YTHDC1 (NM_001031732) Human Untagged Clone

CAT#: SC324231

YTHDC1 (untagged)-Human YTH domain containing 1 (YTHDC1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: YTHDC1
• Synonyms: YT521; YT521-B
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001031732.2 CGCCGTCGCCATCTGTTTCCCTTCCCTCCCCCATACCTAGCCCGAGTCTGAGCCCTAACG
  AGAAGGCTGGGCCTAGGCCGCTGGATGCTGGAGTGAAAGGAAGGGAGAAAGGGAAAAAGC
  GGGAAGAGTCGAGAAGGCTGAGTGTTAAGAGGCCAAGTGCGACGCGCGTATCCGGGCAGA
  CGGACTGACGGACGGGCCCGTGCTTCTGCCGCGGCTGCGGCGCCCGCGCGAGTCGCGTCT
  AAGCGGCGGCGGCGGTGGCAGCGGCGGAAACCGAAGGGGAGCCATGGCGGCTGACAGTCG
  GGAGGAGAAAGATGGAGAACTTAATGTTCTGGATGATATTTTAACTGAAGTACCAGAACA
  AGATGATGAACTGTATAATCCAGAGAGTGAACAAGATAAAAATGAGAAAAAGGGATCAAA
  AAGAAAAAGTGATCGAATGGAATCTACTGATACCAAACGACAAAAGCCTTCTGTCCATTC
  AAGACAACTGGTTTCTAAGCCACTGAGCTCATCTGTTAGCAATAACAAAAGAATAGTTAG
  TACAAAAGGAAAGTCAGCCACAGAGTATAAAAATGAGGAATATCAAAGATCTGAAAGAAA
  CAAGCGTCTAGATGCTGATCGGAAAATTCGTCTATCAAGTAGTGCCTCCAGAGAACCTTA
  TAAGAATCAACCTGAAAAAACCTGTGTCCGGAAAAGGGATCCTGAAAGGAGGGCCAAATC
  TCCTACGCCAGATGGTTCTGAGAGAATTGGGCTTGAAGTGGATAGACGTGCAAGCAGATC
  CAGCCAGTCTTCTAAGGAAGAAGTGAACTCTGAAGAATATGGCTCTGACCATGAGACTGG
  CAGCAGTGGTTCTTCTGATGAGCAAGGGAACAACACTGAGAATGAGGAGGAAGGAGTGGA
  AGAAGATGTGGAGGAAGATGAAGAAGTAGAAGAAGATGCAGAAGAAGATGAAGAGGTGGA
  TGAAGATGGAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAAGAGGAGGAGGAGGAGGAGGA
  AGAAGAAGAATATGAACAGGATGAGAGAGACCAGAAAGAGGAGGGAAATGATTATGACAC
  TCGAAGTGAGGCCAGTGACTCTGGTTCTGAATCTGTTTCCTTCACAGATGGGTCTGTCAG
  ATCTGGTTCAGGCACAGATGGATCAGATGAGAAAAAGAAGGAAAGGAAGAGAGCTAGAGG
  CATATCTCCAATTGTTTTTGATAGAAGTGGAAGCTCTGCATCAGAGTCATATGCAGGTTC
  AGAAAAGAAGCATGAGAAATTATCATCTTCCGTTCGTGCTGTCCGAAAAGATCAAACCAG
  TAAACTCAAATATGTGCTTCAAGATGCAAGATTTTTCCTCATAAAGAGTAACAACCATGA
  GAATGTGTCTCTTGCCAAAGCGAAGGGTGTATGGTCCACGCTCCCTGTAAATGAGAAGAA
  ATTAAATCTTGCATTTAGATCTGCAAGGAGTGTTATCTTAATATTTTCTGTCAGAGAGAG
  TGGAAAATTTCAAGGGTTTGCAAGACTTTCTTCAGAATCACATCACGGAGGATCTCCTAT
  ACACTGGGTGCTTCCAGCAGGAATGAGTGCTAAAATGCTGGGAGGTGTCTTTAAAATTGA
  CTGGATTTGCAGGCGTGAATTACCCTTCACTAAGTCGGCTCATCTCACCAATCCTTGGAA
  TGAACATAAACCAGTAAAGATCGGACGTGATGGACAGGAAATTGAACTTGAATGTGGAAC
  CCAGCTTTGTCTTCTGTTTCCCCCCGATGAAAGTATTGACTTGTATCAGGTCATTCATAA
  AATGCGTCACAAGAGAAGAATGCATTCTCAGCCCCGATCACGAGGACGTCCATCCCGTCG
  AGAACCAGTCCGGGATGTGGGAAGGCGTCGACCAGAAGATTATGATATTCATAACAGCAG
  AAAGAAACCAAGGATTGACTATCCCCCTGAGTTTCACCAGAGACCAGGGTATTTAAAGGA
  TCCACGATACCAGGAAGTGGACAGACGATTTTCAGGAGTTCGCCGAGATGTGTTTTTAAA
  TGGGTCCTACAATGATTATGTGAGGGAATTTCATAACATGGGACCACCACCACCTTGGCA
  AGGAATGCCCCCTTACCCAGGAATGGAACAACCTCCACACCATCCTTACTATCAGCACCA
