THSD7A (NM_015204) Human Untagged Clone

CAT#: SC316606

THSD7A (untagged)-Human thrombospondin, type I, domain containing 7A (THSD7A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: THSD7A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC316606 representing NM_015204.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGCTGCAAGCCAGGCGCTGGGCGTCCGGGAGCCGGGGCGCTGCGGGGCCGCGCCGGGGCGTCCTG
  CAGCTGCTGCCGCTGCCGCTGCCGCTGCCGCTGCTCCTGCTGCTGCTGCTACGCCCGGGCGCCGGCAGG
  GCTGCGGCGCAGGGCGAGGCGGAGGCGCCCACCCTCTATCTGTGGAAGACTGGTCCATGGGGCCGATGT
  ATGGGAGATGAATGTGGTCCCGGAGGCATCCAAACGAGGGCTGTGTGGTGTGCTCATGTGGAGGGATGG
  ACTACACTGCATACTAACTGTAAGCAGGCCGAGAGACCCAATAACCAGCAGAATTGTTTCAAAGTTTGC
  GATTGGCACAAAGAGTTGTACGACTGGAGACTGGGACCTTGGAATCAGTGTCAGCCCGTGATTTCAAAA
  AGCCTAGAGAAACCTCTTGAGTGCATTAAGGGGGAAGAAGGTATTCAGGTGAGGGAGATAGCGTGCATC
  CAGAAAGACAAAGACATTCCTGCGGAGGATATCATCTGTGAGTACTTTGAGCCCAAGCCTCTCCTGGAG
  CAGGCTTGCCTCATTCCTTGCCAGCAAGATTGCATCGTGTCTGAATTTTCTGCCTGGTCCGAATGCTCC
  AAGACCTGCGGCAGCGGGCTCCAGCACCGGACGCGTCATGTGGTGGCGCCCCCGCAGTTCGGAGGCTCT
  GGCTGTCCAAACCTGACGGAGTTCCAGGTGTGCCAATCCAGTCCATGCGAGGCCGAGGAGCTCAGGTAC
  AGCCTGCATGTGGGGCCCTGGAGCACCTGCTCAATGCCCCACTCCCGACAAGTAAGACAAGCAAGGAGA
  CGCGGGAAGAATAAAGAACGGGAAAAGGACCGCAGCAAAGGAGTAAAGGATCCAGAAGCCCGCGAGCTT
  ATTAAGAAAAAGAGAAACAGAAACAGGCAGAACAGACAAGAGAACAAATATTGGGACATCCAGATTGGA
  TATCAGACCAGAGAGGTTATGTGCATTAACAAGACGGGGAAAGCTGCTGATTTAAGCTTTTGCCAGCAA
  GAGAAGCTTCCAATGACCTTCCAGTCCTGTGTGATCACCAAAGAGTGCCAGGTTTCCGAGTGGTCAGAG
  TGGAGCCCCTGCTCAAAAACATGCCATGACATGGTGTCCCCTGCAGGCACTCGTGTAAGGACACGAACC
  ATCAGGCAGTTTCCCATTGGCAGTGAAAAGGAGTGTCCAGAATTTGAAGAAAAAGAACCCTGTTTGTCT
  CAAGGAGATGGAGTTGTCCCCTGTGCCACGTATGGCTGGAGAACTACAGAGTGGACTGAGTGCCGTGTG
  GACCCTTTGCTCAGTCAGCAGGACAAGAGGCGCGGCAACCAGACGGCCCTCTGTGGAGGGGGCATCCAG
  ACCCGAGAGGTGTACTGCGTGCAGGCCAACGAAAACCTCCTCTCACAATTAAGTACCCACAAGAACAAA
  GAAGCCTCAAAGCCAATGGACTTAAAATTATGCACTGGACCTATCCCTAATACTACACAGCTGTGCCAC
  ATTCCTTGTCCAACTGAATGTGAAGTTTCACCTTGGTCAGCTTGGGGACCTTGTACTTATGAAAACTGT
  AATGATCAGCAAGGGAAAAAAGGCTTCAAACTGAGGAAGCGGCGCATTACCAATGAGCCCACTGGAGGC
  TCTGGGGTAACCGGAAACTGCCCTCACTTACTGGAAGCCATTCCCTGTGAAGAGCCTGCCTGTTATGAC
