ASPM (NM_001206846) Human Tagged ORF Clone

CAT#: RC235345

ASPM (Myc-DDK tagged) - Homo sapiens asp (abnormal spindle) homolog, microcephaly associated (Drosophila) (ASPM), transcript variant 2

Product Images

Circular map for RC235345

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ASPM
• Synonyms: ASP; Calmbp1; MCPH5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC235345 representing NM_001206846
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAACCGGCGAGTGGGGCGAGGCTGCTGGGAAGTGAGCCCGACCGAGCGGAGGCCGCCCGCGGGGC
  TGCGGGGCCCCGCGGCCGAGGAGGAGGCGTCTTCCCCGCCGGTCCTGTCTCTCAGCCACTTCTGCAGGTC
  TCCTTTCCTTTGCTTCGGGGACGTTCTCCTGGGAGCCTCACGGACGCTGTCTCTGGCCCTAGACAACCCT
  AACGAGGAGGTGGCAGAAGTGAAGATCTCCCACTTCCCGGCCGCGGACCTGGGCTTCAGTGTGTCGCAGC
  GCTGTTTCGTGTTGCAGCCTAAAGAGAAAATTGTTATTTCTGTTAACTGGACACCACTCAAAGAAGGCCG
  AGTAAGAGAGATTATGACATTTCTTGTAAATGATGTTCTGAAACACCAAGCTATATTACTAGGAAATGCA
  GAAGAGCAGAAAAAGAAAAAGAGGAGTCTTTGGGATACCATTAAAAAGAAGAAAATTTCAGCCTCTACAA
  GTCACAACAGAAGGGTTTCAAATATTCAGAATGTTAATAAAACATTTAGTGTTTCCCAAAAAGTTGACAG
  AGTTAGGAGCCCACTACAAGCTTGTGAAAACTTGGCTATGAATGAAGGCGGTCCCCCAACAGAAAACAAT
  TCTTTAATACTTGAAGAAAATAAAATACCCATATCACCTATTAGCCCTGCTTTCAATGAATGCCATGGTG
  CAACTTGCTTGCCACTCTCTGTACGTCGATCTACTACCTACTCATCTCTTCATGCATCAGAAAATAGGGA
  ACTATTAAATGTACACAGTGCCAACGTTTCAAAAGTTTCTTTTAATGAGAAAGCTGTAACTGAAACTTCC
  TTTAATTCCGTAAATGTTAATGGCCAAAGAGGAGAGAATAGTAAACTTAGTCTTACCCCCAACTGTTCTT
  CAACTTTGAACATTACACAAAGCCAAATACATTTTCTAAGTCCAGATTCTTTTGTAAATAATAGTCATGG
  AGCTAATAATGAACTAGAATTAGTAACATGTCTTTCATCAGATATGTTTATGAAAGATAATTCACAGCCT
  GTGCATTTGGAATCAACAATTGCACATGAAATTTATCAGAAAATTTTAAGTCCAGATTCTTTCATAAAAG
  ATAATTATGGACTAAATCAGGATCTAGAATCAGAGTCAGTTAATCCTATTTTATCCCCTAATCAATTTTT
  AAAAGATAACATGGCATATATGTGTACATCTCAGCAAACATGTAAAGTACCATTATCAAATGAAAATTCT
  CAAGTCCCACAGTCTCCTGAAGATTGGAGAAAAAGTGAAGTTTCGCCACGTATTCCTGAATGTCAGGGTT
  CAAAATCTCCCAAAGCTATTTTTGAAGAACTAGTAGAAATGAAGTCAAATTACTACAGTTTTATAAAACA
  AAATAATCCTAAATTTTCTGCAGTTCAGGATATTTCTAGTCATAGCCACAATAAACAACCTAAGAGACGT
  CCAATACTTTCTGCCACTGTTACTAAAAGGAAGGCCACCTGTACCAGAGAAAACCAAACTGAGATTAATA
  AACCAAAAGCAAAAAGATGTCTCAACAGTGCAGTGGGTGAACATGAAAAAGTAATAAATAATCAAAAGGA
  AAAAGAAGATTTTCATTCTTATCTTCCAATTATAGATCCAATATTAAGTAAATCTAAGAGTTATAAAAAC
  GAGGTAACACCCTCTTCGACAACAGCTTCAGTTGCTCGGAAAAGAAAGAGCGATGGAAGCATGGAAGATG
