USP20 (NM_001110303) Human Untagged Clone

CAT#: SC328028

USP20 (untagged)-Human ubiquitin specific peptidase 20 (USP20) transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP20
• Synonyms: hVDU2; LSFR3A; VDU2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001110303, the custom clone sequence may differ by one or more nucleotides
  ATGGGGGACTCCAGGGACCTTTGCCCTCACCTTGACTCCATAGGAGAGGTGACCAAAGAG
  GACTTGCTGCTCAAATCTAAGGGAACCTGTCAGTCGTGTGGGGTCACCGGACCAAACCTA
  TGGGCCTGTCTGCAGGTTGCCTGCCCCTATGTTGGCTGCGGAGAATCCTTTGCTGACCAC
  AGCACCATTCATGCACAGGCAAAAAAGCACAACTTGACCGTGAACCTGACCACGTTCCGA
  CTGTGGTGTTACGCCTGTGAGAAGGAGGTATTCCTGGAGCAGCGGCTGGCAGCCCCTCTG
  CTGGGCTCCTCTTCCAAGTTCTCTGAACAGGACTCCCCGCCACCCTCCCACCCTCTGAAA
  GCTGTTCCTATTGCTGTGGCTGATGAAGGAGAGTCTGAGTCAGAGGACGATGACCTGAAA
  CCTCGAGGCCTCACGGGCATGAAGAACCTCGGGAACTCCTGCTACATGAACGCTGCCCTG
  CAGGCCCTGTCCAATTGCCCGCCGCTGACTCAGTTCTTCTTGGAGTGTGGCGGCCTGGTG
  CGCACAGATAAGAAGCCAGCCCTGTGCAAGAGCTACCAGAAGCTGGTCTCTGAGGTCTGG
  CATAAGAAACGGCCAAGCTACGTGGTCCCCACCAGTCTGTCTCATGGGATCAAGTTGGTC
  AACCCAATGTTCCGAGGCTATGCCCAGCAGGACACCCAAGAGTTCCTTCGCTGCCTGATG
  GACCAGCTGCACGAGGAGCTCAAGGAGCCGGTGGTGGCCACGGTGGCGCTGACGGAGGCT
  CGGGACTCAGATTCGAGTGACACGGATGAGAAACGGGAGGGTGACCGGAGCCCATCAGAA
  GATGAGTTCTTGTCCTGTGACTCGAGCAGTGACCGGGGTGAGGGTGACGGGCAGGGGCGT
  GGCGGGGGCAGCTCGCAGGCCGAGACGGAGCTGCTGATCCCAGATGAGGCGGGCCGAGCC
  ATCTCTGAGAAGGAGCGGATGAAGGACCGCAAGTTCTCCTGGGGCCAGCAGCGTACAAAC
  TCGGAGCAAGTGGACGAGGACGCTGATGTGGACACTGCCATGGCTGCCCTTGACGACCAG
  CCCGCGGAGGCCCAGCCCCCGTCACCACGGTCCTCCAGCCCCTGCCGGACGCCAGAGCCG
  GACAATGATGCTCACCTACGCAGCTCCTCTCGCCCCTGCAGCCCCGTCCACCACCACGAG
  GGCCATGCCAAGCTGTCTAGCAGCCCCCCTCGTGCAAGCCCCGTGAGGATGGCACCGTCG
  TACGTGCTCAAGAAAGCCCAGGTATTGAGTGCTGGCAGCCGGAGGCGGAAGGAGCAGCGC
  TACCGCAGCGTCATCTCAGACATCTTTGACGGCTCCATTCTCAGCCTTGTGCAGTGTCTC
  ACCTGTGACCGGGTATCCACCACAGTGGAAACGTTCCAGGACTTATCACTGCCCATTCCT
  GGAAAGGAGGACCTGGCCAAGCTCCATTCAGCCATCTACCAGAATGTGCCGGCCAAGCCA
  GGCGCCTGTGGGGACAGCTATGCCGCCCAGGGCTGGCTGGCCTTCATTGTGGAGTACATC
  CGACGGTTTGTGGTATCCTGTACCCCCAGCTGGTTTTGGGGGCCTGTCGTCACCCTGGAA
  GACTGCCTTGCTGCCTTCTTTGCCGCTGATGAGTTAAAGGGTGACAACATGTACAGCTGT
  GAGCGGTGTAAGAAGCTGCGGAACGGAGTGAAGTACTGCAAAGTCCTGCGGTTGCCCGAG
  ATCCTGTGCATTCACCTAAAGCGCTTTCGGCACGAGGTGATGTACTCATTCAAGATCAAC
