ASPM (NM_001206846) Human Untagged Clone

CAT#: SC331694

ASPM (untagged) - Homo sapiens asp (abnormal spindle) homolog, microcephaly associated (Drosophila) (ASPM), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASPM
• Synonyms: ASP; Calmbp1; MCPH5
• Vector: pCMV6-Entry
• Sequence Data:
  Fully Sequenced ORF

  >SC331694 representing NM_001206846.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  ATGGCGAACCGGCGAGTGGGGCGAGGCTGCTGGGAAGTGAGCCCGACCGAGCGGAGGCCGCCCGCGGGG
  CTGCGGGGCCCCGCGGCCGAGGAGGAGGCGTCTTCCCCGCCGGTCCTGTCTCTCAGCCACTTCTGCAGG
  TCTCCTTTCCTTTGCTTCGGGGACGTTCTCCTGGGAGCCTCACGGACGCTGTCTCTGGCCCTAGACAAC
  CCTAACGAGGAGGTGGCAGAAGTGAAGATCTCCCACTTCCCGGCCGCGGACCTGGGCTTCAGTGTGTCG
  CAGCGCTGTTTCGTGTTGCAGCCTAAAGAGAAAATTGTTATTTCTGTTAACTGGACACCACTCAAAGAA
  GGCCGAGTAAGAGAGATTATGACATTTCTTGTAAATGATGTTCTGAAACACCAAGCTATATTACTAGGA
  AATGCAGAAGAGCAGAAAAAGAAAAAGAGGAGTCTTTGGGATACCATTAAAAAGAAGAAAATTTCAGCC
  TCTACAAGTCACAACAGAAGGGTTTCAAATATTCAGAATGTTAATAAAACATTTAGTGTTTCCCAAAAA
  GTTGACAGAGTTAGGAGCCCACTACAAGCTTGTGAAAACTTGGCTATGAATGAAGGCGGTCCCCCAACA
  GAAAACAATTCTTTAATACTTGAAGAAAATAAAATACCCATATCACCTATTAGCCCTGCTTTCAATGAA
  TGCCATGGTGCAACTTGCTTGCCACTCTCTGTACGTCGATCTACTACCTACTCATCTCTTCATGCATCA
  GAAAATAGGGAACTATTAAATGTACACAGTGCCAACGTTTCAAAAGTTTCTTTTAATGAGAAAGCTGTA
  ACTGAAACTTCCTTTAATTCCGTAAATGTTAATGGCCAAAGAGGAGAGAATAGTAAACTTAGTCTTACC
  CCCAACTGTTCTTCAACTTTGAACATTACACAAAGCCAAATACATTTTCTAAGTCCAGATTCTTTTGTA
  AATAATAGTCATGGAGCTAATAATGAACTAGAATTAGTAACATGTCTTTCATCAGATATGTTTATGAAA
  GATAATTCACAGCCTGTGCATTTGGAATCAACAATTGCACATGAAATTTATCAGAAAATTTTAAGTCCA
  GATTCTTTCATAAAAGATAATTATGGACTAAATCAGGATCTAGAATCAGAGTCAGTTAATCCTATTTTA
  TCCCCTAATCAATTTTTAAAAGATAACATGGCATATATGTGTACATCTCAGCAAACATGTAAAGTACCA
  TTATCAAATGAAAATTCTCAAGTCCCACAGTCTCCTGAAGATTGGAGAAAAAGTGAAGTTTCGCCACGT
  ATTCCTGAATGTCAGGGTTCAAAATCTCCCAAAGCTATTTTTGAAGAACTAGTAGAAATGAAGTCAAAT
  TACTACAGTTTTATAAAACAAAATAATCCTAAATTTTCTGCAGTTCAGGATATTTCTAGTCATAGCCAC
  AATAAACAACCTAAGAGACGTCCAATACTTTCTGCCACTGTTACTAAAAGGAAGGCCACCTGTACCAGA
  GAAAACCAAACTGAGATTAATAAACCAAAAGCAAAAAGATGTCTCAACAGTGCAGTGGGTGAACATGAA
  AAAGTAATAAATAATCAAAAGGAAAAAGAAGATTTTCATTCTTATCTTCCAATTATAGATCCAATATTA
  AGTAAATCTAAGAGTTATAAAAACGAGGTAACACCCTCTTCGACAACAGCTTCAGTTGCTCGGAAAAGA
