SLFN14 (NM_001129820) Human Tagged ORF Clone

CAT#: RG226257

SLFN14 (tGFP-tagged) - Human schlafen family member 14 (SLFN14)

Product Images

Circular map for RG226257

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: SLFN14
• Synonyms: BDPLT20
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG226257 representing NM_001129820
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGTCTCAAGACTGATACTGAAATGCCGTATCCTGAGGTAATAGTAGATGTGGGCAGAGTGATTT
  TTGGAGAAGAAAACAGGAAGAAGATGACCAACAGCTGTTTGAAAAGATCTGAGAATTCTAGAATTATCCG
  GGCTATATGTGCACTGTTAAATTCTGGAGGTGGTGTGATCAAAGCAGAGATTGATGATAAAACCTATAGT
  TACCAATGCCATGGGCTGGGACAGGATTTGGAAACTTCTTTTCAAAAGCTCCTTCCTTCAGGTTCACAGA
  AATACCTTGACTACATGCAGCAGGGGCACAATCTCCTGATTTTTGTGAAGTCATGGAGCCCAGATGTTTT
  CAGCCTTCCACTAAGGATTTGCAGCTTGCGCTCCAATTTGTATCGGAGAGATGTGACTTCTGCTATCAAC
  TTGAGTGCTAGCAGTGCCCTGGAGCTTCTCAGAGAGAAGGGGTTTAGAGCCCAAAGAGGAAGACCAAGGG
  TGAAGAAGTTGCATCCTCAGCAGGTTCTCAATAGATGCATTCAGGAAGAGGAAGATATGAGGATATTGGC
  CTCAGAATTTTTTAAAAAGGACAAACTCATGTATAAGGAGAAACTCAACTTTACTGAGTCAACACATGTT
  GAATTTAAAAGGTTCACCACCAAAAAAGTCATACCTCGGATTAAGGAAATGCTGCCTCATTATGTTTCTG
  CATTTGCCAACACTCAAGGGGGATATGTCCTCATTGGGGTGGATGATAAGAGCAAAGAAGTGGTTGGATG
  TAAGTGGGAAAAAGTGAATCCTGACTTACTAAAAAAAGAAATCGAAAACTGCATAGAAAAATTGCCTACA
  TTCCACTTCTGCTGTGAGAAGCCAAAGGTAAATTTCACTACAAAAATCCTGAATGTGTACCAAAAAGATG
  TCCTGGATGGTTATGTCTGTGTGATTCAAGTGGAGCCCTTCTGTTGCGTGGTGTTTGCAGAGGCCCCAGA
  TTCCTGGATCATGAAAGACAATTCTGTCACACGGCTGACAGCTGAGCAGTGGGTGGTCATGATGCTGGAT
  ACTCAGTCAGCTCCTCCCAGTTTGGTCACAGACTACAACTCTTGCCTGATTTCATCAGCTTCATCTGCAC
  GAAAAAGTCCCGGATATCCCATAAAAGTCCACAAATTTAAGGAGGCTCTGCAACGACATTTGTTTCCAGT
  GACACAGGAAGAGGTACAATTTAAACCAGAATCCCTCTGTAAGAAGCTGTTCTCAGATCATAAAGAACTG
  GAGGGTTTAATGAAGACCCTGATACATCCTTGTTCTCAGGGGATTGTGATATTTTCTAGAAGCTGGGCTG
  GTGATGTTGGCTTCAGGAAAGAACAGAATGTCCTGTGTGATGCTCTCCTGATAGCAGTTAACAGCCCCGT
  GGTACTCTATACAATCTTAATAGACCCCAATTGGCCTGGAGGACTTGAATATGCCCGAAACACAGCTCAT
  CAGTTAAAGCAGAAACTGCAAACTGTTGGTGGTTACACAGGGAAAGTGTGCATCATTCCAAGGCTGATAC
  ACCTGAGCAGCACACAGAGTAGACCTGGTGAGATCCCCCTGCGTTACCCCAGGTCCTACAGGCTTGCTGA
  TGAGGAGGAAATGGAAGACTTGCTGCAGGCTCTGGTGGTGGTCTCCCTGTCCTCCAGATCTCTTCTGAGT
  GACCAGATGGGCTGTGAATTTTTCAACTTGCTCATAATGGAGCAGAGCCAGTTGCTTTCTGAGAGTCTTC
  AGAAGACACGTGAATTATTCATCTACTGCTTTCCAGGAGTCAGGAAGACAGCCCTAGCCATAAAGATCAT
  GGAGAAAATTAAGGACTTGTTTCACTGCAAACCAAAAGAGATCCTCTATGTTTGTGAAAGCGACTCCCTA
  AAGGATTTTGTGACCCAACAAACCACCTGCCAAGCTGTGACCAGGAAAACTTTCATGCAAGGGGAGTTTC
  TAAAGATTAAACACATAGTGATGGATGAGACTGAGAATTTCTGCAGCAAATATGGCAATTGGTACATGAA
  GGCTAAGAACATCACCCATCCAAAGGCGAAGGGGACTGGAAGTGAAAACCTTCACCATGGGATTCTCTGG
  CTTTTTCTTGACCCTTTTCAAATCCATCACGCAGATGTCAATGGCCTTCCCCCTCCATCTGCTCAGTTTC
  CTCGAAAAACAATCACCAGTGGGATCCACTGTGCTCTGGAAATAGCGAAGGTTATGAAAGAAGAAATGAA
  GAGGATCAAAGAAAATCCTCCCTCCAACATGTCTCCAGACACATTAGCATTGTTCAGCGAGACTGCCTAT
  GAGGAAGCAACGTGTGCCCAGGCTCTGCCTGGGGTGTGTGAGACAAAGACTAATCTGACAACAGAACAAA
  TAGCTAACTATGTGGCAAGAAAATGTCACAGCCTGTTCCAGTGTGGCTATCTGCCCAAAGATATAGCAAT
  TCTGTGCAGGAGAGGGGAGGACAGAGGACGCTATAGGCTTGCACTACTCAAAGCAATGGAATTAATTGAG
  ACCCACAGACCATCAGAGGTTGTGTTTAGCCCGGCCACCGGTGTTTGGGGAAGTCACATTGTTTTAGACA
  GTATTCAGCAATTTTCAGGCCTGGAGAGGACTGTCGTGTTTGGGCTTAGTCCAGAATGTGACCAGTCAGA
  GGAATTTCATAAGCTCTGCTTTGCTTCAAGAGCCATTAAACACCTCTACCTGCTTTATGAAAAGAGGGCA
  GCCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG226257 representing NM_001129820
  Red=Cloning site Green=Tags(s)
  
