HERC6 (NM_017912) Human Tagged ORF Clone

CAT#: RG218907

HERC6 (tGFP-tagged) - Human hect domain and RLD 6 (HERC6), transcript variant 1

Product Images

Circular map for RG218907

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: HERC6
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG218907 representing NM_017912
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTACTTCTGTTGGGGCGCCGACTCCAGGGAGCTGCAGCGCCGGAGGACGGCGGGCAGCCCCGGGGCTG
  AGCTACTGCAGGCGGCCAGCGGGGAGCGCCACTCTCTGCTGCTGCTGACCAACCACAGGGTCCTCTCGTG
  CGGAGACAACAGCAGGGGTCAGCTGGGCCGCAGGGGCGCGCAGCGCGGGGAGCTGCCAGAACCAATTCAG
  GCATTGGAAACCCTAATTGTTGATCTCGTGAGCTGCGGGAAGGAGCACTCCCTGGCTGTGTGCCACAAAG
  GAAGGGTCTTCGCATGGGGAGCTGGTTCTGAAGGGCAGCTGGGGATTGGAGAATTCAAGGAAATAAGTTT
  CACACCTAAGAAAATAATGACTCTGAATGATATAAAAATAATACAAGTTTCCTGTGGACACTACCACTCC
  CTGGCATTATCAAAAGATAGCCAAGTGTTTTCGTGGGGAAAGAACAGCCATGGGCAGCTGGGCTTGGGGA
  AGGAGTTCCCCTCCCAAGCCAGCCCGCAGAGGGTGAGGTCCCTGGAGGGGATCCCACTGGCTCAGGTGGC
  TGCCGGAGGGGCTCACAGCTTTGCCCTGTCTCTCTGTGGGACTTCGTTTGGCTGGGGAAGTAACAGTGCC
  GGGCAGCTGGCCCTCAGTGGGCGTAATGTCCCAGTGCAAAGCAACAAGCCTCTCTCAGTCGGTGCACTGA
  AGAATCTAGGTGTGGTTTATATCAGCTGTGGTGATGCACACACTGCGGTGCTTACCCAGGACGGGAAAGT
  GTTCACATTTGGAGACAATCGCTCTGGACAGCTGGGATACAGCCCCACTCCTGAGAAGAGAGGTCCACAA
  CTTGTGGAAAGAATTGATGGCCTAGTTTCGCAGATAGATTGTGGAAGTTATCACACCCTGGCATATGTGC
  ACACCACTGGTCAGGTGGTATCTTTTGGTCATGGACCAAGTGACACAAGCAAGCCAACTCATCCGGAGGC
  CCTGACAGAGAACTTTGACATTAGCTGCCTGATTTCTGCTGAAGACTTCGTGGATGTTCAAGTCAAACAC
  ATTTTTGCTGGAACATATGCCAACTTTGTGACAACTCATCAGGATACTAGTTCCACACGTGCTCCCGGGA
  AAACCCTGCCAGAAATAAGCCGAATTAGCCAGTCCATGGCAGAAAAATGGATAGCAGTGAAAAGAAGAAG
  TACTGAACATGAAATGGCTAAAAGTGAAATTAGAATGATATTTTCATCTCCTGCTTGTCTGACTGCAAGT
  TTTTTAAAGAAAAGAGGAACTGGAGAAACGACTTCCATTGATGTGGACTTAGAAATGGCAAGAGATACCT
  TCAAGAAGTTAACAAAAAAGGAATGGATTTCTTCCATGATAACTACGTGTCTCGAGGATGATCTGCTCAG
  AGCTCTTCCATGCCATTCTCCACACCAAGAAGCTTTATCAGTTTTCCTCCTGCTCCCAGAATGTCCTGTG
  ATGCATGATTCTAAGAACTGGAAGAACCTGGTGGTTCCATTTGCAAAGGCTGTGTGTGAAATGAGTAAAC
  AATCTTTGCAAGTCCTAAAGAAGTGTTGGGCATTTTTGCAAGAATCTTCTCTGAATCCGCTGATCCAGAT
  GCTTAAAGCAGCCATCATCTCTCAGCTGCTTCATCAGACTAAAACCGAACAGGATCACTGTAATGTTAAA
  GCTCTTTTAGGAATGATGAAAGAACTGCATAAGGTAAACAAAGCTAACTGTCGACTACCAGAAAATACTT
  TCAACATAAATGAACTCTCCAACTTATTAAACTTTTATATAGATAGAGGAAGACAGCTCTTTCGGGATAA
  CCACCTGATACCTGCAGAAACCCCCAGTCCTGTTATTTTCAGTGATTTTCCATTTATCTTTAATTCGCTA
  TCCAAAATTAAATTATTGCAAGCTGATTCACATATAAAGATGCAGATGTCAGAAAAGAAAGCATACATGC
  TTATGCATGAAACAATTCTGCAAAAAAAGGATGAATTTCCTCCATCACCCAGATTTATACTTAGAGTCAG
  ACGAAGTCGCCTGGTTAAAGATGCTCTGCGTCAATTAAGTCAAGCTGAAGCTACTGACTTCTGCAAAGTA
  TTAGTGGTTGAATTTATTAATGAAATTTGTCCTGAGTCTGGAGGGGTTAGTTCAGAGTTCTTCCACTGTA
  TGTTTGAAGAGATGACCAAGCCAGAATATGGAATGTTCATGTATCCTGAAATGGGTTCCTGCATGTGGTT
  TCCTGCCAAGCCTAAACCTGAGAAGAAAAGATATTTCCTCTTTGGAATGCTGTGTGGACTCTCCTTATTC
  AATTTAAATGTTGCTAACCTTCCTTTCCCACTGGCTCTGTATAAAAAACTTCTGGACCAAAAGCCATCAT
  TGGAAGATTTAAAAGAACTCAGTCCTCGGTTGGGGAAGAGTTTGCAAGAAGTTCTAGATGATGCTGCTGA
  TGACATTGGAGATGCGCTCTGCATACGCTTTTCTATACACTGGGACCAAAATGATGTTGACTTAATTCCA
  AATGGGATCTCCATACCTGTGGACCAAACCAACAAGAGAGACTATGTTTCTAAGTATATTGATTACATTT
  TCAACGTCTCTGTAAAAGCAGTTTATGAGGAATTTCAGAGAGGATTTTATAGAGTCTGTGAGAAGGAGAT
  ACTTAGACATTTCTACCCTGAAGAACTAATGACAGCAATCATTGGAAATACTGATTATGACTGGAAACAG
  TTTGAACAGAATTCAAAGTATGAGCAAGGATACCAAAAATCACATCCTACTATACAGTTGTTTTGGAAGG
  CTTTCCACAAACTAACCTTGGATGAAAAGAAAAAATTCCTCTTTTTCCTTACAGGACGTGATAGGCTGCA
  TGCAAGAGGCATACAGAAAATGGAAATAGTATTTCGCTGTCCTGAAACTTTCAGTGAAAGAGATCACCCA
  ACATCAATAACTTGTCATAATATTCTCTCCCTCCCTAAGTATTCTACAATGGAAAGAATGGAGGAAGCAC
  TTCAAGTAGCCATCAACAACAACAGAGGATTTGTCTCACCCATGCTCACACAGTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG218907 representing NM_017912
  Red=Cloning site Green=Tags(s)
  
