MCF2 (NM_001171879) Human Untagged Clone

CAT#: SC329225

MCF2 (untagged)-Human MCF.2 cell line derived transforming sequence (MCF2) transcript variant 6

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCF2
• Synonyms: ARHGEF21; DBL
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001171879, the custom clone sequence may differ by one or more nucleotides
  ATGGCAGAAGCAAATCCCCGGAGAGGCAAGATGAGGTTCAGAAGGAATGCGGCTTCCTTC
  CCTGGGAACTTGCACTTGGTTTTGGTTTTACGTCCTACCAGCTTTCTTCAACGAACGTTC
  ACAGACATTGGATTTTGGTTTAGTCAGGAGGATTTTATGCTTAAATTACCAGTTGTTATG
  CTGAGCTCAGTTAGTGATTTGCTGACATACATTGATGACAAGCAATTAACCCCTGAGTTA
  GGCGGCACCTTGCAGTACTGCCACAGTGAATGGATCATCTTCAGAAATGCTATAGAAAAT
  TTTGCCCTCACAGTGAAAGAAATGGCTCAGATGTTACAGTCCTTTGGAACTGAACTGGCT
  GAGACAGAACTACCAGATGATATTCCCTCAATAGAAGAAATTCTGGCAATTCGTGCTGAA
  AGGTATCATCTGTTGAAGAATGATATTACAGCTGTAACCAAAGAAGGAAAAATTCTGCTA
  ACAAATCTGGAAGTGCCTGACACTGAAGGAGCTGTCAGTTCAAGACTAGAATGTCATCGG
  CAAATAAGTGGTGACTGGCAAACTATTAATAAGTTGCTGACTCAAGTACATGATATGGAA
  ACAGCTTTTGATGGATTTTGGGAAAAACATCAATTAAAAATGGAGCAGTATCTGCAACTA
  TGGAAGTTTGAGCAGGATTTTCAACAGCTTGTGACTGAAGTTGAATTTCTATTAAACCAA
  CAAGCAGAACTGGCTGATGTAACAGGGACTATAGCTCAAGTAAAACAAAAAATAAAAAAA
  TTGGAAAACTTAGATGAAAATTCTCAGGAGCTATTATCAAAGGCCCAGTTTGTGATATTA
  CATGGACACAAGCTTGCAGCAAATCACCATTATGCACTTGATTTAATCTGCCAGAGGTGC
  AATGAGCTACGTTACCTTTCTGATATTTTGGTTAATGAGATAAAAGCAAAACGGATACAA
  CTCAGCAGGACCTTCAAAATGCATAAACTCCTACAGCAGGCTCGTCAATGCTGTGATGAA
  GGGGAATGTCTTCTAGCTAATCAGGAAATAGATAAGTTTCAGTCTAAAGAAGATGCTCAG
  AAAGCTCTCCAAGACATTGAAAATTTTCTTGAAATGGCTCTACCCTTTATAAATTATGAA
  CCTGAAACACTGCAGTATGAATTTGATGTAATATTATCTCCTGAGCTTAAGGTTCAAATG
  AAGACTATACAACTCAAGCTTGAAAACATTCGAAGTATATTTGAGAACCAGCAGGCTGGT
  TTCAGGAACCTGGCAGATAAGCATGTGAGGCCAATCCAATTTGTGGTACCCACACCTGAA
  AATTTGGTCACATCTGGGACACCATTTTTTTCATCTAAACAAGTGGGAGTTGGATACTCT
  TTCTTTCAAGCATGTAAACTTTTTTCAAAAGGGAAGAAGACTTGGAGACAAAATCAGAGC
  AACTTAAAAATTGAAGTGGTGCCTGATTGTCAGGAGAAGAGAAGTTCTGGTCCATCCTCC
  AGTTTGGACAATGGCAATAGCTTGGATGTTTTAAAGAACCACGTACTAAATGAACTGATA
  CAGACTGAGAGAGTTTATGTTCGAGAACTGTATACTGTTTTGTTGGGTTATAGAGCGGAG
  ATGGATAATCCAGAGATGTTTGATCTTATGCCACCTCTCCTGAGAAATAAAAAGGACATT
  CTCTTTGGAAACATGGCAGAAATATATGAATTCCATAACGACATTTTCTTGAGCAGCCTG
  GAAAATTGTGCTCATGCTCCAGAAAGAGTGGGACCTTGTTTCCTGGAAAGGAAGGATGAT
  TTTCAGATGTATGCAAAATATTGTCAGAATAAGCCCAGATCAGAAACAATTTGGAGGAAG
  TATTCAGAATGCGCATTTTTCCAGGAATGTCAAAGAAAGTTAAAACACAGACTTAGACTG
  GATTCCTATTTACTCAAACCAGTGCAACGAATCACTAAATATCAGTTATTGTTGAAGGAG
  CTATTAAAATATAGCAAAGACTGTGAAGGATCTGCTCTGTTGAAGAAGGCACTCGATGCA
  ATGCTGGATTTACTGAAGTCAGTTAATGATTCTATGCATCAGATTGCAATAAATGGCTAT
  ATTGGAAACTTAAATGAACTGGGCAAGATGATAATGCAAGGTGGATTCAGCGTTTGGATA
  GGGCACAAGAAAGGTGCTACAAAAATGAAGGATTTGGCTAGATTCAAACCAATGCAGCGA
  CACCTTTTCTTGTATGAAAAAGCCATTGTTTTTTGCAAAAGGCGTGTTGAAAGTGGAGAA
  GGCTCTGACAGATACCCGTCATACAGTTTTAAACACTGTTGGAAAATGGATGAAGTTGGA
  ATCACTGAATATGTAAAAGGTGATAACCGCAAGTTTGAAATCTGGTATGGTGAAAAGGAA
  GAAGTTTATATTGTCCAGGCTTCTAATGTAGATGTGAAGATGACGTGGCTAAAAGAAATA
  AGAAATATTTTGTTGAAGCAGCAGGAACTTTTGACAGTTAAAAAAAGAAAGCAACAGGAT
  CAATTAACAGAACGGGATAAGTTTCAGATTTCTCTTCAGCAGAATGATGAAAAGCAACAG
  GGAGCTTTTATAAGTACTGAGGAAACTGAATTGGAACACACCAGCACTGTGGTGGAGGTC
  TGTGAGGCAATTGCGTCAGTTCAGGCAGAAGCAAATACAGTTTGGACTGAGGCATCACAA
  TCTGCAGAAATCTCTGAAGAACCTGCGGAATGGTCAAGCAACTATTTCTACCCTACTTAT
  GATGAAAATGAAGAAGAAAATAGGCCCCTCATGAGACCTGTGTCGGAGATGGCTCTCCTA
  TATTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001171879
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001171879.1, NP_001165350.1
• RefSeq Size: 3849 bp
• RefSeq ORF: 2826 bp
• Locus ID: 4168
• UniProt ID: P10911
• Cytogenetics: Xq27.1
• Protein Families: Druggable Genome
• Gene Summary: The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.[provided by RefSeq, Jan 2010]
  Transcript Variant: This variant (6) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (f) has a shorter and distinct N-terminus compared to isoform c.