TBC1D2 (NM_001267572) Human Untagged Clone

CAT#: SC332856

TBC1D2 (untagged) - Homo sapiens TBC1 domain family, member 2 (TBC1D2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBC1D2
• Synonyms: PARIS-1; PARIS1; TBC1D2A
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332856 representing NM_001267572.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGAAGCTTACCGGACCCAGAACTGCTTCCTCAACTCCGAGATCCACCAGGTCACAAAGATCTGGAGA
  AAGGTGGCTGAGAAGGAGAAGGCCCTTCTGACGAAGTGCGCCTACCTCCAAGCCAGAAACTGCCAGGTG
  GAAAGCAAGTACCTGGCCGGTCTGAGAAGGCTGCAGGAGGCCCTGGGGGACGAAGCCAGCGAGTGCTCA
  GAGCTGCTGAGGCAGCTTGTCCAGGAGGCACTGCAGTGGGAAGCTGGGGAGGCCTCATCTGACAGCATC
  GAGCTGAGCCCCATCAGTAAGTATGATGAGTACGGCTTCCTGACGGTGCCCGACTATGAGGTGGAAGAC
  CTGAAGCTGCTGGCCAAGATCCAGGCATTGGAGTCACGATCCCACCACCTGCTGGGCCTCGAGGCTGTG
  GATCGGCCGCTGAGGGAGCGCTGGGCTGCCCTGGGCGATCTTGTGCCCTCAGCCGAGCTCAAGCAGCTA
  CTGCGGGCAGGAGTACCCCGTGAACACCGGCCTCGTGTCTGGAGGTGGCTGGTCCACCTCCGTGTCCAG
  CACCTGCACACTCCAGGCTGCTACCAGGAACTGCTGAGCCGGGGCCAGGCCCGCGAGCACCCTGCTGCC
  CGCCAGATTGAGCTGGACCTGAACCGGACCTTCCCCAACAACAAACACTTCACCTGCCCCACCTCCAGC
  TTCCCCGACAAGCTCCGCCGGGTGCTGCTGGCCTTCTCCTGGCAGAACCCCACCATCGGCTACTGCCAG
  GGCCTGAACAGGCTGGCGGCCATTGCCCTGCTGGTCCTAGAGGAGGAGGAGAGCGCCTTCTGGTGCCTG
  GTGGCCATTGTGGAGACCATCATGCCCGCTGATTACTACTGCAACACGCTGACGGCATCCCAGGTGGAC
  CAGCGGGTGCTCCAGGACCTGCTCTCGGAGAAGCTGCCCAGGCTGATGGCCCATCTGGGGCAGCACCAC
  GTGGATCTCTCCCTCGTCACCTTCAACTGGTTCCTCGTGGTCTTTGCGGACAGTCTCATTAGCAACATC
  CTCCTTCGGGTCTGGGATGCCTTCCTGTACGAGGGGACGAAGGTGGTGTTTCGCTATGCCTTGGCCATT
  TTCAAGTACAACGAGAAGGAGATCTTGAGGCTACAGAATGGCCTGGAAATCTACCAGTACCTGCGCTTC
  TTCACCAAGACCATCTCCAACAGCCGGAAGCTGATGAACATCGCCTTCAATGACATGAACCCCTTCCGC
  ATGAAACAGCTGCGGCAGCTGCGCATGGTCCACCGGGAGCGGCTGGAGGCTGAGCTGCGGGAGCTGGAG
  CAGCTTAAGGCAGAGTACCTGGAGAGGCGGGCATCCCGGCGCAGAGCTGTGTCCGAGGGCTGTGCCAGC
  GAGGACGAGGTGGAGGGGGAAGCCTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001267572
• Insert Size: 1407 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267572.1
• RefSeq Size: 2142 bp
• RefSeq ORF: 1407 bp
• Locus ID: 55357
• UniProt ID: Q9BYX2
• Cytogenetics: 9q22.33
• MW: 54.4 kDa
• Gene Summary: Acts as GTPase-activating protein for RAB7A. Signal effector acting as a linker between RAC1 and RAB7A, leading to RAB7A inactivation and subsequent inhibition of cadherin degradation and reduced cell-cell adhesion.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and represents the use of an alternate promoter, compared to variant 1, which results in the use of an in-frame downstream start codon and a protein (isoform 3) with a shorter N-terminus, compared to isoform 1.