  AGCCACGTCTCCTTCCCCCTCGAGGGGCTCGACCTGCGCCCCTTCCTTGCCAAGGAGTGC
  ACATCCCAGATCACCACCTACGACCTCCTCTCGGTCATCTGCCACCACGGCACGGCAGGC
  AGTGGGCACTACATCGCCTACTGCCAGAACGTGATCAATGGGCAGTGGTACGAGTTTGAT
  GACCAGTACGTCACAGAAGTCCACGAGACGGTGGTGCAGAACGCCGAGGGCTACGTACTC
  TTCTACAGGAAGAGCAGCGAGGAGGCCATGCGGGAGCGACAGCAGGTGGTGTCCCTGGCC
  GCCATGCGGGAGCCCAGCCTGCTGCGGTTCTACGTGTCCCGCGAGTGGCTCAACAAGTTC
  AACACCTTCGCGGAGCCAGGCCCCATCACCAACCAGACCTTCCTCTGCTCCCACGGAGGC
  ATCCCGCCCCACAAATACCACTACATCGACGACCTGGTGGTCATCCTGCCCCAGAACGTC
  TGGGAGCACCTGTACAACAGATTCGGGGGTGGCCCCGCCGTGAACCACCTGTACGTGTGC
  TCCATCTGCCAGGTGGAGATCGAGGCACTGGCCAAGCGCAGGAGGATCGAGATCGACACC
  TTCATCAAGTTGAACAAGGCCTTCCAGGCCGAGGAGTCGCCGGGCGTCATCTACTGCATC
  AGCATGCAGTGGTTCCGGGAGTGGGAGGCGTTCGTCAAGGGGAAGGACAACGAGCCCCCC
  GGGCCCATTGACAACAGCAGGATTGCACAGGTCAAAGGAAGCGGCCATGTCCAGCTGAAG
  CAGGGAGCTGACTACGGGCAGATTTCGGAGGAGACCTGGACCTACCTGAACAGCCTGTAT
  GGAGGTGGCCCCGAGATTGCCATCCGCCAGAGTGTGGCGCAGCCGCTGGGCCCAGAGAAC
  CTGCACGGGGAGCAGAAGATCGAAGCCGAGACGCGGGCCGTG
  
• Restriction Sites: Please inquire
• ACCN: NM_001110303
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001110303.2, NP_001103773.2
• RefSeq Size: 4334 bp
• RefSeq ORF: 2745 bp
• Locus ID: 10868
• UniProt ID: Q9Y2K6
• Cytogenetics: 9q34.11
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes a ubiquitin specific processing protease that was first identified as a substrate of the VHL (von Hippel-Lindau disease) protein E3 ubiquitin ligase complex. In addition to being ubiquitinated by the VHL-E3 ligase complex, this enzyme deubiquitinates hypoxia-inducible factor (HIF)-1 alpha and thereby causes increased expression of HIF-1alpha targeted genes which play a role in angiogenesis, glucose metabolism, cell proliferation and metastasis. The enzyme encoded by this gene also regulates G-protein coupled receptor signaling by mediating the deubiquitination of beta-2 adrenergic receptor (ADRB2). This enzyme is a ubiquitously expressed thiolester hydrolase. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jan 2013]
  Transcript Variant: This variant (3) has a different 3' non-coding, terminal exon, compared to transcript variant 1. All three transcript variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments and orthologous data.