HERC6 (NM_017912) Human Untagged Clone

CAT#: SC318447

HERC6 (untagged)-Human hect domain and RLD 6 (HERC6), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HERC6
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_017912 edited
  GGAGCCTGTCGCAGCGGGACCGACGGAATCCGGAGCAGGCGACAGGGCGCAGAAGCGGGA
  TGTACTTCTGTTGGGGCGCCGACTCCAGGGAGCTGCAGCGCCGGAGGACGGCGGGCAGCC
  CCGGGGCTGAGCTACTGCAGGCGGCCAGCGGGGAGCGCCACTCTCTGCTGCTGCTGACCA
  ACCACAGGGTCCTCTCGTGCGGAGACAACAGCAGGGGTCAGCTGGGCCGCAGGGGCGCGC
  AGCGCGGGGAGCTGCCAGAACCAATTCAGGCATTGGAAACCCTAATTGTTGATCTCGTGA
  GCTGCGGGAAGGAGCACTCCCTGGCTGTGTGCCACAAAGGAAGGGTCTTCGCATGGGGAG
  CTGGTTCTGAAGGGCAGCTGGGGATTGGAGAATTCAAGGAAATAAGTTTCACACCTAAGA
  AAATAATGACTCTGAATGATATAAAAATAATACAAGTTTCCTGTGGACACTACCACTCCC
  TGGCATTATCAAAAGATAGCCAAGTGTTTTCGTGGGGAAAGAACAGCCATGGGCAGCTGG
  GCTTGGGGAAGGAGTTCCCCTCCCAAGCCAGCCCGCAGAGGGTGAGGTCCCTGGAGGGGA
  TCCCACTGGCTCAGGTGGCTGCCGGAGGGGCTCACAGCTTTGCCCTGTCTCTCTGTGGGA
  CTTCGTTTGGCTGGGGAAGTAACAGTGCCGGGCAGCTGGCCCTCAGTGGGCGTAATGTCC
  CAGTGCAAAGCAACAAGCCTCTCTCAGTCGGTGCACTGAAGAATCTAGGTGTGGTTTATA
  TCAGCTGTGGTGATGCACACACTGCGGTGCTTACCCAGGACGGGAAAGTGTTCACATTTG
  GAGACAATCGCTCTGGACAGCTGGGATACAGCCCCACTCCTGAGAAGAGAGGTCCACAAC
  TTGTGGAAAGAATTGATGGCCTAGTTTCGCAGATAGATTGTGGAAGTTATCACACCCTGG
  CATATGTGCACACCACTGGTCAGGTGGTATCTTTTGGTCATGGACCAAGTGACACAAGCA
  AGCCAACTCATCCGGAGGCCCTGACAGAGAACTTTGACATTAGCTGCCTGATTTCTGCTG
  AAGACTTCGTGGATGTTCAAGTCAAACACATTTTTGCTGGAACATATGCCAACTTTGTGA
  CAACTCATCAGGATACTAGTTCCACACGTGCTCCCGGGAAAACCCTGCCAGAAATAAGCC
  GAATTAGCCAGTCCATGGCAGAAAAATGGATAGCAGTGAAAAGAAGAAGTACTGAACATG
  AAATGGCTAAAAGTGAAATTAGAATGATATTTTCATCTCCTGCTTGTCTGACTGCAAG:T
  TTTTTAAAGAAAAGAGGAACTGGAGAAACGACTTCCATTGATGTGGACTTAGAAATGGCA
  AGAGATACCTTCAAGAAGTTAACAAAAAAGGAATGGATTTCTTCCATGATAACTACGTGT
  CTCGAGGATGATCTGCTCAGAGCTCTTCCATGCCATTCTCCACACCAAGAAGCTTTATCA
  GTTTTCCTCCTGCTCCCAGAATGTCCTGTGATGCATGATTCTAAGAACTGGAAGAACCTG
  GTGGTTCCATTTGCAAAGGCTGTGTGTGAAATGAGTAAACAATCTTTGCAAGTCCTAAAG
  AAGTGTTGGGCATTTTTGCAAGAATCTTCTCTGAATCCGCTGATCCAGATGCTTAAAGCA
  GCCATCATCTCTCAGCTGCTTCATCAGACTAAAACCGAACAGGATCACTGTAATGTTAAA
  GCTCTTTTAGGAATGATGAAAGAACTGCATAAGGTAAACAAAGCTAACTGTCGACTACCA
  GAAAATACTTTCAACATAAATGAACTCTCCAACTTATTAAACTTTTATATAGATAGAGGA
  AGACAGCTCTTTCGGGATAACCACCTGATACCTGCAGAAACCCCCAGTCCTGTTATTTTC
  AGTGATTTTCCATTTATCTTTAATTCGCTATCCAAAATTAAATTATTGCAAGCTGATTCA