  AAGAGCGATGGAAGCATGGAAGATGCAAATGTGAGAGTTGCAATTACAGAACATACAGAAGTGCGAGAA
  ATCAAAAGAATCCATTTTTCTCCCTCAGAGCCTAAAACATCAGCTGTTAAGAAAACAAAAAATGTGACA
  ACACCCATCTCAAAACGTATTAGCAACAGAGAGAAATTAAACCTGAAGAAGAAAACTGATTTATCAATA
  TTCAGAACTCCAATTTCTAAAACAAACAAAAGGACAAAACCCATTATCGCTGTGGCACAGTCCAGTTTG
  ACCTTCATAAAACCATTAAAAACAGATATTCCCAGACACCCGATGCCATTTGCTGCAAAAAACATGTTT
  TATGATGAACGCTGGAAGGAAAAGCAGGAACAGGGCTTCACTTGGTGGTTAAATTTTATATTAACCCCT
  GATGACTTCACTGTAAAAACAAATATTTCTGAAGTAAATGCTGCTACTCTTCTTTTGGGAATAGAGAAT
  CAACATAAAATAAGTGTTCCTAGAGCACCTACAAAAGAGGAAATGTCTCTCAGAGCTTATACTGCTCGG
  TGTAGGTTAAACAGACTACGTCGTGCAGCATGCCGTTTGTTTACTTCTGAAAAAATGGTTAAAGCTATT
  AAAAAGCTTGAAATTGAAATTGAAGCTAGGCGGTTAATTGTTCGAAAAGATAGACACCTATGGAAAGAT
  GTGGGAGAACGTCAGAAAGTCCTGAATTGGCTGTTGTCCTACAATCCTTTGTGGCTTCGAATTGGTCTA
  GAGACAACTTATGGAGAACTCATATCTTTGGAAGATAACAGTGATGTCACAGGGTTGGCTATGTTTATT
  CTGAATCGCCTACTTTGGAATCCTGATATAGCAGCTGAGTATAGACACCCCACTGTTCCTCACCTGTAT
  AGAGATGGTCATGAAGAAGCTTTGTCCAAGTTTACATTGAAAAAGTTATTGTTGTTGGTCTGTTTTCTT
  GATTATGCTAAAATTTCCAGACTCATTGATCATGATCCTTGTCTCTTCTGTAAAGATGCCGAATTCAAG
  GCTAGTAAAGAAATCCTTTTGGCTTTTTCACGAGATTTCCTAAGTGGTGAAGGTGACCTTTCCCGTCAC
  CTTGGCTTATTGGGATTACCTGTTAACCATGTTCAGACACCATTTGATGAATTTGATTTTGCCGTTACA
  AATCTTGCCGTAGACTTGCAATGTGGAGTGCGCCTTGTGCGAACCATGGAACTTCTCACACAGAACTGG
  GACCTCTCAAAGAAACTCAGGATTCCGGCAATAAGTCGTCTTCAAAAGATGCACAATGTTGACATTGTT
  CTTCAAGTTCTTAAATCACGAGGAATTGAATTAAGTGATGAGCATGGAAATACAATTCTATCTAAGGAT
  ATTGTGGATAGGCACAGAGAAAAAACTCTCAGGTTGCTTTGGAAAATAGCGTTTGCTTTTCAGGTGGAT
  ATTTCCCTTAACTTAGATCAATTAAAGGAAGAAATTGCCTTTCTAAAACACACAAAGAGTATAAAGAAA
  ACAATATCTCTACTATCATGCCATTCTGATGATCTTATTAATAAGAAAAAAGGCAAAAGGGATAGTGGT
  TCCTTTGAACAATATAGTGAAAACATAAAGTTATTGATGGATTGGGTAAATGCTGTTTGTGCCTTCTAT
  AATAAAAAGGTGGAGAATTTTACAGTGTCTTTCTCAGACGGCCGTGTGTTATGTTACCTGATCCACCAT
  TACCATCCTTGCTATGTGCCATTTGACGCTATATGTCAGCGTACTACTCAAACTGTGGAATGTACGCAA
  ACTGGTTCAGTGGTATTAAATTCATCATCTGAATCTGATGACAGTTCTCTGGATATGTCTCTTAAAGCA
  TTTGATCATGAAAATACTTCAGAGCTATACAAAGAGCTCCTAGAAAATGAAAAGAAAAATTTTCACTTG
  GTTAGGTCTGCAGTTAGAGACCTTGGTGGAATACCTGCTATGATTAATCATTCAGATATGTCAAATACA
  ATTCCAGATGAAAAGGTGGTTATTACCTATTTGTCATTTCTTTGTGCAAGGCTTTTGGATCTTCGTAAA
  GAAATAAGAGCTGCTCGACTCATACAAACAACATGGAGAAAATATAAACTAAAAACAGATCTCAAACGC
  CATCAGGAGAGAGAGAAAGCTGCAAGAATTATTCAATTGGCTGTAATCAATTTTCTAGCAAAACAAAGA
