USP53 (NM_019050) Human Untagged Clone

CAT#: SC318481

USP53 (untagged)-Human ubiquitin specific peptidase 53 (USP53)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP53
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318481 representing NM_019050.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCATGGGTAAAATTCTTACGGAAACCTGGTGGCAATCTTGGAAAAGTTTATCAGCCTGGAAGTATG
  CTATCACTAGCCCCTACCAAAGGCTTGTTAAATGAACCAGGACAAAACAGCTGCTTTCTTAATAGCGCT
  GTACAGGTTTTATGGCAATTGGATATATTCCGACGAAGCTTGCGGGTTTTGACTGGACATGTTTGTCAG
  GGAGATGCCTGTATATTTTGTGCATTGAAGACGATATTTGCACAGTTCCAACACAGTCGAGAAAAAGCA
  CTTCCCTCAGATAACATAAGGCATGCTCTTGCAGAAAGTTTCAAAGATGAGCAGCGATTTCAACTTGGC
  CTTATGGATGATGCTGCGGAGTGCTTTGAAAATATGTTGGAGAGGATTCATTTTCACATAGTGCCAAGC
  AGAGATGCAGACATGTGTACCTCTAAATCTTGTATCACTCACCAGAAGTTTGCTATGACTCTGTATGAA
  CAGTGTGTGTGTCGTAGCTGTGGAGCATCGTCAGATCCTCTACCTTTTACAGAATTTGTGCGGTACATT
  TCTACAACAGCCTTATGCAATGAGGTTGAAAGAATGTTGGAAAGGCATGAACGCTTTAAACCTGAAATG
  TTTGCAGAATTGCTACAAGCAGCAAATACAACAGATGACTATAGGAAATGTCCTAGTAACTGTGGCCAA
  AAAATAAAAATTCGCCGTGTTTTAATGAATTGCCCAGAGATTGTTACAATTGGTTTAGTCTGGGACTCC
  GAGCATTCTGACTTGACCGAAGCTGTTGTTCGGAATCTAGCAACACATCTTTATCTTCCTGGGCTTTTT
  TATAGAGTTACTGATGAAAATGCCAAAAATAGTGAACTTAACCTTGTTGGTATGATCTGCTACACCAGC
  CAACATTATTGTGCCTTTGCATTTCACACCAAAAGTTCCAAATGGGTATTTTTTGATGATGCAAATGTG
  AAAGAGATTGGAACTAGATGGAAAGATGTTGTCTCCAAATGCATTCGATGCCACTTTCAGCCACTACTT
  TTGTTTTATGCAAACCCAGATGGCACAGCAGTTTCTACTGAGGATGCACTCAGGCAGGTCATCAGCTGG
  TCACATTACAAATCTGTTGCAGAAAATATGGGATGTGAAAAGCCTGTAATTCATAAGTCAGATAATTTA
  AAAGAAAATGGATTTGGTGATCAGGCAAAGCAGAGAGAAAATCAGAAATTTCCAACTGATAATATTTCA
  TCATCTAATCGGAGCCACAGTCACACAGGTGTAGGGAAAGGACCAGCTAAGTTAAGTCACATTGATCAA
  AGGGAAAAGATAAAAGACATTTCCAGAGAATGTGCTCTGAAAGCTATTGAACAGAAAAACTTACTTTCT
  TCACAAAGGAAAGATTTAGAGAAGGGACAAAGAAAAGATTTAGGACGACATAGAGATTTGGTTGATGAA
  GACCTTTCACATTTCCAATCTGGATCACCTCCTGCCCCAAATGGTTTTAAACAACATGGGAATCCACAT
  CTATATCATAGTCAAGGAAAAGGATCATATAAACATGACCGAGTTGTACCTCAGAGTCGAGCTTCTGCA
  CAAATAATAAGTTCAAGTAAATCCCAGATTCTTGCTCCAGGAGAGAAAATAACTGGCAAAGTTAAGAGT
  GACAATGGCACTGGATATGACACAGACAGCAGCCAAGATTCTAGGGATAGAGGAAACAGCTGTGATAGC
