FBXL20 (NM_032875) Human Untagged Clone

CAT#: SC320289

FBXL20 (untagged)-Human F-box and leucine-rich repeat protein 20 (FBXL20), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FBXL20
• Synonyms: Fbl2; Fbl20
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032875.1 GAGAGCCGAGCTAGCGACGAGCAGTCGTTGCGGCCGCCGGCGCCGCGGGAGGTGGTGGAG
  GCCTAGCCGGAGCCGAGAGGTCTCTTGTTCCCGTCCCACGGTCCCGGCGTCACCCCTCCG
  GCGCCCAGTCCCCGTCCCGGAACTCCCGGGCCTGTCCTGGGCCCCCGGTCTGTGCACTCC
  GCTCGCCGCAGCGCCCGGCCCGGGCCGCACCCGCCGGCCCCATGAGGAGGGACGTGAACG
  GAGTGACCAAGAGCAGGTTTGAGATGTTCTCAAATAGTGATGAAGCTGTAATCAATAAAA
  AACTTCCCAAAGAACTCCTGTTACGGATATTTTCTTTTCTAGATGTTGTTACCCTGTGCC
  GCTGTGCTCAGGTCTCCAGGGCCTGGAATGTTCTGGCTCTGGATGGCAGTAACTGGCAGC
  GAATTGACCTATTTGATTTCCAGAGGGATATTGAGGGCCGAGTAGTGGAGAATATTTCAA
  AACGATGTGGGGGCTTTTTACGAAAGTTAAGTCTTCGTGGATGTCTTGGAGTGGGAGACA
  ATGCATTAAGAACCTTTGCACAAAACTGCAGGAACATTGAAGTACTGAATCTAAATGGGT
  GTACAAAGACAACAGACGCTACATGTACTAGCCTTAGCAAGTTCTGTTCCAAACTCAGGC
  ACCTTGACTTGGCTTCCTGTACATCAATAACAAACATGTCTCTAAAAGCTCTGAGTGAGG
  GATGTCCACTGTTGGAGCAGTTGAACATTTCCTGGTGTGACCAAGTAACCAAGGATGGCA
  TTCAAGCACTAGTGAGGGGCTGTGGGGGTCTCAAGGCCTTATTCTTAAAAGGCTGCACGC
  AGCTAGAAGATGAAGCTCTCAAGTACATAGGTGCACACTGCCCTGAACTGGTGACTTTGA
  ACTTGCAGACTTGCTTGCAAATCACAGATGAAGGTCTCATTACTATATGCAGAGGGTGCC
  ATAAGTTACAATCCCTTTGTGCCTCTGGCTGCTCCAACATCACAGATGCCATCCTGAATG
  CTCTAGGTCAGAACTGCCCACGGCTTAGAATATTGGAAGTGGCAAGATGTTCTCAATTAA
  CAGATGTGGGCTTTACCACTCTAGCCAGGAATTGCCATGAACTTGAAAAGATGGACCTGG
  AAGAGTGTGTTCAGATAACAGATAGCACATTAATCCAACTTTCTATACACTGTCCTCGAC
  TTCAAGTATTGAGTCTGTCTCACTGTGAGCTGATCACAGATGATGGAATTCGTCACCTGG
  GGAATGGGGCCTGCGCCCATGACCAGCTGGAGGTGATTGAGCTGGACAACTGCCCACTAA
  TCACAGATGCATCCCTGGAGCACTTGAAGAGCTGTCATAGCCTTGAGCGGATAGAACTCT
  ATGACTGCCAGCAAATCACACGGGCTGGAATCAAGAGACTCAGGACCCATTTACCCAATA
  TTAAAGTCCACGCCTACTTCGCACCTGTCACTCCACCCCCATCAGTAGGGGGCAGCAGAC
  AGCGCTTCTGCAGATGCTGCATCATCCTATGACAATGGAGGTGGTCAACCTTGGCGAACT
  GAGTATTTAATGACACTTCTAGAGCTACCGTGGAGTCTCTCCAGTGGAAGCAACCCCAGT
  GTTCTGAGCAAGGGTTACAAAGTGAGGGAGGGCAGTGTCCAGATCCCCAGAGCCACACAT
  ACATACACATACACACCCTTACCCCCATCCACTCTAGCTTTGTGACCATGGGACTGAAGT
  TTGTGATGGCTTTTTTATCAAGTAGATTGGTAAAATTTAACCATTCCTGTTGAGGTGCCC
  ATAAGAAAATCATAGGCCAAGATAGGGAGGGGCATTCCAGCAAACCCCGTGTTAATGCTA
  CTGTGGTTTTTAAATTTTTGTCTAGGGGTTTCTTTGGGGATTTTAGAACAGCATCTGCTG
  TCCTCCGGGGTCAAGAAAAGCATGGAAAGACAATATATGATGTACCCAGGGACCAGAAAG
  AAAATTTCTTTGCATCTTAGAAATGGTAGACATTCATTGTGACTAAAGAGCTTCTATGCT
  TCCTTGTTTCCATGCCAACATGCTGAGCATGCTCACAAAGAAGGCTCGTCCATTCCTCCT
  GTGTTTTAGTATTTGGCCCAGAGGTTTCCTAAATGGTTGCCTTGAAATCACTGTGGTCCA
  AATGTAATTCTTACACACTCAAATTATCACTGTCTGTAGCACACTTGTGCACCTGTCTTA
  CATTCTCTGTTGCTCCCCCCCACACTCTTGCTCAGTCTGTCACCTGTTCAGTCTGCTTAC
  TCACTCAATTGTTACCCTTTTGCTGTTGTCGTGTTTACAGTTTGCATTTTGAATGATTAG
  TTGGGATTACCAAACATTTTTTAAAAAGATATTATCAATAAATATTTTTTTAATTCTAAA
  TTTTAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_032875
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032875.1, NP_116264.2
• RefSeq Size: 2423 bp
• RefSeq ORF: 1311 bp
• Locus ID: 84961
• UniProt ID: Q96IG2
• Cytogenetics: 17q12
• Domains: LRR, F-box, LRR_CC
• Protein Families: Druggable Genome
• Gene Summary: Members of the F-box protein family, such as FBXL20, are characterized by an approximately 40-amino acid F-box motif. SCF complexes, formed by SKP1 (MIM 601434), cullin (see CUL1; MIM 603134), and F-box proteins, act as protein-ubiquitin ligases. F-box proteins interact with SKP1 through the F box, and they interact with ubiquitination targets through other protein interaction domains (Jin et al., 2004 [PubMed 15520277]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (1) encodes isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.