PCCA (NM_001127692) Human Untagged Clone

CAT#: SC323177

PCCA (untagged)-Human propionyl CoA carboxylase, alpha polypeptide (PCCA), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCCA
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001127692, the custom clone sequence may differ by one or more nucleotides
  ATGGCGGGGTTCTGGGTCGGGACAGCACCGCTGGTCGCTGCCGGACGGCGTGGGCGGTGG
  CCGCCGCAGCAGCTGATGCTGAGCGCGGCGCTGCGGACCCTGAAGACTTTTGATAAAATT
  CTTGTTGCTAATAGAGGAGAAATTGCATGTCGGGTTATTAGAACTTGCAAGAAGATGGGC
  ATTAAGACAGTTGCCATCCACAGTGATGTTGATGCTAGTTCTGTTCATGTGAAAATGGCG
  GATGAGGCTGTCTGTGTTGGCCCAGCTCCCACCAGTAAAAGCTACCTCAACATGGATGCC
  ATCATGGAAGCCATTAAGAAAACCAGGGCCCAAGCTGTACATCCAGGTTATGGATTCCTT
  TCAGAAAACAAAGAATTTGCCAGATGTTTGGCAGCAGAAGATGTCGTTTTCATTGGACCT
  GACACACATGCTATTCAAGCCATGGGCGACAAGATTGAAAGCAAATTATTAGCTAAGAAA
  GCAGAGGTTAATACAATCCCTGGCTTTGATGGAGTAGTCAAGGATGCAGAAGAAGCTGTC
  AGAATTGCAAGGGAAATTGGCTACCCTGTCATGATCAAGGCCTCAGCAGGTGGTGGTGGG
  AAAGGCATGCGCATTGCTTGGGATGATGAAGAGACCAGGGATGGTTTTAGATTGTCATCT
  CAAGAAGCTGCTTCTAGTTTTGGCGATGATAGACTACTAATAGAAAAATTTATTGATAAT
  CCTCGTCATATAGAAATCCAGGTTCTAGGTGATAAACATGGGAATGCTTTATGGCTTAAT
  GAAAGAGAGTGCTCAATTCAGAGAAGAAATCAGAAGGTGGTGGAGGAAGCACCAAGCATT
  TTTTTGGATGCGGAGACTCGAAGAGCGATGGGAGAACAAGCTGTAGCTCTTGCCAGAGCA
  GTAAAATATTCCTCTGCTGGGACCGTGGAGTTCCTTGTGGACTCTAAGAAGAATTTTTAT
  TTCTTGGAAATGAATACAAGACTCCAGGTTGAGCATCCTGTCACAGAATGCATTACTGGC
  CTGGACCTAGTCCAGGAAATGATCCGTGTTGCTAAGGGCTACCCTCTCAGGCACAAACAA
  GCTGATATTCGCATCAACGGCTGGGCAGTTGAATGTCGGGTTTATGCTGAGGACCCCTAC
  AAGTCTTTTGGTTTACCATCTATTGGGAGATTGTCTCAGTACCAAGAACCGTTACATCTA
  CCTGGTGTCCGAGTGGACAGTGGCATCCAACCAGGAAGTGATATTAGCATTTATTATGAT
  CCTATGATTTCAAAACTAATCACATATGGCTCTGATAGAACTGAGGCACTGAAGAGAATG
  GCAGATGCACTGGATAACTATGTTATTCGAGGTGTTACACATAATATTGCATTACTTCGA
  GAGGTGATAATCAACTCACGCTTTGTAAAAGGAGACATCAGCACTAAATTTCTCTCCGAT
  GTGTATCCTGATGGCTTCAAAGGACACATGCTAACCAAGAGTGAGAAGAACCAGTTATTG
  GCAATAGCATCATCATTGTTTGTGGCATTCCAGTTAAGAGCACAACATTTTCAAGAAAAT
  TCAAGAATGCCTGTTATTAAACCAGACATAGCCAACTGGGAGCTCTCAGTAAAATTGCAT
  GATAAAGTTCATACCGTAGTAGCATCAAACAATGGGTCAGTGTTCTCGGTGGAAGTTGAT
  GGGTCGAAACTAAATGTGACCAGCACGTGGAACCTGGCTTCGCCCTTATTGTCTGTCAGC
  GTTGATGGCACTCAGAGGACTGTCCAGTGTCTTTCTCGAGAAGCAGGTGGAAACATGAGC
  ATTCAGTTTCTTGGTACAGTGTACAAGGTGAATATCTTAACCAGACTTGCCGCAGAATTG
  AACAAATTTATGCTGGAAAAAGTGACTGAGGACACAAGCAGTGTTCTGCGTTCCCCGATG
  CCCGGAGTGGTGGTGGCCGTCTCTGTCAAGCCTGGAGACGCGGTAGCAGAAGGTCAAGAA
  ATTTGTGTGATTGAAGCCATGAAAATGCAGAATAGTATGACAGCTGGGAAAACTGGCACG
  GTGAAATCTGTGCACTGTCAAGCTGGAGACACAGTTGGAGAAGGGGATCTGCTCGTGGAG
  CTGGAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001127692
• Insert Size: 2499 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001127692.1, NP_001121164.1
• RefSeq Size: 2499 bp
• RefSeq ORF: 2499 bp
• Locus ID: 5095
• UniProt ID: P05165
• Cytogenetics: 13q32.3
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: The protein encoded by this gene is the alpha subunit of the heterodimeric mitochondrial enzyme Propionyl-CoA carboxylase. PCCA encodes the biotin-binding region of this enzyme. Mutations in either PCCA or PCCB (encoding the beta subunit) lead to an enzyme deficiency resulting in propionic acidemia. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to NM_000282.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.