  CAAATGTGAGAGTTGCAATTACAGAACATACAGAAGTGCGAGAAATCAAAAGAATCCATTTTTCTCCCTC
  AGAGCCTAAAACATCAGCTGTTAAGAAAACAAAAAATGTGACAACACCCATCTCAAAACGTATTAGCAAC
  AGAGAGAAATTAAACCTGAAGAAGAAAACTGATTTATCAATATTCAGAACTCCAATTTCTAAAACAAACA
  AAAGGACAAAACCCATTATCGCTGTGGCACAGTCCAGTTTGACCTTCATAAAACCATTAAAAACAGATAT
  TCCCAGACACCCGATGCCATTTGCTGCAAAAAACATGTTTTATGATGAACGCTGGAAGGAAAAGCAGGAA
  CAGGGCTTCACTTGGTGGTTAAATTTTATATTAACCCCTGATGACTTCACTGTAAAAACAAATATTTCTG
  AAGTAAATGCTGCTACTCTTCTTTTGGGAATAGAGAATCAACATAAAATAAGTGTTCCTAGAGCACCTAC
  AAAAGAGGAAATGTCTCTCAGAGCTTATACTGCTCGGTGTAGGTTAAACAGACTACGTCGTGCAGCATGC
  CGTTTGTTTACTTCTGAAAAAATGGTTAAAGCTATTAAAAAGCTTGAAATTGAAATTGAAGCTAGGCGGT
  TAATTGTTCGAAAAGATAGACACCTATGGAAAGATGTGGGAGAACGTCAGAAAGTCCTGAATTGGCTGTT
  GTCCTACAATCCTTTGTGGCTTCGAATTGGTCTAGAGACAACTTATGGAGAACTCATATCTTTGGAAGAT
  AACAGTGATGTCACAGGGTTGGCTATGTTTATTCTGAATCGCCTACTTTGGAATCCTGATATAGCAGCTG
  AGTATAGACACCCCACTGTTCCTCACCTGTATAGAGATGGTCATGAAGAAGCTTTGTCCAAGTTTACATT
  GAAAAAGTTATTGTTGTTGGTCTGTTTTCTTGATTATGCTAAAATTTCCAGACTCATTGATCATGATCCT
  TGTCTCTTCTGTAAAGATGCCGAATTCAAGGCTAGTAAAGAAATCCTTTTGGCTTTTTCACGAGATTTCC
  TAAGTGGTGAAGGTGACCTTTCCCGTCACCTTGGCTTATTGGGATTACCTGTTAACCATGTTCAGACACC
  ATTTGATGAATTTGATTTTGCCGTTACAAATCTTGCCGTAGACTTGCAATGTGGAGTGCGCCTTGTGCGA
  ACCATGGAACTTCTCACACAGAACTGGGACCTCTCAAAGAAACTCAGGATTCCGGCAATAAGTCGTCTTC
  AAAAGATGCACAATGTTGACATTGTTCTTCAAGTTCTTAAATCACGAGGAATTGAATTAAGTGATGAGCA
  TGGAAATACAATTCTATCTAAGGATATTGTGGATAGGCACAGAGAAAAAACTCTCAGGTTGCTTTGGAAA
  ATAGCGTTTGCTTTTCAGGTGGATATTTCCCTTAACTTAGATCAATTAAAGGAAGAAATTGCCTTTCTAA
  AACACACAAAGAGTATAAAGAAAACAATATCTCTACTATCATGCCATTCTGATGATCTTATTAATAAGAA
  AAAAGGCAAAAGGGATAGTGGTTCCTTTGAACAATATAGTGAAAACATAAAGTTATTGATGGATTGGGTA
  AATGCTGTTTGTGCCTTCTATAATAAAAAGGTGGAGAATTTTACAGTGTCTTTCTCAGACGGCCGTGTGT
  TATGTTACCTGATCCACCATTACCATCCTTGCTATGTGCCATTTGACGCTATATGTCAGCGTACTACTCA
  AACTGTGGAATGTACGCAAACTGGTTCAGTGGTATTAAATTCATCATCTGAATCTGATGACAGTTCTCTG
  GATATGTCTCTTAAAGCATTTGATCATGAAAATACTTCAGAGCTATACAAAGAGCTCCTAGAAAATGAAA
  AGAAAAATTTTCACTTGGTTAGGTCTGCAGTTAGAGACCTTGGTGGAATACCTGCTATGATTAATCATTC
  AGATATGTCAAATACAATTCCAGATGAAAAGGTGGTTATTACCTATTTGTCATTTCTTTGTGCAAGGCTT
  TTGGATCTTCGTAAAGAAATAAGAGCTGCTCGACTCATACAAACAACATGGAGAAAATATAAACTAAAAA
  CAGATCTCAAACGCCATCAGGAGAGAGAGAAAGCTGCAAGAATTATTCAATTGGCTGTAATCAATTTTCT
