THSD7A (NM_015204) Human Tagged ORF Clone

CAT#: RC213616

THSD7A (Myc-DDK-tagged)-Human thrombospondin, type I, domain containing 7A (THSD7A)

Product Images

Circular map for RC213616

• 

  Western blot validation of overexpression lysate (Cat# LY414726) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC213616 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: THSD7A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213616 representing NM_015204
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCTGCAAGCCAGGCGCTGGGCGTCCGGGAGCCGGGGCGCTGCGGGGCCGCGCCGGGGCGTCCTGC
  AGCTGCTGCCGCTGCCGCTGCCGCTGCCGCTGCTCCTGCTGCTGCTGCTACGCCCGGGCGCCGGCAGGGC
  TGCGGCGCAGGGCGAGGCGGAGGCGCCCACCCTCTATCTGTGGAAGACTGGTCCATGGGGCCGATGTATG
  GGAGATGAATGTGGTCCCGGAGGCATCCAAACGAGGGCTGTGTGGTGTGCTCATGTGGAGGGATGGACTA
  CACTGCATACTAACTGTAAGCAGGCCGAGAGACCCAATAACCAGCAGAATTGTTTCAAAGTTTGCGATTG
  GCACAAAGAGTTGTACGACTGGAGACTGGGACCTTGGAATCAGTGTCAGCCCGTGATTTCAAAAAGCCTA
  GAGAAACCTCTTGAGTGCATTAAGGGGGAAGAAGGTATTCAGGTGAGGGAGATAGCGTGCATCCAGAAAG
  ACAAAGACATTCCTGCGGAGGATATCATCTGTGAGTACTTTGAGCCCAAGCCTCTCCTGGAGCAGGCTTG
  CCTCATTCCTTGCCAGCAAGATTGCATCGTGTCTGAATTTTCTGCCTGGTCCGAATGCTCCAAGACCTGC
  GGCAGCGGGCTCCAGCACCGGACGCGTCATGTGGTGGCGCCCCCGCAGTTCGGAGGCTCTGGCTGTCCAA
  ACCTGACGGAGTTCCAGGTGTGCCAATCCAGTCCATGCGAGGCCGAGGAGCTCAGGTACAGCCTGCATGT
  GGGGCCCTGGAGCACCTGCTCAATGCCCCACTCCCGACAAGTAAGACAAGCAAGGAGACGCGGGAAGAAT
  AAAGAACGGGAAAAGGACCGCAGCAAAGGAGTAAAGGATCCAGAAGCCCGCGAGCTTATTAAGAAAAAGA
  GAAACAGAAACAGGCAGAACAGACAAGAGAACAAATATTGGGACATCCAGATTGGATATCAGACCAGAGA
  GGTTATGTGCATTAACAAGACGGGGAAAGCTGCTGATTTAAGCTTTTGCCAGCAAGAGAAGCTTCCAATG
  ACCTTCCAGTCCTGTGTGATCACCAAAGAGTGCCAGGTTTCCGAGTGGTCAGAGTGGAGCCCCTGCTCAA
  AAACATGCCATGACATGGTGTCCCCTGCAGGCACTCGTGTAAGGACACGAACCATCAGGCAGTTTCCCAT
  TGGCAGTGAAAAGGAGTGTCCAGAATTTGAAGAAAAAGAACCCTGTTTGTCTCAAGGAGATGGAGTTGTC
  CCCTGTGCCACGTATGGCTGGAGAACTACAGAGTGGACTGAGTGCCGTGTGGACCCTTTGCTCAGTCAGC
  AGGACAAGAGGCGCGGCAACCAGACGGCCCTCTGTGGAGGGGGCATCCAGACCCGAGAGGTGTACTGCGT