  MYFCWGADSRELQRRRTAGSPGAELLQAASGERHSLLLLTNHRVLSCGDNSRGQLGRRGAQRGELPEPIQ
  ALETLIVDLVSCGKEHSLAVCHKGRVFAWGAGSEGQLGIGEFKEISFTPKKIMTLNDIKIIQVSCGHYHS
  LALSKDSQVFSWGKNSHGQLGLGKEFPSQASPQRVRSLEGIPLAQVAAGGAHSFALSLCGTSFGWGSNSA
  GQLALSGRNVPVQSNKPLSVGALKNLGVVYISCGDAHTAVLTQDGKVFTFGDNRSGQLGYSPTPEKRGPQ
  LVERIDGLVSQIDCGSYHTLAYVHTTGQVVSFGHGPSDTSKPTHPEALTENFDISCLISAEDFVDVQVKH
  IFAGTYANFVTTHQDTSSTRAPGKTLPEISRISQSMAEKWIAVKRRSTEHEMAKSEIRMIFSSPACLTAS
  FLKKRGTGETTSIDVDLEMARDTFKKLTKKEWISSMITTCLEDDLLRALPCHSPHQEALSVFLLLPECPV
  MHDSKNWKNLVVPFAKAVCEMSKQSLQVLKKCWAFLQESSLNPLIQMLKAAIISQLLHQTKTEQDHCNVK
  ALLGMMKELHKVNKANCRLPENTFNINELSNLLNFYIDRGRQLFRDNHLIPAETPSPVIFSDFPFIFNSL
  SKIKLLQADSHIKMQMSEKKAYMLMHETILQKKDEFPPSPRFILRVRRSRLVKDALRQLSQAEATDFCKV
  LVVEFINEICPESGGVSSEFFHCMFEEMTKPEYGMFMYPEMGSCMWFPAKPKPEKKRYFLFGMLCGLSLF
  NLNVANLPFPLALYKKLLDQKPSLEDLKELSPRLGKSLQEVLDDAADDIGDALCIRFSIHWDQNDVDLIP
  NGISIPVDQTNKRDYVSKYIDYIFNVSVKAVYEEFQRGFYRVCEKEILRHFYPEELMTAIIGNTDYDWKQ
  FEQNSKYEQGYQKSHPTIQLFWKAFHKLTLDEKKKFLFFLTGRDRLHARGIQKMEIVFRCPETFSERDHP
  TSITCHNILSLPKYSTMERMEEALQVAINNNRGFVSPMLTQS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017912
• ORF Size: 3066 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017912.4
• RefSeq Size: 3903 bp
• RefSeq ORF: 3069 bp
• Locus ID: 55008
• UniProt ID: Q8IVU3
• Cytogenetics: 4q22.1
• Domains: HECT
• Protein Families: Druggable Genome
• Gene Summary: HERC6 belongs to the HERC family of ubiquitin ligases, all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate, and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al., 2005 [PubMed 15676274]).[supplied by OMIM, Mar 2008]