SLFN14 (NM_001129820) Human Untagged Clone

CAT#: SC323234

SLFN14 (untagged)-Human schlafen family member 14 (SLFN14)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLFN14
• Synonyms: BDPLT20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC323234 representing NM_001129820.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAGAGTCTCAAGACTGATACTGAAATGCCGTATCCTGAGGTAATAGTAGATGTGGGCAGAGTGATT
  TTTGGAGAAGAAAACAGGAAGAAGATGACCAACAGCTGTTTGAAAAGATCTGAGAATTCTAGAATTATC
  CGGGCTATATGTGCACTGTTAAATTCTGGAGGTGGTGTGATCAAAGCAGAGATTGATGATAAAACCTAT
  AGTTACCAATGCCATGGGCTGGGACAGGATTTGGAAACTTCTTTTCAAAAGCTCCTTCCTTCAGGTTCA
  CAGAAATACCTTGACTACATGCAGCAGGGGCACAATCTCCTGATTTTTGTGAAGTCATGGAGCCCAGAT
  GTTTTCAGCCTTCCACTAAGGATTTGCAGCTTGCGCTCCAATTTGTATCGGAGAGATGTGACTTCTGCT
  ATCAACTTGAGTGCTAGCAGTGCCCTGGAGCTTCTCAGAGAGAAGGGGTTTAGAGCCCAAAGAGGAAGA
  CCAAGGGTGAAGAAGTTGCATCCTCAGCAGGTTCTCAATAGATGCATTCAGGAAGAGGAAGATATGAGG
  ATATTGGCCTCAGAATTTTTTAAAAAGGACAAACTCATGTATAAGGAGAAACTCAACTTTACTGAGTCA
  ACACATGTTGAATTTAAAAGGTTCACCACCAAAAAAGTCATACCTCGGATTAAGGAAATGCTGCCTCAT
  TATGTTTCTGCATTTGCCAACACTCAAGGGGGATATGTCCTCATTGGGGTGGATGATAAGAGCAAAGAA
  GTGGTTGGATGTAAGTGGGAAAAAGTGAATCCTGACTTACTAAAAAAAGAAATCGAAAACTGCATAGAA
  AAATTGCCTACATTCCACTTCTGCTGTGAGAAGCCAAAGGTAAATTTCACTACAAAAATCCTGAATGTG
  TACCAAAAAGATGTCCTGGATGGTTATGTCTGTGTGATTCAAGTGGAGCCCTTCTGTTGCGTGGTGTTT
  GCAGAGGCCCCAGATTCCTGGATCATGAAAGACAATTCTGTCACACGGCTGACAGCTGAGCAGTGGGTG
  GTCATGATGCTGGATACTCAGTCAGCTCCTCCCAGTTTGGTCACAGACTACAACTCTTGCCTGATTTCA
  TCAGCTTCATCTGCACGAAAAAGTCCCGGATATCCCATAAAAGTCCACAAATTTAAGGAGGCTCTGCAA
  CGACATTTGTTTCCAGTGACACAGGAAGAGGTACAATTTAAACCAGAATCCCTCTGTAAGAAGCTGTTC
  TCAGATCATAAAGAACTGGAGGGTTTAATGAAGACCCTGATACATCCTTGTTCTCAGGGGATTGTGATA
  TTTTCTAGAAGCTGGGCTGGTGATGTTGGCTTCAGGAAAGAACAGAATGTCCTGTGTGATGCTCTCCTG
  ATAGCAGTTAACAGCCCCGTGGTACTCTATACAATCTTAATAGACCCCAATTGGCCTGGAGGACTTGAA
  TATGCCCGAAACACAGCTCATCAGTTAAAGCAGAAACTGCAAACTGTTGGTGGTTACACAGGGAAAGTG
  TGCATCATTCCAAGGCTGATACACCTGAGCAGCACACAGAGTAGACCTGGTGAGATCCCCCTGCGTTAC
  CCCAGGTCCTACAGGCTTGCTGATGAGGAGGAAATGGAAGACTTGCTGCAGGCTCTGGTGGTGGTCTCC
  CTGTCCTCCAGATCTCTTCTGAGTGACCAGATGGGCTGTGAATTTTTCAACTTGCTCATAATGGAGCAG
  AGCCAGTTGCTTTCTGAGAGTCTTCAGAAGACACGTGAATTATTCATCTACTGCTTTCCAGGAGTCAGG
  AAGACAGCCCTAGCCATAAAGATCATGGAGAAAATTAAGGACTTGTTTCACTGCAAACCAAAAGAGATC
  CTCTATGTTTGTGAAAGCGACTCCCTAAAGGATTTTGTGACCCAACAAACCACCTGCCAAGCTGTGACC
  AGGAAAACTTTCATGCAAGGGGAGTTTCTAAAGATTAAACACATAGTGATGGATGAGACTGAGAATTTC
  TGCAGCAAATATGGCAATTGGTACATGAAGGCTAAGAACATCACCCATCCAAAGGCGAAGGGGACTGGA
  AGTGAAAACCTTCACCATGGGATTCTCTGGCTTTTTCTTGACCCTTTTCAAATCCATCACGCAGATGTC
  AATGGCCTTCCCCCTCCATCTGCTCAGTTTCCTCGAAAAACAATCACCAGTGGGATCCACTGTGCTCTG
  GAAATAGCGAAGGTTATGAAAGAAGAAATGAAGAGGATCAAAGAAAATCCTCCCTCCAACATGTCTCCA
  GACACATTAGCATTGTTCAGCGAGACTGCCTATGAGGAAGCAACGTGTGCCCAGGCTCTGCCTGGGGTG
  TGTGAGACAAAGACTAATCTGACAACAGAACAAATAGCTAACTATGTGGCAAGAAAATGTCACAGCCTG
  TTCCAGTGTGGCTATCTGCCCAAAGATATAGCAATTCTGTGCAGGAGAGGGGAGGACAGAGGACGCTAT
  AGGCTTGCACTACTCAAAGCAATGGAATTAATTGAGACCCACAGACCATCAGAGGTTGTGTTTAGCCCG
  GCCACCGGTGTTTGGGGAAGTCACATTGTTTTAGACAGTATTCAGCAATTTTCAGGCCTGGAGAGGACT
  GTCGTGTTTGGGCTTAGTCCAGAATGTGACCAGTCAGAGGAATTTCATAAGCTCTGCTTTGCTTCAAGA
  GCCATTAAACACCTCTACCTGCTTTATGAAAAGAGGGCAGCCTACTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001129820
• Insert Size: 2739 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001129820.1
• RefSeq Size: 2882 bp
• RefSeq ORF: 2739 bp
• Locus ID: 342618
• UniProt ID: P0C7P3
• Cytogenetics: 17q12
• MW: 103.9 kDa
• Gene Summary: The protein encoded by this gene plays an important role in platelet formation and function. Defects in this gene are a cause of thrombocytopenia with excessive bleeding. [provided by RefSeq, Jul 2016]