WFS1 (NM_001145853) Human Untagged Clone

CAT#: SC326504

WFS1 (untagged)-Human Wolfram syndrome 1 (wolframin) (WFS1), transcript variant 2, mRNA

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WFS1
• Synonyms: CTRCT41; WFRS; WFS; WFSL
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC326504 representing NM_001145853.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACTCCAACACTGCTCCGCTGGGCCCCTCCTGCCCACAGCCCCCGCCAGCACCGCAGCCCCAGGCG
  CGTTCCCGACTCAATGCCACAGCCTCGTTGGAGCAGGAGAGGAGCGAAAGGCCCCGAGCACCCGGACCC
  CAGGCTGGCCCTGGCCCTGGTGTTAGAGACGCAGCGGCCCCCGCTGAACCCCAGGCCCAGCATACCAGG
  AGCCGGGAAAGAGCAGACGGCACCGGGCCTACAAAGGGAGACATGGAAATCCCCTTTGAAGAAGTCCTG
  GAGAGGGCCAAGGCCGGGGACCCCAAGGCACAGACTGAGGTGGGGAAGCACTACCTGCAGTTGGCCGGC
  GACACGGATGAAGAACTCAACAGCTGCACCGCTGTGGACTGGCTGGTCCTCGCCGCGAAGCAGGGCCGT
  CGCGAGGCTGTGAAGCTGCTTCGCCGGTGCTTGGCGGACAGAAGAGGCATCACGTCCGAGAACGAACGG
  GAGGTGAGGCAGCTCTCCTCCGAGACCGACCTGGAGAGGGCCGTGCGCAAGGCAGCCCTGGTCATGTAC
  TGGAAGCTCAACCCCAAGAAGAAGAAGCAGGTGGCCGTGGCGGAGCTGCTGGAGAATGTCGGCCAGGTC
  AACGAGCACGATGGAGGGGCGCAGCCAGGCCCCGTGCCCAAGTCCCTGCAGAAGCAGAGGCGCATGCTG
  GAGCGCCTGGTCAGCAGCGAGTCCAAGAACTACATCGCGCTGGATGACTTTGTGGAGATCACTAAGAAG
  TACGCCAAGGGCGTCATCCCCAGCAGCCTGTTCCTGCAGGACGACGAAGATGATGACGAGCTGGCGGGG
  AAGAGCCCTGAGGACCTGCCACTGCGTCTGAAGGTGGTCAAGTACCCCCTGCACGCCATCATGGAGATC
  AAGGAGTACCTGATTGACATGGCCTCCAGGGCAGGCATGCACTGGCTGTCCACCATCATCCCCACGCAC
  CACATCAACGCGCTCATCTTCTTCTTCATCGTCAGCAACCTCACCATCGACTTCTTCGCCTTCTTCATC
  CCGCTGGTCATCTTCTACCTGTCCTTCATCTCCATGGTGATCTGCACCCTCAAGGTGTTCCAGGACAGC
  AAGGCCTGGGAGAACTTCCGCACCCTCACCGACCTGCTGCTGCGCTTCGAGCCCAACCTGGATGTGGAG
  CAGGCCGAGGTCAACTTCGGCTGGAACCACCTGGAGCCCTATGCCCATTTCCTGCTCTCTGTCTTCTTC
  GTCATCTTCTCCTTCCCCATCGCCAGCAAGGACTGCATCCCCTGCTCGGAGCTGGCTGTCATCACCGGC
  TTCTTTACCGTGACCAGCTACCTGAGCCTGAGCACCCATGCAGAGCCCTACACGCGCAGGGCCCTGGCC
  ACCGAGGTCACCGCCGGCCTGCTATCGCTGCTGCCCTCCATGCCCTTGAATTGGCCCTACCTGAAGGTC
  CTTGGCCAGACCTTCATCACCGTGCCTGTCGGCCACCTGGTCGTCCTCAACGTCAGCGTCCCGTGCCTG
  CTCTATGTCTACCTGCTCTATCTCTTCTTCCGCATGGCACAGCTGAGGAATTTCAAGGGCACCTACTGC
  TACCTTGTGCCCTACCTGGTGTGCTTCATGTGGTGTGAGCTCTCCGTGGTCATCCTGCTGGAGTCCACC
  GGCCTGGGGCTGCTCCGCGCCTCCATCGGCTACTTCCTCTTCCTCTTTGCCCTCCCCATCCTGGTGGCC
  GGCCTGGCCCTGGTGGGCGTGCTGCAGTTCGCCCGGTGGTTCACGTCTCTGGAGCTCACCAAGATCGCA
  GTCACCGTGGCGGTCTGTAGTGTGCCCCTGCTGTTGCGCTGGTGGACCAAGGCCAGCTTCTCTGTGGTG
  GGGATGGTGAAGTCCCTGACGCGGAGCTCCATGGTCAAGCTCATCCTGGTGTGGCTCACGGCCATCGTG
  CTGTTCTGCTGGTTCTATGTGTACCGCTCAGAGGGCATGAAGGTCTACAACTCCACACTGACCTGGCAG
  CAGTATGGTGCGCTGTGCGGGCCACGCGCCTGGAAGGAGACCAACATGGCGCGCACCCAGATCCTCTGC
  AGCCACCTGGAGGGCCACAGGGTCACGTGGACCGGCCGCTTCAAGTACGTCCGCGTGACTGACATCGAC
  AACAGCGCCGAGTCTGCCATCAACATGCTCCCGTTCTTCATCGGCGACTGGATGCGCTGCCTCTACGGC
  GAGGCCTACCCTGCCTGCAGCCCTGGCAACACCTCCACGGCCGAGGAGGAGCTCTGTCGCCTTAAGCTG
  CTGGCCAAGCACCCCTGCCACATCAAGAAGTTCGACCGCTACAAGTTTGAGATTACCGTGGGCATGCCA
  TTCAGCAGCGGCGCTGACGGCTCGCGCAGCCGCGAGGAGGACGACGTCACCAAGGACATCGTGCTGCGG
  GCCAGCAGCGAGTTCAAGAGCGTGCTGCTCAGCCTGCGCCAGGGCAGCCTCATCGAGTTCAGCACCATC
  CTGGAGGGCCGCCTGGGCAGCAAGTGGCCTGTCTTCGAGCTCAAGGCCATCAGCTGCCTCAACTGCATG
  GCCCAGCTCTCACCCACCAGGCGGCACGTGAAGATCGAGCACGACTGGCGCAGCACCGTGCATGGCGCC
  GTGAAGTTCGCCTTCGACTTCTTTTTCTTCCCATTCCTGTCGGCGGCCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001145853
• Insert Size: 2673 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001145853.1
• RefSeq Size: 3636 bp
• RefSeq ORF: 2673 bp
• Locus ID: 7466
• UniProt ID: O76024
• Cytogenetics: 4p16.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 100.3 kDa
• Gene Summary: This gene encodes a transmembrane protein, which is located primarily in the endoplasmic reticulum and ubiquitously expressed with highest levels in brain, pancreas, heart, and insulinoma beta-cell lines. Mutations in this gene are associated with Wolfram syndrome, also called DIDMOAD (Diabetes Insipidus, Diabetes Mellitus, Optic Atrophy, and Deafness), an autosomal recessive disorder. The disease affects the brain and central nervous system. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6), also known as DFNA14 or DFNA38. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2009]
  Transcript Variant: This variant (2) has an alternate splice site in the 5' UTR, resulting in a slightly shorter transcript, as compared to variant 1.