  AGCAAAACAAAGATTGAGAAAAAGAGTTAATGCAGCACTCGTCATTCAGAAATATTGGCGAAGAGTCTTA
  GCACAGAGAAAATTATTAATGTTAAAAAAGGAAAAGCTGGAAAAAGTTCAAAATAAAGCAGCATCACTTA
  TTCAGGCTATGTGGAGGAGATATAGAGCCAAGAAATATTTATGTAAAGTGAAAGCTGCCTGCAAGATTCA
  AGCCTGGTATAGATGTTGGAGAGCACACAAAGAATATCTAGCTATATTAAAAGCTGTTAAAATTATTCAA
  GGTTGCTTCTATACCAAACTAGAGAGAACACGGTTTTTGAATGTGAGAGCATCAGCAATTATCATTCAGA
  GAAAATGGAGAGCTATACTTCCTGCAAAGATAGCTCATGAACACTTCTTAATGATAAAAAGACATCGAGC
  TGCTTGTTTGATCCAAGCACATTATAGAGGATATAAAGGAAGGCAGGTCTTTCTTCGGCAGAAATCTGCT
  GCTTTGATCATACAAAAATATATACGAGCCAGGGAGGCTGGAAAGCATGAAAGGATAAAATATATTGAAT
  TTAAAAAATCTACAGTTATCCTACAAGCACTGGTGCGTGGTTGGCTAGTACGAAAAAGATTTTTAGAACA
  GAGAGCCAAAATTCGACTTCTTCACTTCACTGCAGCTGCATATTATCACCTGAATGCTGTTAGAATTCAA
  AGAGCCTATAAACTTTACCTGGCTGTGAAGAATGCTAACAAGCAGGTTAATTCAGTCATCTGTATTCAGA
  GATGGTTTCGAGCAAGATTACAAGAAAAGAGATTTATTCAGAAATATCATAGCATCAAAAAGATTGAGCA
  TGAAGGTCAAGAATGTCTGAGCCAGCGAAATAGGGCTGCATCAGTAATACAGAAAGCAGTGCGCCATTTT
  CTCCTCCGTAAAAAGCAGGAAAAATTCACTAGTGGAATCATTAAAATTCAGGCATTATGGAGAGGCTATT
  CTTGGAGGAAGAAAAATGATTGTACAAAAATTAAAGCTATACGACTAAGTCTTCAAGTTGTTAATAGGGA
  GATTCGAGAAGAAAACAAACTCTACAAAAGAACTGCACTTGCACTTCATTACCTTTTGACATATAAGCAC
  CTTTCTGCCATTCTTGAGGCCTTAAAACACCTAGAGGTAGTTACTAGATTGTCTCCACTTTGTTGTGAGA
  ACATGGCCCAGAGTGGAGCAATTTCTAAAATATTTGTTTTGATCCGAAGTTGTAATCGCAGTATTCCTTG
  TATGGAAGTCATCAGATATGCTGTGCAAGTCTTGCTTAATGTATCTAAGTATGAGAAAACTACTTCAGCA
  GTTTATGATGTAGAAAATTGTATAGATATACTATTGGAGCTTTTGCAGATATACCGAGAAAAGCCTGGTA
  ATAAAGTTGCAGACAAAGGCGGAAGCATTTTTACAAAAACTTGTTGTTTGTTGGCTATTTTACTGAAGAC
  AACAAATAGAGCCTCTGATGTACGAAGTAGGTCCAAAGTTGTTGACCGTATTTACAGTCTCTACAAACTT
  ACAGCTCATAAACATAAAATGAATACTGAAAGAATACTTTACAAGCAAAAGAAGAATTCTTCTATAAGCA
  TTCCTTTTATCCCAGAAACACCTGTAAGGACCAGAATAGTTTCAAGACTTAAGCCAGATTGGGTTTTGAG
  AAGAGATAACATGGAAGAAATCACAAATCCCCTGCAAGCTATTCAAATGGTGATGGATACGCTTGGCATT
  CCTTAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC235345 representing NM_001206846
  Red=Cloning site Green=Tags(s)
  
  MANRRVGRGCWEVSPTERRPPAGLRGPAAEEEASSPPVLSLSHFCRSPFLCFGDVLLGASRTLSLALDNP
  NEEVAEVKISHFPAADLGFSVSQRCFVLQPKEKIVISVNWTPLKEGRVREIMTFLVNDVLKHQAILLGNA
  EEQKKKKRSLWDTIKKKKISASTSHNRRVSNIQNVNKTFSVSQKVDRVRSPLQACENLAMNEGGPPTENN
  SLILEENKIPISPISPAFNECHGATCLPLSVRRSTTYSSLHASENRELLNVHSANVSKVSFNEKAVTETS
  FNSVNVNGQRGENSKLSLTPNCSSTLNITQSQIHFLSPDSFVNNSHGANNELELVTCLSSDMFMKDNSQP
