MCF2 (NM_001171879) Human Tagged ORF Clone

CAT#: RG230587

MCF2 (tGFP-tagged) - Human MCF.2 cell line derived transforming sequence (MCF2), transcript variant 6

Product Images

Circular map for RG230587

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: MCF2
• Synonyms: ARHGEF21; DBL
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG230587 representing NM_001171879
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAGCAAATCCCCGGAGAGGCAAGATGAGGTTCAGAAGGAATGCGGCTTCCTTCCCTGGGAACT
  TGCACTTGGTTTTGGTTTTACGTCCTACCAGCTTTCTTCAACGAACGTTCACAGACATTGGATTTTGGTT
  TAGTCAGGAGGATTTTATGCTTAAATTACCAGTTGTTATGCTGAGCTCAGTTAGTGATTTGCTGACATAC
  ATTGATGACAAGCAATTAACCCCTGAGTTAGGCGGCACCTTGCAGTACTGCCACAGTGAATGGATCATCT
  TCAGAAATGCTATAGAAAATTTTGCCCTCACAGTGAAAGAAATGGCTCAGATGTTACAGTCCTTTGGAAC
  TGAACTGGCTGAGACAGAACTACCAGATGATATTCCCTCAATAGAAGAAATTCTGGCAATTCGTGCTGAA
  AGGTATCATCTGTTGAAGAATGATATTACAGCTGTAACCAAAGAAGGAAAAATTCTGCTAACAAATCTGG
  AAGTGCCTGACACTGAAGGAGCTGTCAGTTCAAGACTAGAATGTCATCGGCAAATAAGTGGTGACTGGCA
  AACTATTAATAAGTTGCTGACTCAAGTACATGATATGGAAACAGCTTTTGATGGATTTTGGGAAAAACAT
  CAATTAAAAATGGAGCAGTATCTGCAACTATGGAAGTTTGAGCAGGATTTTCAACAGCTTGTGACTGAAG
  TTGAATTTCTATTAAACCAACAAGCAGAACTGGCTGATGTAACAGGGACTATAGCTCAAGTAAAACAAAA
  AATAAAAAAATTGGAAAACTTAGATGAAAATTCTCAGGAGCTATTATCAAAGGCCCAGTTTGTGATATTA
  CATGGACACAAGCTTGCAGCAAATCACCATTATGCACTTGATTTAATCTGCCAGAGGTGCAATGAGCTAC
  GTTACCTTTCTGATATTTTGGTTAATGAGATAAAAGCAAAACGGATACAACTCAGCAGGACCTTCAAAAT
  GCATAAACTCCTACAGCAGGCTCGTCAATGCTGTGATGAAGGGGAATGTCTTCTAGCTAATCAGGAAATA
  GATAAGTTTCAGTCTAAAGAAGATGCTCAGAAAGCTCTCCAAGACATTGAAAATTTTCTTGAAATGGCTC
  TACCCTTTATAAATTATGAACCTGAAACACTGCAGTATGAATTTGATGTAATATTATCTCCTGAGCTTAA
  GGTTCAAATGAAGACTATACAACTCAAGCTTGAAAACATTCGAAGTATATTTGAGAACCAGCAGGCTGGT
  TTCAGGAACCTGGCAGATAAGCATGTGAGGCCAATCCAATTTGTGGTACCCACACCTGAAAATTTGGTCA
  CATCTGGGACACCATTTTTTTCATCTAAACAAGTGGGAGTTGGATACTCTTTCTTTCAAGCATGTAAACT
  TTTTTCAAAAGGGAAGAAGACTTGGAGACAAAATCAGAGCAACTTAAAAATTGAAGTGGTGCCTGATTGT
  CAGGAGAAGAGAAGTTCTGGTCCATCCTCCAGTTTGGACAATGGCAATAGCTTGGATGTTTTAAAGAACC
  ACGTACTAAATGAACTGATACAGACTGAGAGAGTTTATGTTCGAGAACTGTATACTGTTTTGTTGGGTTA
  TAGAGCGGAGATGGATAATCCAGAGATGTTTGATCTTATGCCACCTCTCCTGAGAAATAAAAAGGACATT
  CTCTTTGGAAACATGGCAGAAATATATGAATTCCATAACGACATTTTCTTGAGCAGCCTGGAAAATTGTG
  CTCATGCTCCAGAAAGAGTGGGACCTTGTTTCCTGGAAAGGAAGGATGATTTTCAGATGTATGCAAAATA
  TTGTCAGAATAAGCCCAGATCAGAAACAATTTGGAGGAAGTATTCAGAATGCGCATTTTTCCAGGAATGT
  CAAAGAAAGTTAAAACACAGACTTAGACTGGATTCCTATTTACTCAAACCAGTGCAACGAATCACTAAAT
  ATCAGTTATTGTTGAAGGAGCTATTAAAATATAGCAAAGACTGTGAAGGATCTGCTCTGTTGAAGAAGGC
  ACTCGATGCAATGCTGGATTTACTGAAGTCAGTTAATGATTCTATGCATCAGATTGCAATAAATGGCTAT
  ATTGGAAACTTAAATGAACTGGGCAAGATGATAATGCAAGGTGGATTCAGCGTTTGGATAGGGCACAAGA
  AAGGTGCTACAAAAATGAAGGATTTGGCTAGATTCAAACCAATGCAGCGACACCTTTTCTTGTATGAAAA
  AGCCATTGTTTTTTGCAAAAGGCGTGTTGAAAGTGGAGAAGGCTCTGACAGATACCCGTCATACAGTTTT
  AAACACTGTTGGAAAATGGATGAAGTTGGAATCACTGAATATGTAAAAGGTGATAACCGCAAGTTTGAAA
  TCTGGTATGGTGAAAAGGAAGAAGTTTATATTGTCCAGGCTTCTAATGTAGATGTGAAGATGACGTGGCT
  AAAAGAAATAAGAAATATTTTGTTGAAGCAGCAGGAACTTTTGACAGTTAAAAAAAGAAAGCAACAGGAT
  CAATTAACAGAACGGGATAAGTTTCAGATTTCTCTTCAGCAGAATGATGAAAAGCAACAGGGAGCTTTTA
  TAAGTACTGAGGAAACTGAATTGGAACACACCAGCACTGTGGTGGAGGTCTGTGAGGCAATTGCGTCAGT
  TCAGGCAGAAGCAAATACAGTTTGGACTGAGGCATCACAATCTGCAGAAATCTCTGAAGAACCTGCGGAA
  TGGTCAAGCAACTATTTCTACCCTACTTATGATGAAAATGAAGAAGAAAATAGGCCCCTCATGAGACCTG
  TGTCGGAGATGGCTCTCCTATAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG230587 representing NM_001171879
  Red=Cloning site Green=Tags(s)
  