  TTGAGAAAAAGAGTTAATGCAGCACTCGTCATTCAGAAATATTGGCGAAGAGTCTTAGCACAGAGAAAA
  TTATTAATGTTAAAAAAGGAAAAGCTGGAAAAAGTTCAAAATAAAGCAGCATCACTTATTCAGGCTATG
  TGGAGGAGATATAGAGCCAAGAAATATTTATGTAAAGTGAAAGCTGCCTGCAAGATTCAAGCCTGGTAT
  AGATGTTGGAGAGCACACAAAGAATATCTAGCTATATTAAAAGCTGTTAAAATTATTCAAGGTTGCTTC
  TATACCAAACTAGAGAGAACACGGTTTTTGAATGTGAGAGCATCAGCAATTATCATTCAGAGAAAATGG
  AGAGCTATACTTCCTGCAAAGATAGCTCATGAACACTTCTTAATGATAAAAAGACATCGAGCTGCTTGT
  TTGATCCAAGCACATTATAGAGGATATAAAGGAAGGCAGGTCTTTCTTCGGCAGAAATCTGCTGCTTTG
  ATCATACAAAAATATATACGAGCCAGGGAGGCTGGAAAGCATGAAAGGATAAAATATATTGAATTTAAA
  AAATCTACAGTTATCCTACAAGCACTGGTGCGTGGTTGGCTAGTACGAAAAAGATTTTTAGAACAGAGA
  GCCAAAATTCGACTTCTTCACTTCACTGCAGCTGCATATTATCACCTGAATGCTGTTAGAATTCAAAGA
  GCCTATAAACTTTACCTGGCTGTGAAGAATGCTAACAAGCAGGTTAATTCAGTCATCTGTATTCAGAGA
  TGGTTTCGAGCAAGATTACAAGAAAAGAGATTTATTCAGAAATATCATAGCATCAAAAAGATTGAGCAT
  GAAGGTCAAGAATGTCTGAGCCAGCGAAATAGGGCTGCATCAGTAATACAGAAAGCAGTGCGCCATTTT
  CTCCTCCGTAAAAAGCAGGAAAAATTCACTAGTGGAATCATTAAAATTCAGGCATTATGGAGAGGCTAT
  TCTTGGAGGAAGAAAAATGATTGTACAAAAATTAAAGCTATACGACTAAGTCTTCAAGTTGTTAATAGG
  GAGATTCGAGAAGAAAACAAACTCTACAAAAGAACTGCACTTGCACTTCATTACCTTTTGACATATAAG
  CACCTTTCTGCCATTCTTGAGGCCTTAAAACACCTAGAGGTAGTTACTAGATTGTCTCCACTTTGTTGT
  GAGAACATGGCCCAGAGTGGAGCAATTTCTAAAATATTTGTTTTGATCCGAAGTTGTAATCGCAGTATT
  CCTTGTATGGAAGTCATCAGATATGCTGTGCAAGTCTTGCTTAATGTATCTAAGTATGAGAAAACTACT
  TCAGCAGTTTATGATGTAGAAAATTGTATAGATATACTATTGGAGCTTTTGCAGATATACCGAGAAAAG
  CCTGGTAATAAAGTTGCAGACAAAGGCGGAAGCATTTTTACAAAAACTTGTTGTTTGTTGGCTATTTTA
  CTGAAGACAACAAATAGAGCCTCTGATGTACGAAGTAGGTCCAAAGTTGTTGACCGTATTTACAGTCTC
  TACAAACTTACAGCTCATAAACATAAAATGAATACTGAAAGAATACTTTACAAGCAAAAGAAGAATTCT
  TCTATAAGCATTCCTTTTATCCCAGAAACACCTGTAAGGACCAGAATAGTTTCAAGACTTAAGCCAGAT
  TGGGTTTTGAGAAGAGATAACATGGAAGAAATCACAAATCCCCTGCAAGCTATTCAAATGGTGATGGAT
  ACGCTTGGCATTCCTTATTAG
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001206846
• Insert Size: 5679 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001206846.1
• RefSeq Size: 6151 bp
• RefSeq ORF: 5679 bp
• Locus ID: 259266
• UniProt ID: Q8IZT6
• Cytogenetics: 1q31.3
• Protein Families: Druggable Genome
• MW: 217.8 kDa
• Gene Summary: This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1.