GPR35 (NM_001195382) Human Untagged Clone

CAT#: SC329454

GPR35 (untagged) - Homo sapiens G protein-coupled receptor 35 (GPR35), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GPR35
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC329454 representing NM_001195382.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGAGTGGTTCCCGGGCTGTCCCCACTCCACACCGTGGCAGTGAAGAGCTGCTGAAGTACATGCTT
  CATAGTCCTTGCGTCTCTCTGACCATGAATGGCACCTACAACACCTGTGGCTCCAGCGACCTCACCTGG
  CCCCCAGCGATCAAGCTGGGCTTCTACGCCTACTTGGGCGTCCTGCTGGTGCTAGGCCTGCTGCTCAAC
  AGCCTGGCGCTCTGGGTGTTCTGCTGCCGCATGCAGCAGTGGACGGAGACCCGCATCTACATGACCAAC
  CTGGCGGTGGCCGACCTCTGCCTGCTGTGCACCTTGCCCTTCGTGCTGCACTCCCTGCGAGACACCTCA
  GACACGCCGCTGTGCCAGCTCTCCCAGGGCATCTACCTGACCAACAGGTACATGAGCATCAGCCTGGTC
  ACGGCCATCGCCGTGGACCGCTATGTGGCCGTGCGGCACCCGCTGCGTGCCCGCGGGCTGCGGTCCCCC
  AGGCAGGCTGCGGCCGTGTGCGCGGTCCTCTGGGTGCTGGTCATCGGCTCCCTGGTGGCTCGCTGGCTC
  CTGGGGATTCAGGAGGGCGGCTTCTGCTTCAGGAGCACCCGGCACAATTTCAACTCCATGGCGTTCCCG
  CTGCTGGGATTCTACCTGCCCCTGGCCGTGGTGGTCTTCTGCTCCCTGAAGGTGGTGACTGCCCTGGCC
  CAGAGGCCACCCACCGACGTGGGGCAGGCAGAGGCCACCCGCAAGGCTGCCCGCATGGTCTGGGCCAAC
  CTCCTGGTGTTCGTGGTCTGCTTCCTGCCCCTGCACGTGGGGCTGACAGTGCGCCTCGCAGTGGGCTGG
  AACGCCTGTGCCCTCCTGGAGACGATCCGTCGCGCCCTGTACATAACCAGCAAGCTCTCAGATGCCAAC
  TGCTGCCTGGACGCCATCTGCTACTACTACATGGCCAAGGAGTTCCAGGAGGCGTCTGCACTGGCCGTG
  GCTCCCAGTGCTAAGGCCCACAAAAGCCAGGACTCTCTGTGCGTGACCCTCGCCTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001195382
• Insert Size: 1023 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001195382.1
• RefSeq Size: 2179 bp
• RefSeq ORF: 1023 bp
• Locus ID: 2859
• UniProt ID: Q9HC97
• Cytogenetics: 2q37.3
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 37.5 kDa
• Gene Summary: Acts as a receptor for kynurenic acid, an intermediate in the tryptophan metabolic pathway. The activity of this receptor is mediated by G-proteins that elicit calcium mobilization and inositol phosphate production through G(qi/o) proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and uses an alternate start codon, compared to variant 1. The resulting isoform (b, also known as GPR35b) is longer at the N-terminus, compared to isoform a. Both variants 2 and 3 encode the same isoform (b).