CNOT10 (NM_001256741) Human Untagged Clone

CAT#: SC332459

CNOT10 (untagged) - Homo sapiens CCR4-NOT transcription complex, subunit 10 (CNOT10), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CNOT10
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC332459 representing NM_001256741.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGCTGCAGACAAGCCTGCAGATCAGGGAGCAGAGAAACATGAAGGCACAGGTCAGTCCTCTGGGATC
  ACTGATCAAGAGAAGGAGTTATCCACCAATGCTTTCCAAGCTTTCACATCTGGAAATTATGATGCCTGT
  CTACAACACCTTGCCTGTCTACAAGATATAAACAAAGATGATTATAAAATAATTTTGAATACAGCAGTA
  GCTGAGTTTTTTAAAAGTAACCAAACAACAACAGATAATTTGAGACAAACACTTAACCAGCTGAAGAAT
  CAGGTCCACTCAGCTGTTGAAGAAATGGATGGATTAGATGATGTTGAAAACAGCATGTTGTACTATAAT
  CAAGCAGTCATTCTTTATCATCTGCGGCAGTATACAGAAGCCATATCAGTTGGTGAAAAACTTTATCAG
  TTCATAGAGCCTTTTGAAGAAAAATTTGCCCAAGCAGTGTGTTTTTTGCTTGTAGACCTGTATATATTA
  ACCTACCAAGCTGAGAAAGCTTTGCATCTTCTTGCTGTCCTAGAAAAAATGATTTCACAGGGTAACAAT
  AACAAAAATGGAAAGAATGAGACTGGTAATAACAACAACAAAGATGGATCTAATCATAAAGCTGAAAGT
  GGAGCTCTAATAGAAGCTGCAAAATCAAAGATACATCAGTACAAAGTACGAGCTTATATCCAAATGAAG
  TCTCTGAAAGCATGTAAAAGGGAAATCAAGTCAGTCATGAATACAGCTGGAAATTCCGCACCCTCTCTC
  TTTCTTAAAAGCAATTTTGAGTACTTAAGAGGTAATTATCGAAAAGCCGTGAAGCTATTAAATAGTTCA
  AACATTGCTGAGCATCCAGGATTCATGAAAACAGGTGAATGCTTGAGATGCATGTTCTGGAATAACCTT
  GGTTGCATCCATTTTGCCATGAGCAAGCACAATTTGGGAATATTCTACTTTAAAAAGGCTCTGCAAGAG
  AATGACAATGTCTGTGCACAGCTCAGTGCAGGTAGCACTGATCCAGGTAAAAAATTTTCAGGAAGACCC
  ATGTGTACGTTACTAACCAATAAGAGATATGAGTTGCTGTATAACTGTGGAATTCAGCTTCTTCACATT
  GGAAGGCCTCTTGCTGCCTTCGAATGTCTGATTGAAGCTGTTCAGGTTTATCATGCAAATCCTCGCCTC
  TGGCTACGGCTGGCTGAATGCTGCATTGCTGCCAATAAGGGGACTTCTGAACAAGAAACTAAAGGCCTT
  CCCAGCAAAAAAGGAATTGTACAGTCTATTGTTGGTCAAGGCTATCATCGTAAAATAGTTTTGGCATCA
  CAGTCTATACAGAATACTGTTTATAATGATGGGCAGTCTTCGGCCATTCCTGTAGCCAGTATGGAGTTT
  GCAGCCATATGTCTCAGAAATGCCTTGTTGCTGCTACCTGAAGAACAGCAAGATCCAAAGCAGGAAAAT
  GGGGCTAAAAATAGTAATCAATTAGGTGGGAACACAGAGAGCAGCGAAAGCAGTGAAACTTGCAGGTGC
  TCCATACTTGCTTGCAGTGCCTACGTGGCTCTGGCTTTGGGTGATAACCTCATGGCTTTGAATCATGCA
  GATAAACTTCTTCAGCAGCCCAAGCTGTCAGGATCTCTTAAGTTTTTGGGACATTTATATGCTGCAGAA
  GCCCTCATCTCTCTCGACAGAATATCTGATGCCATTACTCACTTGAACCCGGAGAATGTCACTGATGTC
  TCCTTAGGGATCTCTTCAAATGAGCAGGACCAAGGATCAGACAAAGGTGAAAATGAAGCAATGGAATCC
  TCTGGTAAGCGGGCCCCTCAGTGCTACCCCAGTTCCGTCAACTCTGCCAGGACTGTGATGCTGTTCAAC
  CTTGGCAGCGCTTACTGCCTGAGGAGCGAATATGACAAAGCCCGAAAGTGTCTCCACCAGGCGGCTTCA
  ATGATCCATCCTAAAGAGGTGCCCCCTGAGGCCATCTTGCTGGCAGTCTACCTTGAACTGCAGAATGGT
  AATACTCAGCTGGCCTTACAGATCATCAAAAGGAATCAGCTGCTCCCTGCAGTGAAAACACACTCTGAA
  GTGAGAAAGAAGCCAGTGTTTCAGCCTGTCCACCCGATCCAGCCCATCCAAATGCCGGCTTTCACCACT
  GTGCAGAGAAAGTGA
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001256741
• Insert Size: 2154 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001256741.1
• RefSeq Size: 2748 bp
• RefSeq ORF: 2154 bp
• Locus ID: 25904
• UniProt ID: Q9H9A5
• Cytogenetics: 3p22.3
• Protein Pathways: RNA degradation
• MW: 79.4 kDa
• Gene Summary: Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.