  CATATAAAGATGCAGATGTCAGAAAAGAAAGCATACATGCTTATGCATGAAACAATTCTG
  CAAAAAAAGGATGAATTTCCTCCATCACCCAGATTTATACTTAGAGTCAGACGAAGTCGC
  CTGGTTAAAGATGCTCTGCGTCAATTAAGTCAAGCTGAAGCTACTGACTTCTGCAAAGTA
  TTAGTGGTTGAATTTATTAATGAAATTTGTCCTGAGTCTGGAGGGGTTAGTTCAGAGTTC
  TTCCACTGTATGTTTGAAGAGATGACCAAGCCAGAATATGGAATGTTCATGTATCCTGAA
  ATGGGTTCCTGCATGTGGTTTCCTGCCAAGCCTAAACCTGAGAAGAAAAGATATTTCCTC
  TTTGGAATGCTGTGTGGACTCTCCTTATTCAATTTAAATGTTGCTAACCTTCCTTTCCCA
  CTGGCTCTGTATAAAAAACTTCTGGACCAAAAGCCATCATTGGAAGATTTAAAAGAACTC
  AGTCCTCGGTTGGGGAAGAGTTTGCAAGAAGTTCTAGATGATGCTGCTGATGACATTGGA
  GATGCGCTCTGCATACGCTTTTCTATACACTGGGACCAAAATGATGTTGACTTAATTCCA
  AATGGGATCTCCATACCTGTGGACCAAACCAACAAGAGAGACTATGTTTCTAAGTATATT
  GATTACATTTTCAACGTCTCTGTAAAAGCAGTTTATGAGGAATTTCAGAGAGGATTTTAT
  AGAGTCTGTGAGAAGGAGATACTTAGACATTTCTACCCTGAAGAACTAATGACAGCAATC
  ATTGGAAATACTGATTATGACTGGAAACAGTTTGAACAGAATTCAAAGTATGAGCAAGGA
  TACCAAAAATCACATCCTACTATACAGTTGTTTTGGAAGGCTTTCCACAAACTAACCTTG
  GATGAAAAGAAAAAATTCCTCTTTTTCCTTACAGGACGTGATAGGCTGCATGCAAGAGGC
  ATACAGAAAATGGAAATAGTATTTCGCTGTCCTGAAACTTTCAGTGAAAGAGATCACCCA
  ACATCAATAACTTGTCATAATATTCTCTCCCTCCCTAAGTATTCTACAATGGAAAGAATG
  GAGGAAGCACTTCAAGTAGCCATCAACAACAACAGAGGATTTGTCTCACCCATGCTCACA
  CAGTCATAATCACCTCTGAGAGACTCAGGGTGGGCTTTCTCACACTTGGATCCTTCTGTT
  CTTCCTTACACCTAAATAATACAAGAGATTAATGAATAGTGGTTAGAAGTAGTTGAGGGA
  GAGATTGGGGGAATGGGGAGATGATGATGATGGTCAAAGGGTGCAAAATCTCACACAAGA
  CTGAGGCAGGAGAATAGGGTACAGAGATAGGGATCTAAGGATGACTTGGACACACTCCCT
  GGCACTGAAGAGTCTGAACACTGGCCTGTGATTGGTCCATTCCAGGACCTTCATTTGCAT
  AAGGTATCAAACCACATCAGCCTCTGATTGGCCATGGGCCAGACCTGCACTCTGGCCAAT
  GATTGGTTCATTCCAGGACATTCATTTGCATAAGGAGTCAAACCACACCAGTCTTGGATT
  GGCTGTGAGCCAATTCACCTCAGTCTCTAATTGGCTGTGAGTCAGTCTTTCATTTACATA
  GGGTGTAACCATCAAGAAACCTCTACAGGGTACTTAAGCCCCAGAAGATTTTGCTACCAG
  GGCTCTTGAGCCACTTGCTCTAGCCCACTCCCACCCTGTGGAATGTACTTTCACTTTTGC
  TGCTTCACTGCCTTGTGCTCCAATAAATCCACTCCTTCACCACCCAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_017912
• Insert Size: 3900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017912.3, NP_060382.3
• RefSeq Size: 3903 bp
• RefSeq ORF: 3069 bp
• Locus ID: 55008
• UniProt ID: Q8IVU3
• Cytogenetics: 4q22.1
• Domains: HECT
• Protein Families: Druggable Genome
• Gene Summary: HERC6 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).