  VHLESTIAHEIYQKILSPDSFIKDNYGLNQDLESESVNPILSPNQFLKDNMAYMCTSQQTCKVPLSNENS
  QVPQSPEDWRKSEVSPRIPECQGSKSPKAIFEELVEMKSNYYSFIKQNNPKFSAVQDISSHSHNKQPKRR
  PILSATVTKRKATCTRENQTEINKPKAKRCLNSAVGEHEKVINNQKEKEDFHSYLPIIDPILSKSKSYKN
  EVTPSSTTASVARKRKSDGSMEDANVRVAITEHTEVREIKRIHFSPSEPKTSAVKKTKNVTTPISKRISN
  REKLNLKKKTDLSIFRTPISKTNKRTKPIIAVAQSSLTFIKPLKTDIPRHPMPFAAKNMFYDERWKEKQE
  QGFTWWLNFILTPDDFTVKTNISEVNAATLLLGIENQHKISVPRAPTKEEMSLRAYTARCRLNRLRRAAC
  RLFTSEKMVKAIKKLEIEIEARRLIVRKDRHLWKDVGERQKVLNWLLSYNPLWLRIGLETTYGELISLED
  NSDVTGLAMFILNRLLWNPDIAAEYRHPTVPHLYRDGHEEALSKFTLKKLLLLVCFLDYAKISRLIDHDP
  CLFCKDAEFKASKEILLAFSRDFLSGEGDLSRHLGLLGLPVNHVQTPFDEFDFAVTNLAVDLQCGVRLVR
  TMELLTQNWDLSKKLRIPAISRLQKMHNVDIVLQVLKSRGIELSDEHGNTILSKDIVDRHREKTLRLLWK
  IAFAFQVDISLNLDQLKEEIAFLKHTKSIKKTISLLSCHSDDLINKKKGKRDSGSFEQYSENIKLLMDWV
  NAVCAFYNKKVENFTVSFSDGRVLCYLIHHYHPCYVPFDAICQRTTQTVECTQTGSVVLNSSSESDDSSL
  DMSLKAFDHENTSELYKELLENEKKNFHLVRSAVRDLGGIPAMINHSDMSNTIPDEKVVITYLSFLCARL
  LDLRKEIRAARLIQTTWRKYKLKTDLKRHQEREKAARIIQLAVINFLAKQRLRKRVNAALVIQKYWRRVL
  AQRKLLMLKKEKLEKVQNKAASLIQAMWRRYRAKKYLCKVKAACKIQAWYRCWRAHKEYLAILKAVKIIQ
  GCFYTKLERTRFLNVRASAIIIQRKWRAILPAKIAHEHFLMIKRHRAACLIQAHYRGYKGRQVFLRQKSA
  ALIIQKYIRAREAGKHERIKYIEFKKSTVILQALVRGWLVRKRFLEQRAKIRLLHFTAAAYYHLNAVRIQ
  RAYKLYLAVKNANKQVNSVICIQRWFRARLQEKRFIQKYHSIKKIEHEGQECLSQRNRAASVIQKAVRHF
  LLRKKQEKFTSGIIKIQALWRGYSWRKKNDCTKIKAIRLSLQVVNREIREENKLYKRTALALHYLLTYKH
  LSAILEALKHLEVVTRLSPLCCENMAQSGAISKIFVLIRSCNRSIPCMEVIRYAVQVLLNVSKYEKTTSA
  VYDVENCIDILLELLQIYREKPGNKVADKGGSIFTKTCCLLAILLKTTNRASDVRSRSKVVDRIYSLYKL
  TAHKHKMNTERILYKQKKNSSISIPFIPETPVRTRIVSRLKPDWVLRRDNMEEITNPLQAIQMVMDTLGI
  PY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001206846
• ORF Size: 5676 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001206846.1, NP_001193775.1
• RefSeq Size: 6151 bp
• RefSeq ORF: 5679 bp
• Locus ID: 259266
• UniProt ID: Q8IZT6
• Cytogenetics: 1q31.3
• Protein Families: Druggable Genome
• MW: 218.2 kDa
• Gene Summary: This gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts. Studies in mouse also suggest a role of this gene in mitotic spindle regulation, with a preferential role in regulating neurogenesis. Mutations in this gene are associated with microcephaly primary type 5. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2011]