ELF2 (NM_001276459) Human Untagged Clone

CAT#: SC336436

ELF2 (untagged) - Human E74-like factor 2 (ets domain transcription factor) (ELF2), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ELF2
• Synonyms: b; EU32; NERF; NERF-1A; NERF-1a; NERF-1B; NERF-2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336436 representing NM_001276459.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGACGTCTCTGCATGAGGGACCCACGAACCAGCTGGATCTGCTCATCCGGGCCGTGGAAGCATCA
  GTTCACAGCAGTAATGCACACTGTACAGATAAGACAATTGAAGCTGCTGAAGCCCTGCTTCATATGGAA
  TCTCCTACCTGCTTGAGGGATTCAAGAAGTCCTGAATTCATCCATGCTGCTATGAGGCCAGATGTCATT
  ACAGAAACTGTAGTGGAGGTGTCAACTGAAGAGTCTGAACCCATGGATACCTCTCCTATTCCAACATCA
  CCAGATAGCCATGAACCAATGAAAAAGAAAAAAGGAAACACAACCTATTTGTGGGAGTTTCTTTTAGAT
  CTACTTCAAGATAAAAATACTTGTCCCAGGTATATTAAATGGACTCAGAGAGAAAAAGGCATATTCAAG
  CTGGTGGATTCAAAGGCTGTCTCTAAGCTTTGGGGAAAGCATAAGAACAAACCAGACATGAACTATGAA
  ACCATGGGACGAGCTTTGAGATACTACTACCAAAGGGGAATTCTTGCAAAGGTTGAAGGACAGAGGCTT
  GTATATCAGTTCAAGGATATGCCGAAAAACATAGTGGTCATAGATGATGACAAAAGTGAAACCTGTAAT
  GAAGATTTAGCAGGAACTACTGATGAAAAATCATTAGAACGAGTGTCACTGTCTGCAGAAAGTCTCCTG
  AAAGCAGCATCCTCTGTTCGCAGTGGAAAAAATTCATCCCCTATAAACTGCTCCAGAGCAGAGAAGGGT
  GTAGCTAGAGTTGTGAATATCACTTCCCCTGGGCACGATGCTTCATCCAGGTCTCCTACTACCACTGCA
  TCTGTGTCAGCAACAGCAGCTCCAAGGACAGTTCGTGTGGCAATGCAGGTACCTGTTGTAATGACATCA
  TTGGGTCAGAAAATTTCAACTGTGGCAGTTCAGTCAGTTAATGCAGGTGCACCATTAATAACCAGCACT
  AGTCCAACAACAGCGACCTCTCCAAAGGTAGTCATTCAGACAATCCCTACTGTGATGCCAGCTTCTACT
  GAAAATGGAGACAAAATCACCATGCAGCCTGCCAAAATTATTACCATCCCAGCTACACAGCTTGCACAG
  TGTCAACTGCAGACAAAGTCAAATCTGACTGGATCAGGAAGCATTAACATTGTTGGAACCCCATTGGCT
  GTGAGAGCACTTACCCCTGTTTCAATAGCCCATGGTACACCTGTAATGAGACTATCAATGCCTACTCAG
  CAGGCATCTGGCCAGACTCCTCCTCGAGTTATCAGTGCAGTCATAAAGGGGCCAGAGGTTAAATCGGAA
  GCAGTGGCAAAAAAGCAAGAACATGATGTGAAAACTTTGCAGCTAGTAGAAGAAAAACCAGCAGATGGA
  AATAAGACAGTGACCCACGTAGTGGTTGTCAGTGCGCCTTCAGCTATTGCCCTTCCTGTAACTATGAAA
  ACAGAAGGACTAGTGACATGTGAGAAATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001276459
• Insert Size: 1479 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276459.1
• RefSeq Size: 2969 bp
• RefSeq ORF: 1479 bp
• Locus ID: 1998
• UniProt ID: Q15723
• Cytogenetics: 4q31.1
• Protein Families: Druggable Genome, Transcription Factors
• MW: 53 kDa
• Gene Summary: Isoform 1 transcriptionally activates the LYN and BLK promoters and acts synergistically with RUNX1 to transactivate the BLK promoter.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (5) has an alternate exon in place of the first three exons and lacks an alternate in-frame exon compared to variant 1. Translation starts in the alternate exon, so the resulting isoform (5) has a shorter and distinct N-terminus and lacks an alternate internal segment compared to isoform 1.