  MAEANPRRGKMRFRRNAASFPGNLHLVLVLRPTSFLQRTFTDIGFWFSQEDFMLKLPVVMLSSVSDLLTY
  IDDKQLTPELGGTLQYCHSEWIIFRNAIENFALTVKEMAQMLQSFGTELAETELPDDIPSIEEILAIRAE
  RYHLLKNDITAVTKEGKILLTNLEVPDTEGAVSSRLECHRQISGDWQTINKLLTQVHDMETAFDGFWEKH
  QLKMEQYLQLWKFEQDFQQLVTEVEFLLNQQAELADVTGTIAQVKQKIKKLENLDENSQELLSKAQFVIL
  HGHKLAANHHYALDLICQRCNELRYLSDILVNEIKAKRIQLSRTFKMHKLLQQARQCCDEGECLLANQEI
  DKFQSKEDAQKALQDIENFLEMALPFINYEPETLQYEFDVILSPELKVQMKTIQLKLENIRSIFENQQAG
  FRNLADKHVRPIQFVVPTPENLVTSGTPFFSSKQVGVGYSFFQACKLFSKGKKTWRQNQSNLKIEVVPDC
  QEKRSSGPSSSLDNGNSLDVLKNHVLNELIQTERVYVRELYTVLLGYRAEMDNPEMFDLMPPLLRNKKDI
  LFGNMAEIYEFHNDIFLSSLENCAHAPERVGPCFLERKDDFQMYAKYCQNKPRSETIWRKYSECAFFQEC
  QRKLKHRLRLDSYLLKPVQRITKYQLLLKELLKYSKDCEGSALLKKALDAMLDLLKSVNDSMHQIAINGY
  IGNLNELGKMIMQGGFSVWIGHKKGATKMKDLARFKPMQRHLFLYEKAIVFCKRRVESGEGSDRYPSYSF
  KHCWKMDEVGITEYVKGDNRKFEIWYGEKEEVYIVQASNVDVKMTWLKEIRNILLKQQELLTVKKRKQQD
  QLTERDKFQISLQQNDEKQQGAFISTEETELEHTSTVVEVCEAIASVQAEANTVWTEASQSAEISEEPAE
  WSSNYFYPTYDENEEENRPLMRPVSEMALLY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001171879
• ORF Size: 2823 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001171879.1, NP_001165350.1
• RefSeq Size: 3849 bp
• RefSeq ORF: 2826 bp
• Locus ID: 4168
• UniProt ID: P10911
• Cytogenetics: Xq27.1
• Protein Families: Druggable Genome
• Gene Summary: The oncogenic protein encoded by this gene is a guanine nucleotide exchange factor (GEF) that exerts control over some members of the Rho family of small GTPases. Several transcript variants encoding different isoforms have been found for this gene. These isoforms exhibit different expression patterns and varying levels of GEF activity.[provided by RefSeq, Jan 2010]