CD168 (HMMR) (NM_001142557) Human Untagged Clone

CAT#: SC326134

HMMR (untagged)-Human hyaluronan-mediated motility receptor (RHAMM) (HMMR), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD168
• Synonyms: CD168; IHABP; RHAMM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC326134 representing NM_001142557.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACCCTTCTGATTCGTGTTCTTCTACAGGAACGTGGTGCCCAGGACAGGCGGATCCAGGATCTGGAA
  ACTGAGTTGGAAAAGATGGAAGCAAGGCTAAATGCTGCACTAAGGGAAAAAACATCTCTCTCTGCAAAT
  AATGCTACACTGGAAAAACAACTTATTGAATTGACCAGGACTAATGAACTACTAAAATCTAAGTTTTCT
  GAAAATGGTAACCAGAAGAATTTGAGAATTCTAAGCTTGGAGTTGATGAAACTTAGAAACAAAAGAGAA
  ACAAAGATGAGGGGTATGATGGCTAAGCAAGAAGGCATGGAGATGAAGCTGCAGGTCACCCAAAGGAGT
  CTCGAAGAGTCTCAAGGGAAAATAGCCCAACTGGAGGGAAAACTTGTTTCAATAGAGAAAGAAAAGATT
  GATGAAAAATCTGAAACAGAAAAACTCTTGGAATACATCGAAGAAATTAGTTGTGCTTCAGATCAAGTG
  GAAAAATACAAGCTAGATATTGCCCAGTTAGAAGAAAATTTGAAAGAGAAGAATGATGAAATTTTAAGC
  CTTAAGCAGTCTCTTGAGGAGAATATTGTTATATTATCTAAACAAGTAGAAGATCTAAATGTGAAATGT
  CAGCTGCTTGAAAAAGAAAAAGAAGACCATGTCAACAGGAATAGAGAACACAACGAAAATCTAAATGCA
  GAGATGCAAAACTTAAAACAGAAGTTTATTCTTGAACAACAGGAACGTGAAAAGCTTCAACAAAAAGAA
  TTACAAATTGATTCACTTCTGCAACAAGAGAAAGAATTATCTTCGAGTCTTCATCAGAAGCTCTGTTCT
  TTTCAAGAGGAAATGGTTAAAGAGAAGAATCTGTTTGAGGAAGAATTAAAGCAAACACTGGATGAGCTT
  GATAAATTACAGCAAAAGGAGGAACAAGCTGAAAGGCTGGTCAAGCAATTGGAAGAGGAAGCAAAATCT
  AGAGCTGAAGAATTAAAACTCCTAGAAGAAAAGCTGAAAGGGAAGGAGGCTGAACTGGAGAAAAGTAGT
  GCTGCTCATACCCAGGCCACCCTGCTTTTGCAGGAAAAGTATGACAGTATGGTGCAAAGCCTTGAAGAT
  GTTACTGCTCAATTTGAAAGCTATAAAGCGTTAACAGCCAGTGAGATAGAAGATCTTAAGCTGGAGAAC
  TCATCATTACAGGAAAAAGCGGCCAAGGCTGGGAAAAATGCAGAGGATGTTCAGCATCAGATTTTGGCA
  ACTGAGAGCTCAAATCAAGAATATGTAAGGATGCTTCTAGATCTGCAGACCAAGTCAGCACTAAAGGAA
  ACAGAAATTAAAGAAATCACAGTTTCTTTTCTTCAAAAAATAACTGATTTGCAGAACCAACTCAAGCAA
  CAGGAGGAAGACTTTAGAAAACAGCTGGAAGATGAAGAAGGAAGAAAAGCTGAAAAAGAAAATACAACA
  GCAGAATTAACTGAAGAAATTAACAAGTGGCGTCTCCTCTATGAAGAACTATATAATAAAACAAAACCT
  TTTCAGCTACAACTAGATGCTTTTGAAGTAGAAAAACAGGCATTGTTGAATGAACATGGTGCAGCTCAG
  GAACAGCTAAATAAAATAAGAGATTCATATGCTAAATTATTGGGTCATCAGAATTTGAAACAAAAAATC
  AAGCATGTTGTGAAGTTGAAAGATGAAAATAGCCAACTCAAATCGGAAGTATCAAAACTCCGCTGTCAG
  CTTGCTAAAAAAAAACAAAGTGAGACAAAACTTCAAGAGGAATTGAATAAAGTTCTAGGTATCAAACAC
  TTTGATCCTTCAAAGGCTTTTCATCATGAAAGTAAAGAAAATTTTGCCCTGAAGACCCCATTAAAAGAA
  GGCAATACAAACTGTTACCGAGCTCCTATGGAGTGTCAAGAATCATGGAAGTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001142557
• Insert Size: 1917 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001142557.1
• RefSeq Size: 2917 bp
• RefSeq ORF: 1917 bp
• Locus ID: 3161
• UniProt ID: O75330
• Cytogenetics: 5q34
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: ECM-receptor interaction
• MW: 74.5 kDa
• Gene Summary: The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Dec 2008]
  Transcript Variant: This variant (4) is missing 3 consecutive internal coding exons compared to transcript variant 1. This results in translation initiation from an alternate AUG start site and a shorter isoform (d) with an unique N-terminus compared to isoform a.