  TGCTCCACCTCCTCAAGCTCATCCCCCTTACTCAGGACATCATCCAGTACCACATGAAGC
  AAGATACAGAGATAAACGAGTACATGATTATGATATGAGGGTGGATGATTTCCTTCGTCG
  CACACAAGCTGTTGTCAGTGGCCGGAGAAGTAGACCCCGTGAAAGAGACCGGGAACGAGA
  GCGAGACCGCCCTAGAGATAACAGACGAGACAGAGAGCGAGATAGAGGACGTGATAGAGA
  AAGAGAAAGAGAGCGATTATGTGATCGAGACAGAGACCGAGGGGAGAGAGGTCGATATAG
  AAGATAATGGGCTTTTGGAAGCACTGATTGTTTAAAGATACAAAAAATCTTGTATTTTTT
  TTTTGTGTGTGTTTACAAGTAGTAAATTTATTTTCAGCTGTCTGCCTATGAAGTTCATTG
  TGTAGAAGGATTTATTATGACCCCCTTTGTTCCAAGCATGCAGTATCATAAGAACTGGAA
  AAACTCAAATCCGCCAAAAATCCACAGCTGACAGTTGAATTGACACTTTTATTGGGGCAG
  AATGGAACAGTCCAAGAATGTAGATACTGATTCTTTCTCCATAAATGTTCTTATAGTGTG
  TTCATCCTAGAGTTATTTTTTTGTTTGTTTTTTTCCTTTTTGGATCTTGATTGATAACTG
  CCATGATATTTTGCTTTGATGTGTTTCTACATGTAGTTGCACACGGTTCAGTAAAAATAA
  TGCTGCTATCGAGTATGCAAATATTGAAGTATGATGGTTTGACTGTATGGCAGTGTTGTA
  GCAGCCTCTTGTTTTTTTCCCCATTGCCTCTTTTTTTAAAAAACTTATAAAGTCACTTTT
  TATTTTTCCTCAGTCTTCAATGACGAGAGCAATATTAAGAAGACATTGCTATCTAATTTT
  TAATCTTTTTAAATGAAAAATTCCTATGTTCAGTAGCGTGGTTGATGCTATTGTTTAGCC
  TTCCCCTCCAAATTGTATACATTGGCTTGGAATGTTCACAACTTGCGTGCGTGGCAGCGG
  AAGACGATTCCCATATTCTACATTGCTACTGTTTTGTATAAAATAAATTGGTAAAGATTG
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001031732
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001031732.2, NP_001026902.1
• RefSeq Size: 6249 bp
• RefSeq ORF: 2184 bp
• Locus ID: 91746
• UniProt ID: Q96MU7
• Cytogenetics: 4q13.2
• Gene Summary: Regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs (PubMed:26318451, PubMed:26876937, PubMed:25242552, PubMed:28984244). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:26318451, PubMed:25242552). Acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10 (PubMed:26876937). Specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing (PubMed:26876937). In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing (PubMed:26876937). May also regulate alternative splice site selection (PubMed:20167602). Also involved in nuclear export of m6A-containing mRNAs via interaction with SRSF3: interaction with SRSF3 facilitates m6A-containing mRNA-binding to both SRSF3 and NXF1, promoting mRNA nuclear export (PubMed:28984244). Also recognizes and binds m6A on other RNA molecules (PubMed:27602518). Involved in random X inactivation mediated by Xist RNA: recognizes and binds m6A-containing Xist and promotes transcription repression activity of Xist (PubMed:27602518). Involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts, probably by binding m6A-containing MAT2A mRNAs (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.