  GCAGGCCAACGAAAACCTCCTCTCACAATTAAGTACCCACAAGAACAAAGAAGCCTCAAAGCCAATGGAC
  TTAAAATTATGCACTGGACCTATCCCTAATACTACACAGCTGTGCCACATTCCTTGTCCAACTGAATGTG
  AAGTTTCACCTTGGTCAGCTTGGGGACCTTGTACTTATGAAAACTGTAATGATCAGCAAGGGAAAAAAGG
  CTTCAAACTGAGGAAGCGGCGCATTACCAATGAGCCCACTGGAGGCTCTGGGGTAACCGGAAACTGCCCT
  CACTTACTGGAAGCCATTCCCTGTGAAGAGCCTGCCTGTTATGACTGGAAAGCAGTGAGACTGGGAAACT
  GCGAGCCAGATAACGGAAAGGAGTGTGGTCCAGGCACGCAAGTTCAAGAGGTTGTGTGCATCAACAGTGA
  TGGAGAAGAAGTTGACAGACAGCTGTGCAGAGATGCCATCTTCCCCATCCCTGTGGCCTGTGATGCCCCA
  TGCCCGAAAGACTGTGTGCTCAGCACATGGTCTACGTGGTCCTCCTGCTCACACACCTGCTCAGGGAAAA
  CGACAGAAGGGAAACAGATACGAGCACGATCCATTCTGGCCTATGCGGGTGAAGAAGGTGGAATTCGCTG
  TCCAAATAGCAGTGCTTTGCAAGAAGTACGAAGCTGTAATGAGCATCCTTGCACAGTGTACCACTGGCAA
  ACTGGTCCCTGGGGCCAGTGCATTGAGGACACCTCAGTATCGTCCTTCAACACAACTACGACTTGGAATG
  GGGAGGCCTCCTGCTCTGTCGGCATGCAGACAAGAAAAGTCATCTGTGTGCGAGTCAATGTGGGCCAAGT
  GGGACCCAAAAAATGTCCTGAAAGCCTTCGACCTGAAACTGTAAGGCCTTGTCTGCTTCCTTGTAAGAAG
  GACTGTATTGTGACCCCATATAGTGACTGGACATCATGCCCCTCTTCGTGTAAAGAAGGGGACTCCAGTA
  TCAGGAAGCAGTCTAGGCATCGGGTCATCATTCAGCTGCCAGCCAACGGGGGCCGAGACTGCACAGATCC
  CCTCTATGAAGAGAAGGCCTGTGAGGCACCTCAAGCGTGCCAAAGCTACAGGTGGAAGACTCACAAATGG
  CGCAGATGCCAATTAGTCCCTTGGAGCGTGCAACAAGACAGCCCTGGAGCACAGGAAGGCTGTGGGCCTG
  GGCGACAGGCAAGAGCCATTACTTGTCGCAAGCAAGATGGAGGACAGGCTGGAATCCATGAGTGCCTACA
  GTATGCAGGCCCTGTGCCAGCCCTTACCCAGGCCTGCCAGATCCCCTGCCAGGATGACTGTCAATTGACC
  AGCTGGTCCAAGTTTTCTTCATGCAATGGAGACTGTGGTGCAGTTAGGACCAGAAAGCGCACTCTTGTTG
  GAAAAAGTAAAAAGAAGGAAAAATGTAAAAATTCCCATTTGTATCCCCTGATTGAGACTCAGTATTGTCC
  TTGTGACAAATATAATGCACAACCTGTGGGGAACTGGTCAGACTGTATTTTACCAGAGGGAAAAGTGGAA
  GTGTTGCTGGGAATGAAAGTACAAGGAGACATCAAGGAATGCGGACAAGGATATCGTTACCAAGCAATGG
  CATGCTACGATCAAAATGGCAGGCTTGTGGAAACATCTAGATGTAACAGCCATGGTTACATTGAGGAGGC
  CTGCATCATCCCCTGCCCCTCAGACTGCAAGCTCAGTGAGTGGTCCAACTGGTCGCGCTGCAGCAAGTCC
  TGTGGGAGTGGTGTGAAGGTTCGTTCTAAATGGCTGCGTGAAAAACCATATAATGGAGGAAGGCCTTGCC
