ZWILCH (NM_001287823) Human Untagged Clone

CAT#: SC336348

ZWILCH (untagged) - Human zwilch kinetochore protein (ZWILCH), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZWILCH
• Synonyms: hZwilch; KNTC1AP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC336348 representing NM_001287823.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTA
  CCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCC
  ATGGCTCACAATCCTAATATGACCCATTTGAAGATTAATCTGCCAGTTACTGCCCTTCCTCCCCTTTGG
  GTAAGATGTGACAGTTCAGATCCTGAAGGTACTTGTTGGCTAGGAGCTGAGCTTATCACAACAAACAAC
  AGCATTACAGGAATTGTCTTATATGTGGTCAGTTGTAAAGCTGATAAAAATTATTCTGTAAATCTTGAA
  AACCTAAAAAATTTACACAAGAAAAGACATCACTTGTCTACTGTAACATCCAAAGGCTTTGCCCAGTAT
  GAGCTCTTTAAGTCCTCTGCCTTGGATGATACAATCACAGCATCACAAACTGCGATCGCTTTGGATATT
  TCCTGGAGTCCTGTGGATGAGATTCTTCAAATCCCTCCACTCTCTTCAACTGCAACTCTGAATATTAAA
  GTGGAATCAGGAGAGCCCAGAGGTCCTTTGAATCATCTCTACAGAGAACTGAAATTTCTTCTTGTTTTG
  GCTGATGGTTTGAGGACTGGTGTCACTGAATGGCTCGAGCCCCTGGAAGCAAAATCTGCTGTTGAACTT
  GTTCAGGAATTTCTGAATGACTTAAATAAGCTGGATGGATTTGGTGATTCTACAAAAAAAGACACTGAG
  GTTGAGACCTTGAAGCATGACACTGCTGCAGTCGATCGTTCCGTCAAGCGTCTTTTCAAAGTTCGGAGT
  GATCTTGATTTTGCTGAGCAACTGTGGTGCAAAATGAGCAGTAGTGTGATTTCATACCAAGACTTGGTG
  AAGTGTTTCACATTGATCATCCAGAGTCTACAACGTGGTGATATACAGCCATGGCTCCATAGTGGAAGT
  AACAGTTTACTAAGTAAGCTCATTCATCAGTCTTATCATGGAACCATGGACACAGTTTCTCTCAGTGGG
  ACTATTCCAGTTCAAATGCTTTTGGAAATTGGTTTGGACAAACTAAAGAAAGATTATATCAGTTTTTTC
  ATAGGTCAGGAACTTGCATCTTTGAATCATTTGGAATACTTCATTGCTCCATCAGTAGATATACAAGAA
  CAGGTTTATCGTGTCCAAAAACTCCACCATATTCTAGAAATATTAGTCAGTTGCATGCCTTTCATTAAA
  TCTCAACATGAACTCCTCTTTTCTTTAACACAGATCTGCATAAAGTATTACAAACAAAATCCTCTTGAT
  GAGCAACACATTTTTCAGCTGCCAGTCAGACCAACTGCTGTAAAGAACTTATATCAAAGTGAGAAGCCA
  CAGAAATGGAGAGTGGAAATATATAGTGGTCAAAAGAAGATTAAGACAGTTTGGCAACTGAGTGACAGC
  TCACCCATAGACCATCTGAATTTTCACAAACCTGATTTTTCGGAATTAACACTAAACGGTAGCCTGGAA
  GAAAGGATATTCTTTACTAACATGGTTACCTGCAGCCAGGTGCATTTCAAGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: AscI-MluI
• ACCN: NM_001287823
• Insert Size: 1434 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_001287823.1
• RefSeq Size: 3152 bp
• RefSeq ORF: 1434 bp
• Locus ID: 55055
• UniProt ID: Q9H900
• Cytogenetics: 15q22.31
• MW: 54.2 kDa
• Gene Summary: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex (PubMed:15824131).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (4) lacks an internal exon, compared to variant 1. This difference results in predicted translation initiation at a downstream in-frame start site through ribosomal re-initiation or leaky scanning. The encoded isoform (2) is shorter than isoform 1. Variants 2, 3, and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.