  MESLKTDTEMPYPEVIVDVGRVIFGEENRKKMTNSCLKRSENSRIIRAICALLNSGGGVIKAEIDDKTYS
  YQCHGLGQDLETSFQKLLPSGSQKYLDYMQQGHNLLIFVKSWSPDVFSLPLRICSLRSNLYRRDVTSAIN
  LSASSALELLREKGFRAQRGRPRVKKLHPQQVLNRCIQEEEDMRILASEFFKKDKLMYKEKLNFTESTHV
  EFKRFTTKKVIPRIKEMLPHYVSAFANTQGGYVLIGVDDKSKEVVGCKWEKVNPDLLKKEIENCIEKLPT
  FHFCCEKPKVNFTTKILNVYQKDVLDGYVCVIQVEPFCCVVFAEAPDSWIMKDNSVTRLTAEQWVVMMLD
  TQSAPPSLVTDYNSCLISSASSARKSPGYPIKVHKFKEALQRHLFPVTQEEVQFKPESLCKKLFSDHKEL
  EGLMKTLIHPCSQGIVIFSRSWAGDVGFRKEQNVLCDALLIAVNSPVVLYTILIDPNWPGGLEYARNTAH
  QLKQKLQTVGGYTGKVCIIPRLIHLSSTQSRPGEIPLRYPRSYRLADEEEMEDLLQALVVVSLSSRSLLS
  DQMGCEFFNLLIMEQSQLLSESLQKTRELFIYCFPGVRKTALAIKIMEKIKDLFHCKPKEILYVCESDSL
  KDFVTQQTTCQAVTRKTFMQGEFLKIKHIVMDETENFCSKYGNWYMKAKNITHPKAKGTGSENLHHGILW
  LFLDPFQIHHADVNGLPPPSAQFPRKTITSGIHCALEIAKVMKEEMKRIKENPPSNMSPDTLALFSETAY
  EEATCAQALPGVCETKTNLTTEQIANYVARKCHSLFQCGYLPKDIAILCRRGEDRGRYRLALLKAMELIE
  THRPSEVVFSPATGVWGSHIVLDSIQQFSGLERTVVFGLSPECDQSEEFHKLCFASRAIKHLYLLYEKRA
  AY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001129820
• ORF Size: 2736 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001129820.2
• RefSeq Size: 2882 bp
• RefSeq ORF: 2739 bp
• Locus ID: 342618
• UniProt ID: P0C7P3
• Cytogenetics: 17q12
• Gene Summary: The protein encoded by this gene plays an important role in platelet formation and function. Defects in this gene are a cause of thrombocytopenia with excessive bleeding. [provided by RefSeq, Jul 2016]