  TGGAAAGCAGTGAGACTGGGAAACTGCGAGCCAGATAACGGAAAGGAGTGTGGTCCAGGCACGCAAGTT
  CAAGAGGTTGTGTGCATCAACAGTGATGGAGAAGAAGTTGACAGACAGCTGTGCAGAGATGCCATCTTC
  CCCATCCCTGTGGCCTGTGATGCCCCATGCCCGAAAGACTGTGTGCTCAGCACATGGTCTACGTGGTCC
  TCCTGCTCACACACCTGCTCAGGGAAAACGACAGAAGGGAAACAGATACGAGCACGATCCATTCTGGCC
  TATGCGGGTGAAGAAGGTGGAATTCGCTGTCCAAATAGCAGTGCTTTGCAAGAAGTACGAAGCTGTAAT
  GAGCATCCTTGCACAGTGTACCACTGGCAAACTGGTCCCTGGGGCCAGTGCATTGAGGACACCTCAGTA
  TCGTCCTTCAACACAACTACGACTTGGAATGGGGAGGCCTCCTGCTCTGTCGGCATGCAGACAAGAAAA
  GTCATCTGTGTGCGAGTCAATGTGGGCCAAGTGGGACCCAAAAAATGTCCTGAAAGCCTTCGACCTGAA
  ACTGTAAGGCCTTGTCTGCTTCCTTGTAAGAAGGACTGTATTGTGACCCCATATAGTGACTGGACATCA
  TGCCCCTCTTCGTGTAAAGAAGGGGACTCCAGTATCAGGAAGCAGTCTAGGCATCGGGTCATCATTCAG
  CTGCCAGCCAACGGGGGCCGAGACTGCACAGATCCCCTCTATGAAGAGAAGGCCTGTGAGGCACCTCAA
  GCGTGCCAAAGCTACAGGTGGAAGACTCACAAATGGCGCAGATGCCAATTAGTCCCTTGGAGCGTGCAA
  CAAGACAGCCCTGGAGCACAGGAAGGCTGTGGGCCTGGGCGACAGGCAAGAGCCATTACTTGTCGCAAG
  CAAGATGGAGGACAGGCTGGAATCCATGAGTGCCTACAGTATGCAGGCCCTGTGCCAGCCCTTACCCAG
  GCCTGCCAGATCCCCTGCCAGGATGACTGTCAATTGACCAGCTGGTCCAAGTTTTCTTCATGCAATGGA
  GACTGTGGTGCAGTTAGGACCAGAAAGCGCACTCTTGTTGGAAAAAGTAAAAAGAAGGAAAAATGTAAA
  AATTCCCATTTGTATCCCCTGATTGAGACTCAGTATTGTCCTTGTGACAAATATAATGCACAACCTGTG
  GGGAACTGGTCAGACTGTATTTTACCAGAGGGAAAAGTGGAAGTGTTGCTGGGAATGAAAGTACAAGGA
  GACATCAAGGAATGCGGACAAGGATATCGTTACCAAGCAATGGCATGCTACGATCAAAATGGCAGGCTT
  GTGGAAACATCTAGATGTAACAGCCATGGTTACATTGAGGAGGCCTGCATCATCCCCTGCCCCTCAGAC
  TGCAAGCTCAGTGAGTGGTCCAACTGGTCGCGCTGCAGCAAGTCCTGTGGGAGTGGTGTGAAGGTTCGT
  TCTAAATGGCTGCGTGAAAAACCATATAATGGAGGAAGGCCTTGCCCCAAACTGGACCATGTCAACCAG
  GCACAGGTGTATGAGGTTGTCCCATGCCACAGTGACTGCAACCAGTACCTATGGGTCACAGAGCCCTGG
  AGCATCTGCAAGGTGACCTTTGTGAATATGCGGGAGAACTGTGGAGAGGGCGTGCAAACCCGAAAAGTG
  AGATGCATGCAGAATACAGCAGATGGCCCTTCTGAACATGTAGAGGATTACCTCTGTGACCCAGAAGAG
  ATGCCCCTGGGCTCTAGAGTGTGCAAATTACCATGCCCTGAGGACTGTGTGATATCTGAATGGGGTCCA
  TGGACCCAATGTGTTTTGCCTTGCAATCAAAGCAGTTTCCGGCAAAGGTCAGCTGATCCCATCAGACAA
  CCAGCTGATGAAGGAAGATCTTGCCCTAATGCTGTTGAGAAAGAACCCTGTAACCTGAACAAAAACTGC
  TACCACTATGATTATAATGTAACAGACTGGAGTACATGTCAGCTGAGTGAGAAGGCAGTTTGTGGAAAT