  CCAAACTGGACCATGTCAACCAGGCACAGGTGTATGAGGTTGTCCCATGCCACAGTGACTGCAACCAGTA
  CCTATGGGTCACAGAGCCCTGGAGCATCTGCAAGGTGACCTTTGTGAATATGCGGGAGAACTGTGGAGAG
  GGCGTGCAAACCCGAAAAGTGAGATGCATGCAGAATACAGCAGATGGCCCTTCTGAACATGTAGAGGATT
  ACCTCTGTGACCCAGAAGAGATGCCCCTGGGCTCTAGAGTGTGCAAATTACCATGCCCTGAGGACTGTGT
  GATATCTGAATGGGGTCCATGGACCCAATGTGTTTTGCCTTGCAATCAAAGCAGTTTCCGGCAAAGGTCA
  GCTGATCCCATCAGACAACCAGCTGATGAAGGAAGATCTTGCCCTAATGCTGTTGAGAAAGAACCCTGTA
  ACCTGAACAAAAACTGCTACCACTATGATTATAATGTAACAGACTGGAGTACATGTCAGCTGAGTGAGAA
  GGCAGTTTGTGGAAATGGAATAAAAACAAGGATGTTGGATTGTGTTCGAAGTGATGGCAAGTCAGTTGAC
  CTGAAATATTGTGAAGCGCTTGGCTTGGAGAAGAACTGGCAGATGAACACGTCCTGCATGGTGGAATGCC
  CTGTGAACTGTCAGCTTTCTGATTGGTCTCCTTGGTCAGAATGTTCTCAAACATGTGGCCTCACAGGAAA
  AATGATCCGAAGACGAACAGTGACCCAGCCCTTTCAAGGTGATGGAAGACCATGCCCTTCCCTGATGGAC
  CAGTCCAAACCCTGCCCAGTGAAGCCTTGTTATCGGTGGCAATATGGCCAGTGGTCTCCATGCCAAGTGC
  AGGAGGCCCAGTGTGGAGAAGGGACCAGAACAAGGAACATTTCTTGTGTAGTAAGTGATGGGTCAGCTGA
  TGATTTCAGCAAAGTGGTGGATGAGGAATTCTGTGCTGACATTGAACTCATTATAGATGGTAATAAAAAT
  ATGGTTCTGGAGGAATCCTGCAGCCAGCCTTGCCCAGGTGACTGTTATTTGAAGGACTGGTCTTCCTGGA
  GCCTGTGTCAGCTGACCTGTGTGAATGGTGAGGATCTAGGCTTTGGTGGAATACAGGTCAGATCCAGACC
  GGTGATTATACAAGAACTAGAGAATCAGCATCTGTGCCCAGAGCAGATGTTAGAAACAAAATCATGTTAT
  GATGGACAGTGCTATGAATATAAATGGATGGCCAGTGCTTGGAAGGGCTCTTCCCGAACAGTGTGGTGTC
  AAAGGTCAGATGGTATAAATGTAACAGGGGGCTGCTTGGTGATGAGCCAGCCTGATGCCGACAGGTCTTG
  TAACCCACCGTGTAGTCAACCCCACTCGTACTGTAGCGAGACAAAAACATGCCATTGTGAAGAAGGGTAC
  ACTGAAGTCATGTCTTCTAACAGCACCCTTGAGCAATGCACACTTATCCCCGTGGTGGTATTACCCACCA
  TGGAGGACAAAAGAGGAGATGTGAAAACCAGTCGGGCTGTACATCCAACCCAACCCTCCAGTAACCCAGC
  AGGACGGGGAAGGACCTGGTTTCTACAGCCATTTGGGCCAGATGGGAGACTAAAGACCTGGGTTTACGGT
  GTAGCAGCTGGGGCATTTGTGTTACTCATCTTTATTGTCTCCATGATTTATCTAGCTTGCAAAAAGCCAA
  AGAAACCCCAAAGAAGGCAAAACAACCGACTGAAACCTTTAACCTTAGCCTATGATGGAGATGCCGACAT
  G
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213616 representing NM_015204
  Red=Cloning site Green=Tags(s)
  
