SAPS1 (PPP6R1) (NM_014931) Human Untagged Clone

CAT#: SC318355

PPP6R1 (untagged)-Human protein phosphatase 6, regulatory subunit 1 (PPP6R1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SAPS1
• Synonyms: KIAA1115; PP6R1; SAP190; SAPS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318355 representing NM_014931.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTTTTGGAAGTTTGACCTGCACACAAGCTCGCACCTGGACACGCTGCTGGAGCGGGAGGACCTGAGC
  CTGCCCGAGCTGCTGGACGAGGAAGACGTGCTGCAGGAGTGCAAGGTCGTCAACCGCAAGCTGCTGGAC
  TTCCTGCTGCAGCCACCCCACCTGCAAGCAATGGTGGCCTGGGTCACCCAGGAGCCGCCAGATAGCGGT
  GAGGAGCGGCTGCGCTACAAGTACCCCAGTGTGGCCTGCGAGATTCTGACCTCAGATGTGCCCCAGATC
  AATGATGCCCTGGGTGCTGATGAGTCCCTTCTGAACCGGCTCTACGGCTTCCTGCAGAGCACCGGCAGC
  CTCAACCCACTGCTGGCCAGCTTCTTCAGCAAGGTCATGGGCATCCTCATCAACCGCAAGACAGACCAG
  CTCGTGTCCTTTCTTCGGAAGAAGGATGACTTCGTGGACCTGCTGCTGCAGCACATTGGCACCTCGGCC
  ATCATGGACCTCCTGCTGCGCCTGCTCACCTGTGTGGAGCGGCCTCAGCTGAGGCAGGATGTTGTCAAT
  TGGCTCAACGAGGAGAAGATCGTCCAGCGGCTGATTGAGCAGATCCACCCGTCGAAGGATGAGAATCAA
  CATTCCAACGCATCCCAGTCCCTGTGTGACATCATCCGCCTGAGCCGGGAGCAGATGATCCAAGTCCAG
  GACAGCCCAGAGCCTGACCAACTGCTGGCCACCCTGGAGAAGCAGGAGACGATTGAGCAGCTCTTAAGC
  AACATGTTCGAGGGGGAGCAGAGCCAGTCTGTCATCGTCAGTGGGATCCAGGTGCTGCTGACCCTGCTG
  GAGCCCAGGAGGCCGAGGTCCGAGTCCGTGACCGTGAACAGCTTCTTCAGCAGTGTGGATGGGCAGCTG
  GAGCTCCTGGCCCAGGGGGCCCTGGAAAGCACTGTGTCCAGTGTGGGCGCCTTGCACGCCCTACGCCCG
  CGGCTCAGCTGCTTCCACCAGCTCCTGCTGGAGCCTCCCAAGCTGGAGCCGCTACAGATGACATGGGGC
  ATGCTGGCTCCGCCTCTGGGCAACACGCGGCTGCACGTGGTCAAGCTCCTGGCCAGTGCCCTGAGCGCC
  AATGATGCAGCCCTGACGCACGAGCTCCTGGCACTGGACGTGCCCAACACCATGCTGGACCTCTTCTTC
  CATTATGTCTTCAACAACTTCTTGCATGCCCAAGTAGAGGGATGCGTGAGCACCATGCTGAGCTTGGGG
  CCACCTCCTGACAGCAGCCCTGAGACGCCCATCCAAAACCCTGTTGTGAAACATCTGCTGCAGCAGTGC
  CGCCTGGTGGAGCGGATCCTGACGTCCTGGGAGGAGAACGACCGTGTACAGTGTGCGGGAGGCCCTCGG
  AAAGGCTACATGGGTCACCTGACAAGAGTGGCCGGTGCCCTGGTGCAGAACACGGAGAAGGGGCCCAAT
  GCAGAGCAGCTGCGGCAGCTGCTGAAGGAGCTGCCCAGCGAGCAGCAGGAGCAGTGGGAAGCCTTCGTA
  TCGGGGCCCCTGGCGGAGACCAACAAGAAGAACATGGTGGACCTGGTGAACACCCACCACCTACACTCC
  TCCAGTGACGATGAGGACGACCGGCTCAAGGAGTTCAACTTCCCTGAGGAGGCTGTGCTGCAGCAGGCC
  TTCATGGACTTCCAGATGCAGCGCATGACCTCTGCCTTCATTGACCACTTCGGCTTCAATGATGAGGAG
  TTTGGGGAGCAGGAGGAGAGTGTGAACGCACCTTTTGACAAGACAGCCAACATCACCTTCTCCCTCAAT
  GCTGACGATGAGAACCCCAACGCCAACCTACTTGAGATATGCTACAAGGACCGCATCCAGCAGTTTGAT
  GATGATGAGGAAGAGGAGGACGAGGAAGAGGCCCAGGGCTCAGGGGAGTCTGATGGAGAAGATGGCGCC
  TGGCAGGGCAGCCAGCTGGCCAGGGGGGCCCGTCTGGGCCAGCCACCTGGTGTCCGGAGTGGAGGCAGC
  ACAGACAGTGAGGACGAAGAAGAGGAGGACGAGGAGGAGGAGGAAGACGAGGAGGGCATTGGCTGTGCA
  GCCCGTGGAGGGGCCACCCCTCTGTCCTACCCCAGCCCTGGCCCTCAGCCTCCAGGCCCCAGCTGGACA
  GCCACCTTTGACCCAGTGCCTACAGATGCCCCGACCAGCCCCCGAGTCTCCGGGGAGGAAGAGCTGCAC
  ACTGGGCCTCCAGCCCCACAGGGGCCCCTCAGTGTGCCCCAGGGCCTCCCCACTCAGAGCCTGGCCAGC
  CCTCCTGCCCGTGACGCCCTGCAGCTCAGGTCTCAGGACCCCACACCCCCCTCAGCACCTCAGGAAGCC
  ACAGAAGGCAGCAAAGTCACGGAGCCCTCAGCCCCTTGCCAGGCCTTGGTTAGCATCGGGGACCTTCAG
  GCCACCTTCCACGGGATCCGTTCTGCCCCCAGCTCCTCGGACAGTGCAACCAGAGACCCCTCTACCTCT
  GTCCCAGCCTCCGGGGCCCACCAGCCCCCCCAGACCACAGAAGGGGAGAAGAGCCCAGAGCCCTTGGGG
  CTTCCCCAAAGCCAGAGTGCCCAGGCCCTCACGCCTCCTCCGATACCCAATGGCTCTGCCCCGGAAGGG
  CCTGCATCCCCAGGCTCCCAATAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014931
• Insert Size: 2646 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014931.3
• RefSeq Size: 3654 bp
• RefSeq ORF: 2646 bp
• Locus ID: 22870
• UniProt ID: Q9UPN7
• Cytogenetics: 19q13.42
• Domains: SAPS
• MW: 96.7 kDa
• Gene Summary: Protein phosphatase regulatory subunits, such as SAPS1, modulate the activity of protein phosphatase catalytic subunits by restricting substrate specificity, recruiting substrates, and determining the intracellular localization of the holoenzyme. SAPS1 is a regulatory subunit for the protein phosphatase-6 catalytic subunit (PPP6C; MIM 612725) (Stefansson and Brautigan, 2006 [PubMed 16769727]).[supplied by OMIM, Nov 2010]