  GGAATAAAAACAAGGATGTTGGATTGTGTTCGAAGTGATGGCAAGTCAGTTGACCTGAAATATTGTGAA
  GCGCTTGGCTTGGAGAAGAACTGGCAGATGAACACGTCCTGCATGGTGGAATGCCCTGTGAACTGTCAG
  CTTTCTGATTGGTCTCCTTGGTCAGAATGTTCTCAAACATGTGGCCTCACAGGAAAAATGATCCGAAGA
  CGAACAGTGACCCAGCCCTTTCAAGGTGATGGAAGACCATGCCCTTCCCTGATGGACCAGTCCAAACCC
  TGCCCAGTGAAGCCTTGTTATCGGTGGCAATATGGCCAGTGGTCTCCATGCCAAGTGCAGGAGGCCCAG
  TGTGGAGAAGGGACCAGAACAAGGAACATTTCTTGTGTAGTAAGTGATGGGTCAGCTGATGATTTCAGC
  AAAGTGGTGGATGAGGAATTCTGTGCTGACATTGAACTCATTATAGATGGTAATAAAAATATGGTTCTG
  GAGGAATCCTGCAGCCAGCCTTGCCCAGGTGACTGTTATTTGAAGGACTGGTCTTCCTGGAGCCTGTGT
  CAGCTGACCTGTGTGAATGGTGAGGATCTAGGCTTTGGTGGAATACAGGTCAGATCCAGACCGGTGATT
  ATACAAGAACTAGAGAATCAGCATCTGTGCCCAGAGCAGATGTTAGAAACAAAATCATGTTATGATGGA
  CAGTGCTATGAATATAAATGGATGGCCAGTGCTTGGAAGGGCTCTTCCCGAACAGTGTGGTGTCAAAGG
  TCAGATGGTATAAATGTAACAGGGGGCTGCTTGGTGATGAGCCAGCCTGATGCCGACAGGTCTTGTAAC
  CCACCGTGTAGTCAACCCCACTCGTACTGTAGCGAGACAAAAACATGCCATTGTGAAGAAGGGTACACT
  GAAGTCATGTCTTCTAACAGCACCCTTGAGCAATGCACACTTATCCCCGTGGTGGTATTACCCACCATG
  GAGGACAAAAGAGGAGATGTGAAAACCAGTCGGGCTGTACATCCAACCCAACCCTCCAGTAACCCAGCA
  GGACGGGGAAGGACCTGGTTTCTACAGCCATTTGGGCCAGATGGGAGACTAAAGACCTGGGTTTACGGT
  GTAGCAGCTGGGGCATTTGTGTTACTCATCTTTATTGTCTCCATGATTTATCTAGCTTGCAAAAAGCCA
  AAGAAACCCCAAAGAAGGCAAAACAACCGACTGAAACCTTTAACCTTAGCCTATGATGGAGATGCCGAC
  ATGTAA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_015204
• Insert Size: 4974 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015204.2
• RefSeq Size: 10607 bp
• RefSeq ORF: 4974 bp
• Locus ID: 221981
• UniProt ID: Q9UPZ6
• Cytogenetics: 7p21.3
• Protein Families: Transmembrane
• MW: 185.4 kDa
• Gene Summary: The protein encoded by this gene is found almost exclusively in endothelial cells from placenta and umbilical cord. The encoded protein appears to interact with alpha(V)beta(3) integrin and paxillin to inhibit endothelial cell migration and tube formation. This protein may be involved in cytoskeletal organization. Variations in this gene may be associated with low bone mineral density in osteoporosis. [provided by RefSeq, Aug 2010]