  MGLQARRWASGSRGAAGPRRGVLQLLPLPLPLPLLLLLLLRPGAGRAAAQGEAEAPTLYLWKTGPWGRCM
  GDECGPGGIQTRAVWCAHVEGWTTLHTNCKQAERPNNQQNCFKVCDWHKELYDWRLGPWNQCQPVISKSL
  EKPLECIKGEEGIQVREIACIQKDKDIPAEDIICEYFEPKPLLEQACLIPCQQDCIVSEFSAWSECSKTC
  GSGLQHRTRHVVAPPQFGGSGCPNLTEFQVCQSSPCEAEELRYSLHVGPWSTCSMPHSRQVRQARRRGKN
  KEREKDRSKGVKDPEARELIKKKRNRNRQNRQENKYWDIQIGYQTREVMCINKTGKAADLSFCQQEKLPM
  TFQSCVITKECQVSEWSEWSPCSKTCHDMVSPAGTRVRTRTIRQFPIGSEKECPEFEEKEPCLSQGDGVV
  PCATYGWRTTEWTECRVDPLLSQQDKRRGNQTALCGGGIQTREVYCVQANENLLSQLSTHKNKEASKPMD
  LKLCTGPIPNTTQLCHIPCPTECEVSPWSAWGPCTYENCNDQQGKKGFKLRKRRITNEPTGGSGVTGNCP
  HLLEAIPCEEPACYDWKAVRLGNCEPDNGKECGPGTQVQEVVCINSDGEEVDRQLCRDAIFPIPVACDAP
  CPKDCVLSTWSTWSSCSHTCSGKTTEGKQIRARSILAYAGEEGGIRCPNSSALQEVRSCNEHPCTVYHWQ
  TGPWGQCIEDTSVSSFNTTTTWNGEASCSVGMQTRKVICVRVNVGQVGPKKCPESLRPETVRPCLLPCKK
  DCIVTPYSDWTSCPSSCKEGDSSIRKQSRHRVIIQLPANGGRDCTDPLYEEKACEAPQACQSYRWKTHKW
  RRCQLVPWSVQQDSPGAQEGCGPGRQARAITCRKQDGGQAGIHECLQYAGPVPALTQACQIPCQDDCQLT
  SWSKFSSCNGDCGAVRTRKRTLVGKSKKKEKCKNSHLYPLIETQYCPCDKYNAQPVGNWSDCILPEGKVE
  VLLGMKVQGDIKECGQGYRYQAMACYDQNGRLVETSRCNSHGYIEEACIIPCPSDCKLSEWSNWSRCSKS
  CGSGVKVRSKWLREKPYNGGRPCPKLDHVNQAQVYEVVPCHSDCNQYLWVTEPWSICKVTFVNMRENCGE
  GVQTRKVRCMQNTADGPSEHVEDYLCDPEEMPLGSRVCKLPCPEDCVISEWGPWTQCVLPCNQSSFRQRS
  ADPIRQPADEGRSCPNAVEKEPCNLNKNCYHYDYNVTDWSTCQLSEKAVCGNGIKTRMLDCVRSDGKSVD
  LKYCEALGLEKNWQMNTSCMVECPVNCQLSDWSPWSECSQTCGLTGKMIRRRTVTQPFQGDGRPCPSLMD
  QSKPCPVKPCYRWQYGQWSPCQVQEAQCGEGTRTRNISCVVSDGSADDFSKVVDEEFCADIELIIDGNKN
  MVLEESCSQPCPGDCYLKDWSSWSLCQLTCVNGEDLGFGGIQVRSRPVIIQELENQHLCPEQMLETKSCY
  DGQCYEYKWMASAWKGSSRTVWCQRSDGINVTGGCLVMSQPDADRSCNPPCSQPHSYCSETKTCHCEEGY
  TEVMSSNSTLEQCTLIPVVVLPTMEDKRGDVKTSRAVHPTQPSSNPAGRGRTWFLQPFGPDGRLKTWVYG
  VAAGAFVLLIFIVSMIYLACKKPKKPQRRQNNRLKPLTLAYDGDADM
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015204
• ORF Size: 4971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015204.3
• RefSeq Size: 6487 bp
• RefSeq ORF: 4974 bp
• Locus ID: 221981
• UniProt ID: Q9UPZ6
• Cytogenetics: 7p21.3
• Protein Families: Transmembrane
• MW: 185.2 kDa
• Gene Summary: The protein encoded by this gene is found almost exclusively in endothelial cells from placenta and umbilical cord. The encoded protein appears to interact with alpha(V)beta(3) integrin and paxillin to inhibit endothelial cell migration and tube formation. This protein may be involved in cytoskeletal organization. Variations in this gene may be associated with low bone mineral density in osteoporosis. [provided by RefSeq, Aug 2010]