  AGCAGTAAAAGCCGGAACCGAGGTTGGAAACCTATGAGAGAAACATTAAATGTTGATAGTATTTTTAGT
  GAAAGTGAAAAAAGACAGCATAGTCCAAGACATAAACCAAATATCAGTAATAAGCCTAAATCTAGCAAG
  GATCCGAGTTTTAGTAATTGGCCAAAAGAGAATCCAAAGCAAAAAGGTTTAATGACCATATATGAAGAT
  GAAATGAAGCAGGAAATAGGAAGCAGAAGTTCCCTTGAATCTAATGGAAAAGGAGCAGAGAAAAATAAA
  GGCCTTGTAGAGGGTAAAGTGCATGGTGATAATTGGCAGATGCAAAGGACTGAGTCTGGATATGAAAGC
  AGTGATCACATCAGTAATGGTTCTACTAATTTGGACTCACCTGTTATCGATGGAAATGGTACAGTAATG
  GATATCAGTGGTGTTAAAGAAACAGTATGCTTCAGTGACCAGATTACGACAAGCAACCTAAATAAAGAA
  CGTGGGGACTGTACCTCCCTTCAGAGCCAACATCACTTAGAAGGCTTTAGAAAAGAACTCAGGAATTTG
  GAAGCAGGCTATAAATCTCATGAATTCCACCCAGAATCACATTTACAAATAAAAAATCATTTGATAAAA
  AGATCACATGTACATGAAGACAATGGAAAGTTATTTCCTTCATCCAGTCTACAAATACCCAAGGACCAT
  AATGCAAGAGAACATATCCACCAGTCAGATGAACAGAAACTTGAAAAACCGAATGAATGCAAATTTTCT
  GAGTGGCTTAATATAGAAAATTCTGAGAGAACAGGTTTGCCTTTTCACGTTGATAACTCTGCTTCTGGG
  AAGAGAGTGAACAGTAATGAACCATCTTCATTATGGTCTTCACACCTAAGAACTGTTGGGTTAAAGCCA
  GAAACTGCTCCTCTCATCCAGCAACAAAATATCATGGATCAATGTTACTTTGAGAACTCTCTATCCACA
  GAATGTATAATTCGGTCAGCCAGCAGATCTGATGGGTGTCAGATGCCAAAACTTTTTTGCCAGAATCTA
  CCACCCCCTTTGCCACCAAAGAAATATGCTATAACCAGTGTGCCACAGTCAGAGAAAAGCGAATCTACA
  CCTGATGTCAAACTTACAGAGGTGTTTAAAGCTACCTCTCATCTTCCGAAGCACAGTTTAAGTACAGCT
  TCAGAACCAAGTTTAGAAGTGAGTACACATATGAATGATGAAAGACATAAAGAAACATTTCAAGTGAGA
  GAATGTTTTGGCAACACACCAAACTGTCCATCCAGCTCCTCAACTAACGATTTTCAGGCAAACTCAGGT
  GCCATTGATGCATTTTGCCAACCAGAACTAGACTCTATTTCTACCTGTCCAAATGAGACAGTTTCATTA
  ACTACCTATTTTTCAGTTGATAGCTGCATGACGGATACATATAGATTGAAATACCATCAGAGGCCCAAG
  CTCTCTTTTCCAGAGAGCAGTGGCTTTTGTAATAATTCACTATCTTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_019050
• Insert Size: 3222 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_019050.2
• RefSeq Size: 6612 bp
• RefSeq ORF: 3222 bp
• Locus ID: 54532
• UniProt ID: Q70EK8
• Cytogenetics: 4q26
• MW: 120.8 kDa
• Gene Summary: Tight junction-associated protein that is involved in the survival of auditory hair cells and hearing. Maybe by modulating the barrier properties and mechanical stability of tight junctions (By similarity). Has no peptidase activity (PubMed:14715245).[UniProtKB/Swiss-Prot Function]