAGGATGGAAAAGATACCCTTCAGTGC
  ATTGCAGAGATGCTGCAGATCACCAAGCAAGCCATGGGATCGGATTTACCCATAATTGAGAAGAAACTT
  GAGAGGAAGAGCAGTAAACCTCACGAAGACATCATTGGTATCAGGTCTCAAAACCAAGGTTCTTTGGCC
  CAGGGAAAGAATTTTTTAATGAGCAAATTTTCATCTCTAAATCAAAAAGTGAAGCAGACCAAATCCAAT
  GTAAATATTGGCAACCTCCGAAAGCTAGGAAACTTTACCAAACCTGAAATGAAAGTTAACTTTCTAAAA
  CCAAACTTAAAAGTAAATCTTTGGAAATCAGATAGTAGTCTTGAAACTATGGAAAACACAGGAGTGATG
  GATAAGGTTCAGGCAGAGTCTGATGGGGACATGTCTTCAGATAATGACTCATACCACTCTGATGAATTC
  CTTACAAATTCTAAGTCTGATGAAGACAGGCAGCTAGCTAACTCATTAGAGAGTGTAGGGCCAATAGAT
  TACGTTCTTCCTAGTTGTGGTATTATTGCCTCAGCGCCTCGATTGGGCAGTCGGTCCCAGTCTCTTAGC
  AGCACAGATAGTAGCGTTCATGCTCCTTCAGAGATTACTGTTGCTCATGGGAGTGGGCTTGGAAAAGGC
  CAGGAGTCTCCTTTGAAGAAAAGTCCTTCTGCTGGCGACGTACACATATTGACTGGCTTTGCCAAGCCT
  ATGGATATTTACTGCCACAGATTTGTGCAAGATGCACAGAACAAAGTGACCCACCTATCAGAGACCAGA
  TCTGTGTCTCAGCAGGCTAGTCAGGAAAGAAATCAAATGACCAATCAAGTTTCAAATGAAACCCAATCA
  GAATCAACAGAACAGACACCTTCTCGGCCATCGCAATTAGATGTCTCTCTTTCTGCAACAGGCCCACAG
  TTTTTGTCAGTTGAGCCAGCGCATTCAGTTGCATCTCAAAAAACCCCCACCTCCGCTTCCAGCATGCTT
  GAACTTGAGACAGGGCTTCATGTAACTCCTTCTCCTTCAGAGAGCAGTAGCAGCAGAGCAGTCTCTCCC
  TTTGCCAAGATTCGAAGTTCCATGGTCCAGGTTGCTAGTATTACCCAAGCTGGATTAACCCATGGGATA
  AACTTTGCAGTGTCAAAAGTTCAGAAGAGTCCTCCAGAACCTGAAATCATTAATCAAGTCCAGCAAAAT
  GAACTTAAAAAGATGTTTATACAATGCCAGACACGGATAATTCAGATTTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001243194
• Insert Size: 1569 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001243194.1
• RefSeq Size: 3167 bp
• RefSeq ORF: 1569 bp
• Locus ID: 22876
• UniProt ID: Q9Y2H2
• Cytogenetics: 10q26.11
• Protein Families: Druggable Genome
• MW: 57.8 kDa
• Gene Summary: The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase and contains a Sac domain. The activity of this protein is specific for phosphatidylinositol 4,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region, and uses an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.